Efeitos da terapia fotodinâmica na expressão dos genes SAP5, LIP9 e PLB2 em Candida albicans

• Autor(a) principal: Freire, Fernanda [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/108839
• topic: Biofilme; Candida albicans; Fotoquimioterapia

With the increasing number of strains of Candida ssp. resistant to antifungal agents, becomes important and necessary the accomplishment of researches that evaluate the effects of new therapeutic methods, like Photodynamic therapy (PDT) in virulence factors of that gender, as the production of the hydrolytic enzymes aspartyl-proteinase (SAP), lipase (LIP) and phospholipase (PL). Thus, the objective of this study was to verify the effects of the photodynamic therapy on biofilms of Candida albicans, evaluating their effects on the expression of the genes SAP5, LIP9 and PLB2 for the yeast. Clinical strains of Candida albicans (n=9) isolated from patients bearers of the virus HIV and a pattern strain ATCC 18804 were used. The quantification of gene expression was related to the production of hydrolytic enzymes (SAP5, LIP9 and PLB2) in clinical samples and the reference strain using the PCR assay in real time. For photodynamic therapy were used laser-aluminum-gallium arsenide low power (red visible, 660 nm) as light source and the methylene blue to 300 μM, as a photosensitizer. We were assessed four experimental groups for each strain: a) F+L+: sensitization with methylene blue and laser irradiation; b) F+L-: only treatment with methylene blue; c) F-L+: only laser irradiation and d) F-L-: control group, without sensitization with methylene blue and light absence. The results were analyzed by the software of the real-time equipment. The PDT decreased gene expression in 60% of samples for gene SAP5 and 50% of the samples decreased expression of LIP9 and PLB2. It can be concluded that PDT had an effect on the genes SAP5, LIP9 and PLB2 of C. albicans