The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100261&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/130035 |
Resumo: | The aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21,45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers. |
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The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiencyOsteoporosisBone RegenerationBone ResorptionThe aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21,45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers.Univ Estadual Paulista UNESP, ICT, Dept Biosci &Oral Diag, Sao Jose Dos Campos, SP, BrazilUniv Estadual Paulista UNESP, ICT, Dept Diag &Surg, Sao Jose Dos Campos, SP, BrazilUniv Estadual Paulista UNESP, ICT, Dept Biosci &Oral Diag, Sao Jose Dos Campos, SP, BrazilUniv Estadual Paulista UNESP, ICT, Dept Diag &Surg, Sao Jose Dos Campos, SP, BrazilSociedade Brasileira De Pesquisa OdontologicaUniversidade Estadual Paulista (Unesp)Tera, Tabata de Mello [UNESP]Prado, Renata Falchete do [UNESP]Marco, Andrea Carvalho de [UNESP]Santamaria, Mauro Pedrine [UNESP]Neves Jardini, Maria Aparecida [UNESP]2015-11-03T15:28:45Z2015-11-03T15:28:45Z2014-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article469-477application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100261&lng=en&nrm=iso&tlng=enBrazilian Oral Research. Sao Paulo: Sociedade Brasileira De Pesquisa Odontologica, v. 28, n. 6, p. 469-477, 2014.1806-8324http://hdl.handle.net/11449/13003510.1590/1807-3107BOR-2014.vol28.0054S1806-83242014000100261WOS:000352327500009S1806-83242014000100261.pdf806991232173225870081149233979475733639342016958Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2024-01-06T06:31:13Zoai:repositorio.unesp.br:11449/130035Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-06T06:31:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
title |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
spellingShingle |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency Tera, Tabata de Mello [UNESP] Osteoporosis Bone Regeneration Bone Resorption |
title_short |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
title_full |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
title_fullStr |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
title_full_unstemmed |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
title_sort |
The RANK/RANKL/OPG interaction in the repair of autogenous bone grafts in female rats with estrogen deficiency |
author |
Tera, Tabata de Mello [UNESP] |
author_facet |
Tera, Tabata de Mello [UNESP] Prado, Renata Falchete do [UNESP] Marco, Andrea Carvalho de [UNESP] Santamaria, Mauro Pedrine [UNESP] Neves Jardini, Maria Aparecida [UNESP] |
author_role |
author |
author2 |
Prado, Renata Falchete do [UNESP] Marco, Andrea Carvalho de [UNESP] Santamaria, Mauro Pedrine [UNESP] Neves Jardini, Maria Aparecida [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Tera, Tabata de Mello [UNESP] Prado, Renata Falchete do [UNESP] Marco, Andrea Carvalho de [UNESP] Santamaria, Mauro Pedrine [UNESP] Neves Jardini, Maria Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Osteoporosis Bone Regeneration Bone Resorption |
topic |
Osteoporosis Bone Regeneration Bone Resorption |
description |
The aim of this study was to evaluate the resorption process during the repair of autogenous bone grafts with or without coverage by an expanded polytetrafluoroethylene (e-PTFE) membrane in female rats with estrogen deficiency using the immunohistochemical technique. Eighty female rats were randomly divided into two groups (OVX and SHAM). The 40 female rats in the OVX group were subjected to ovariectomy, and the 40 female rats in the SHAM group were subjected to simulated ovariectomy. The two groups were further divided in subgroup E, which was subjected to surgery for placement of autogenous bone graft (ABG), and subgroup ME, in which the ABG was covered with an e-PTFE membrane. The animals were killed at 0, 7, 21,45 and 60 days. The specimens were analyzed using immunohistochemistry for the bone resorption markers RANK, RANK-L and Osteoprotegerin (OPG). A higher remodeling rate was observed at 7 and 21 days after the autogenous bone grafts, when the markers were more intensely expressed. At the final time point, the specimens presented similar characteristics to those observed at the initial time point. The expression of immunohistochemical markers was not altered by the estrogen deficiency. The presence of the e-PTFE membrane delayed the bone resorption process, influencing the immunohistochemical expression of markers. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11-01 2015-11-03T15:28:45Z 2015-11-03T15:28:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100261&lng=en&nrm=iso&tlng=en Brazilian Oral Research. Sao Paulo: Sociedade Brasileira De Pesquisa Odontologica, v. 28, n. 6, p. 469-477, 2014. 1806-8324 http://hdl.handle.net/11449/130035 10.1590/1807-3107BOR-2014.vol28.0054 S1806-83242014000100261 WOS:000352327500009 S1806-83242014000100261.pdf 8069912321732258 7008114923397947 5733639342016958 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242014000100261&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/130035 |
identifier_str_mv |
Brazilian Oral Research. Sao Paulo: Sociedade Brasileira De Pesquisa Odontologica, v. 28, n. 6, p. 469-477, 2014. 1806-8324 10.1590/1807-3107BOR-2014.vol28.0054 S1806-83242014000100261 WOS:000352327500009 S1806-83242014000100261.pdf 8069912321732258 7008114923397947 5733639342016958 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
469-477 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira De Pesquisa Odontologica |
publisher.none.fl_str_mv |
Sociedade Brasileira De Pesquisa Odontologica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965539735437312 |