Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992005000100010 http://hdl.handle.net/11449/211849 |
Resumo: | Rabies is a viral disease of mammals transmitted through the bite of a rabid animal. A frugivorous adult male bat, Artibeus lituratus, family Phyllostomidae, was diagnosed as positive to rabies by direct immunofluorescence (DIF) and mouse inoculation test (MIT) of the bat's brain, both performed at the School of Veterinary Medicine and Animal Husbandry - FMVZ, UNESP, Botucatu, São Paulo State, Brazil. The animal collided with the window of a commercial establishment in the urban area during the day. With regard to DIF, a high amount of Negri bodies of several sizes was observed in the brain. The spleen and right kidney presented some Negri bodies too. In relation to MIT, the mice presented paralysis in the 7th day, and died in the day after with several characteristic small bodies. The reverse transcriptase-polymerase chain reaction (RT-PCR), followed by hemi-nested RT-PCR (hnRT-PCR) resulted in an amplification of fragments from the bat's brain viral RNA, 432bp in RT-PCR, and 274bp in hnRT-PCR, confirming the diagnosis. Therefore, the hnRT-PCR and DIF have good sensitivity and specificity, providing and confirming the diagnosis of the clinical samples in a short period of time. |
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Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazilsoutheastern BrazilrabiesPhyllostomidaefrugivorous batRabies is a viral disease of mammals transmitted through the bite of a rabid animal. A frugivorous adult male bat, Artibeus lituratus, family Phyllostomidae, was diagnosed as positive to rabies by direct immunofluorescence (DIF) and mouse inoculation test (MIT) of the bat's brain, both performed at the School of Veterinary Medicine and Animal Husbandry - FMVZ, UNESP, Botucatu, São Paulo State, Brazil. The animal collided with the window of a commercial establishment in the urban area during the day. With regard to DIF, a high amount of Negri bodies of several sizes was observed in the brain. The spleen and right kidney presented some Negri bodies too. In relation to MIT, the mice presented paralysis in the 7th day, and died in the day after with several characteristic small bodies. The reverse transcriptase-polymerase chain reaction (RT-PCR), followed by hemi-nested RT-PCR (hnRT-PCR) resulted in an amplification of fragments from the bat's brain viral RNA, 432bp in RT-PCR, and 274bp in hnRT-PCR, confirming the diagnosis. Therefore, the hnRT-PCR and DIF have good sensitivity and specificity, providing and confirming the diagnosis of the clinical samples in a short period of time.Universidade Estadual Paulista, School of Veterinary Medicine and Animal HusbandryUniversidade Estadual Paulista, School of Veterinary Medicine and Animal HusbandryCentro de Estudos de Venenos e Animais PeçonhentosUniversidade Estadual Paulista (Unesp)Langoni, H. [UNESP]Lima, K. [UNESP]Menozzi, B. D. [UNESP]Silva, R. C. [UNESP]2021-07-14T10:30:35Z2021-07-14T10:30:35Z2005-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article84-87application/pdfhttp://dx.doi.org/10.1590/S1678-91992005000100010Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 1, p. 84-87, 2005.1678-9199http://hdl.handle.net/11449/21184910.1590/S1678-91992005000100010S1678-91992005000100010S1678-91992005000100010.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins including Tropical Diseasesinfo:eu-repo/semantics/openAccess2023-12-21T06:21:25Zoai:repositorio.unesp.br:11449/211849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-21T06:21:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
title |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
spellingShingle |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil Langoni, H. [UNESP] southeastern Brazil rabies Phyllostomidae frugivorous bat |
title_short |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
title_full |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
title_fullStr |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
title_full_unstemmed |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
title_sort |
Rabies in the big fruit-esting bat Artibeus lituratus from Botucatu, Southeastern Brazil |
author |
Langoni, H. [UNESP] |
author_facet |
Langoni, H. [UNESP] Lima, K. [UNESP] Menozzi, B. D. [UNESP] Silva, R. C. [UNESP] |
author_role |
author |
author2 |
Lima, K. [UNESP] Menozzi, B. D. [UNESP] Silva, R. C. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Langoni, H. [UNESP] Lima, K. [UNESP] Menozzi, B. D. [UNESP] Silva, R. C. [UNESP] |
dc.subject.por.fl_str_mv |
southeastern Brazil rabies Phyllostomidae frugivorous bat |
topic |
southeastern Brazil rabies Phyllostomidae frugivorous bat |
description |
Rabies is a viral disease of mammals transmitted through the bite of a rabid animal. A frugivorous adult male bat, Artibeus lituratus, family Phyllostomidae, was diagnosed as positive to rabies by direct immunofluorescence (DIF) and mouse inoculation test (MIT) of the bat's brain, both performed at the School of Veterinary Medicine and Animal Husbandry - FMVZ, UNESP, Botucatu, São Paulo State, Brazil. The animal collided with the window of a commercial establishment in the urban area during the day. With regard to DIF, a high amount of Negri bodies of several sizes was observed in the brain. The spleen and right kidney presented some Negri bodies too. In relation to MIT, the mice presented paralysis in the 7th day, and died in the day after with several characteristic small bodies. The reverse transcriptase-polymerase chain reaction (RT-PCR), followed by hemi-nested RT-PCR (hnRT-PCR) resulted in an amplification of fragments from the bat's brain viral RNA, 432bp in RT-PCR, and 274bp in hnRT-PCR, confirming the diagnosis. Therefore, the hnRT-PCR and DIF have good sensitivity and specificity, providing and confirming the diagnosis of the clinical samples in a short period of time. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-03 2021-07-14T10:30:35Z 2021-07-14T10:30:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992005000100010 Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 1, p. 84-87, 2005. 1678-9199 http://hdl.handle.net/11449/211849 10.1590/S1678-91992005000100010 S1678-91992005000100010 S1678-91992005000100010.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992005000100010 http://hdl.handle.net/11449/211849 |
identifier_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases. Botucatu, SP, Brazil: Centro de Estudos de Venenos e Animais Peçonhentos, v. 11, n. 1, p. 84-87, 2005. 1678-9199 10.1590/S1678-91992005000100010 S1678-91992005000100010 S1678-91992005000100010.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins including Tropical Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
84-87 application/pdf |
dc.publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
publisher.none.fl_str_mv |
Centro de Estudos de Venenos e Animais Peçonhentos |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797790066572001280 |