Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2017.04.002 http://hdl.handle.net/11449/178837 |
Resumo: | Background The antimicrobial photodynamic therapy (aPDT) inactivates the target cell via reactions among the photosensitizer (PS), Laser or Led and O2. The aim of this study was to evaluate the tissue reaction and cytokine production promoted by aPDT with curcumin photosensitizer. Methods Polyethylene tubes containing saline solution (control), 5% sodium hypochlorite (NaOCl) and aPDT with curcumin PS 500 mg/L, were implanted into dorsal connective tissue of Wistar rats. After 7, 15, 30, 60 and 90 days of implantation, the animals were euthanized and the tubes with surrounding tissues were removed. The specimens were divided in two part, one half was processed, fixed and prepared for histological analysis by staining with hematoxylin and eosin. The other half was collected for IL-1β and IL-6 cytokine production using ELISA assay. The results were statistically analyzed by Kruskal-Wallis test followed by Dunn test (p < 0.05) for tissue reaction and ANOVA followed by Bonferroni's correction (p < 0.05) for ELISA. Results All groups showed severe tissue reactions at 7 days, whilst a significantly decrease by time was observed. Regarding to cytokine production, aPDT increases the IL-1β levels in all periods of time (p < 0.05). However, for IL-6 levels, the highest value was observed with aPDT on the 90th day (p < 0.05). Conclusions aPDT with curcumin PS 500 mg/L demonstrated biocompatibility similar to saline solution and induced the IL-1β and IL-6 cytokines production. |
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Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapyAntimicrobial photodynamic therapyBiocompatibilityCytokineEndodontic treatmentRoot canal irrigantsBackground The antimicrobial photodynamic therapy (aPDT) inactivates the target cell via reactions among the photosensitizer (PS), Laser or Led and O2. The aim of this study was to evaluate the tissue reaction and cytokine production promoted by aPDT with curcumin photosensitizer. Methods Polyethylene tubes containing saline solution (control), 5% sodium hypochlorite (NaOCl) and aPDT with curcumin PS 500 mg/L, were implanted into dorsal connective tissue of Wistar rats. After 7, 15, 30, 60 and 90 days of implantation, the animals were euthanized and the tubes with surrounding tissues were removed. The specimens were divided in two part, one half was processed, fixed and prepared for histological analysis by staining with hematoxylin and eosin. The other half was collected for IL-1β and IL-6 cytokine production using ELISA assay. The results were statistically analyzed by Kruskal-Wallis test followed by Dunn test (p < 0.05) for tissue reaction and ANOVA followed by Bonferroni's correction (p < 0.05) for ELISA. Results All groups showed severe tissue reactions at 7 days, whilst a significantly decrease by time was observed. Regarding to cytokine production, aPDT increases the IL-1β levels in all periods of time (p < 0.05). However, for IL-6 levels, the highest value was observed with aPDT on the 90th day (p < 0.05). Conclusions aPDT with curcumin PS 500 mg/L demonstrated biocompatibility similar to saline solution and induced the IL-1β and IL-6 cytokines production.Department of Restorative Dentistry Discipline of EndodonticsDiscipline of Endodontics São Paulo State University (Unesp) School of DentistryOptics Group Physics Institute of São Carlos University of São PauloDepartment of Basic Science Discipline of PharmacologyDiscipline of Pharmacology São Paulo State University (Unesp) School of DentistryDepartment of Restorative Dentistry Discipline of EndodonticsDiscipline of Endodontics São Paulo State University (Unesp) School of DentistryDepartment of Basic Science Discipline of PharmacologyDiscipline of Pharmacology São Paulo State University (Unesp) School of DentistryUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Sivieri-Araujo, Gustavo [UNESP]Queiroz, Índia Olinta de Azevedo [UNESP]Dal Fabbro, Renan [UNESP]Esteves, Fernanda [UNESP]Cintra, Luciano Tavares Angelo [UNESP]Duarte, Paulo Carvalho Tobias [UNESP]Bagnato, Vanderlei SalvadorOliveira, Sandra Helena Penha de [UNESP]Gomes Filho, João Eduardo [UNESP]2018-12-11T17:32:18Z2018-12-11T17:32:18Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article315-318application/pdfhttp://dx.doi.org/10.1016/j.pdpdt.2017.04.002Photodiagnosis and Photodynamic Therapy, v. 18, p. 315-318.1873-15971572-1000http://hdl.handle.net/11449/17883710.1016/j.pdpdt.2017.04.0022-s2.0-850185856992-s2.0-85018585699.pdf9235743081667362Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapy0,647info:eu-repo/semantics/openAccess2023-10-18T06:04:17Zoai:repositorio.unesp.br:11449/178837Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-18T06:04:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
