Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?pid=S0074-02762011000600002&script=sci_arttext http://hdl.handle.net/11449/3553 |
Resumo: | Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast. |
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Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungalsCandida parapsilosisCandida orthopsilosisBiofilmhaemodialysis solutionCandida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Análises ClínicasUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Fitossanidade VegetalUniversidade de Franca Laboratório de Pesquisa em Microbiologia AplicadaUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Análises ClínicasUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Fitossanidade VegetalPADC/FCFAr-UNESP: 2009/54Instituto Oswaldo Cruz, Ministério da SaúdeUniversidade Estadual Paulista (Unesp)Universidade de Franca Laboratório de Pesquisa em Microbiologia AplicadaPires, Regina Helena [UNESP]Santos, Jaime Maia dos [UNESP]Zaia, José EduardoMartins, Carlos Henrique GomesMendes-Giannini, Maria José Soares [UNESP]2014-05-20T13:16:52Z2014-05-20T13:16:52Z2011-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article646-654application/pdfhttp://www.scielo.br/scielo.php?pid=S0074-02762011000600002&script=sci_arttextMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 6, p. 646-654, 2011.0074-0276http://hdl.handle.net/11449/3553S0074-02762011000600002WOS:000296022400002S0074-02762011000600002.pdf65859378888947980000-0002-8059-0826SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-18T06:08:36Zoai:repositorio.unesp.br:11449/3553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-18T06:08:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
title |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
spellingShingle |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals Pires, Regina Helena [UNESP] Candida parapsilosis Candida orthopsilosis Biofilm haemodialysis solution |
title_short |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
title_full |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
title_fullStr |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
title_full_unstemmed |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
title_sort |
Candida parapsilosis complex water isolates from a haemodialysis unit: biofilm production and in vitro evaluation of the use of clinical antifungals |
author |
Pires, Regina Helena [UNESP] |
author_facet |
Pires, Regina Helena [UNESP] Santos, Jaime Maia dos [UNESP] Zaia, José Eduardo Martins, Carlos Henrique Gomes Mendes-Giannini, Maria José Soares [UNESP] |
author_role |
author |
author2 |
Santos, Jaime Maia dos [UNESP] Zaia, José Eduardo Martins, Carlos Henrique Gomes Mendes-Giannini, Maria José Soares [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Franca Laboratório de Pesquisa em Microbiologia Aplicada |
dc.contributor.author.fl_str_mv |
Pires, Regina Helena [UNESP] Santos, Jaime Maia dos [UNESP] Zaia, José Eduardo Martins, Carlos Henrique Gomes Mendes-Giannini, Maria José Soares [UNESP] |
dc.subject.por.fl_str_mv |
Candida parapsilosis Candida orthopsilosis Biofilm haemodialysis solution |
topic |
Candida parapsilosis Candida orthopsilosis Biofilm haemodialysis solution |
description |
Candida parapsilosis, currently divided into three distinct species, proliferates in glucose-rich solutions and has been associated with infections resulting from the use of medical devices made of plastic, an environment common in dialysis centres. The aims of this study were (i) to screen for Candida orthopsilosis and Candida metapsilosis (100 environmental isolates previously identified as C. parapsilosis), (ii) to test the ability of these isolates to form biofilm and (iii) to investigate the in vitro susceptibility of Candida spp biofilms to the antifungal agents, fluconazole (FLC) and amphotericin B (AMB). Isolates were obtained from a hydraulic circuit collected from a haemodialysis unit. Based on molecular criteria, 47 strains were re-identified as C. orthopsilosis and 53 as C. parapsilosis. Analyses using a formazan salt reduction assay and total viable count, together with microscopy studies, revealed that 72 strains were able to form biofilm that was structurally similar, but with minor differences in morphology. A microtitre-based colorimetric assay used to test the susceptibility of fungal biofilms to AMB and FLC demonstrated that the C. parapsilosis complex displayed an increased resistance to these antifungal agents. The results from these analyses may provide a basis for implementing quality controls and monitoring to ensure the microbiological purity of dialysis water, including the presence of yeast. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 2014-05-20T13:16:52Z 2014-05-20T13:16:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?pid=S0074-02762011000600002&script=sci_arttext Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 6, p. 646-654, 2011. 0074-0276 http://hdl.handle.net/11449/3553 S0074-02762011000600002 WOS:000296022400002 S0074-02762011000600002.pdf 6585937888894798 0000-0002-8059-0826 |
url |
http://www.scielo.br/scielo.php?pid=S0074-02762011000600002&script=sci_arttext http://hdl.handle.net/11449/3553 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 106, n. 6, p. 646-654, 2011. 0074-0276 S0074-02762011000600002 WOS:000296022400002 S0074-02762011000600002.pdf 6585937888894798 0000-0002-8059-0826 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
646-654 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964609410498560 |