title |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
spellingShingle |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy Sivieri-Araujo, Gustavo [UNESP] Antimicrobial photodynamic therapy Biocompatibility Cytokine Endodontic treatment Root canal irrigants |
title_short |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
title_full |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
title_fullStr |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
title_full_unstemmed |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
title_sort |
Rat tissue reaction and cytokine production induced by antimicrobial photodynamic therapy |
author |
Sivieri-Araujo, Gustavo [UNESP] |
author_facet |
Sivieri-Araujo, Gustavo [UNESP] Queiroz, Índia Olinta de Azevedo [UNESP] Dal Fabbro, Renan [UNESP] Esteves, Fernanda [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Duarte, Paulo Carvalho Tobias [UNESP] Bagnato, Vanderlei Salvador Oliveira, Sandra Helena Penha de [UNESP] Gomes Filho, João Eduardo [UNESP] |
author_role |
author |
author2 |
Queiroz, Índia Olinta de Azevedo [UNESP] Dal Fabbro, Renan [UNESP] Esteves, Fernanda [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Duarte, Paulo Carvalho Tobias [UNESP] Bagnato, Vanderlei Salvador Oliveira, Sandra Helena Penha de [UNESP] Gomes Filho, João Eduardo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Sivieri-Araujo, Gustavo [UNESP] Queiroz, Índia Olinta de Azevedo [UNESP] Dal Fabbro, Renan [UNESP] Esteves, Fernanda [UNESP] Cintra, Luciano Tavares Angelo [UNESP] Duarte, Paulo Carvalho Tobias [UNESP] Bagnato, Vanderlei Salvador Oliveira, Sandra Helena Penha de [UNESP] Gomes Filho, João Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial photodynamic therapy Biocompatibility Cytokine Endodontic treatment Root canal irrigants |
topic |
Antimicrobial photodynamic therapy Biocompatibility Cytokine Endodontic treatment Root canal irrigants |
description |
Background The antimicrobial photodynamic therapy (aPDT) inactivates the target cell via reactions among the photosensitizer (PS), Laser or Led and O2. The aim of this study was to evaluate the tissue reaction and cytokine production promoted by aPDT with curcumin photosensitizer. Methods Polyethylene tubes containing saline solution (control), 5% sodium hypochlorite (NaOCl) and aPDT with curcumin PS 500 mg/L, were implanted into dorsal connective tissue of Wistar rats. After 7, 15, 30, 60 and 90 days of implantation, the animals were euthanized and the tubes with surrounding tissues were removed. The specimens were divided in two part, one half was processed, fixed and prepared for histological analysis by staining with hematoxylin and eosin. The other half was collected for IL-1β and IL-6 cytokine production using ELISA assay. The results were statistically analyzed by Kruskal-Wallis test followed by Dunn test (p < 0.05) for tissue reaction and ANOVA followed by Bonferroni's correction (p < 0.05) for ELISA. Results All groups showed severe tissue reactions at 7 days, whilst a significantly decrease by time was observed. Regarding to cytokine production, aPDT increases the IL-1β levels in all periods of time (p < 0.05). However, for IL-6 levels, the highest value was observed with aPDT on the 90th day (p < 0.05). Conclusions aPDT with curcumin PS 500 mg/L demonstrated biocompatibility similar to saline solution and induced the IL-1β and IL-6 cytokines production. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-01 2018-12-11T17:32:18Z 2018-12-11T17:32:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2017.04.002 Photodiagnosis and Photodynamic Therapy, v. 18, p. 315-318. 1873-1597 1572-1000 http://hdl.handle.net/11449/178837 10.1016/j.pdpdt.2017.04.002 2-s2.0-85018585699 2-s2.0-85018585699.pdf 9235743081667362 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2017.04.002 http://hdl.handle.net/11449/178837 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 18, p. 315-318. 1873-1597 1572-1000 10.1016/j.pdpdt.2017.04.002 2-s2.0-85018585699 2-s2.0-85018585699.pdf 9235743081667362 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy 0,647 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
315-318 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964603300446208 |