Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing
Main Author: | |
---|---|
Publication Date: | 2022 |
Other Authors: | , , , , , , , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.ijbiomac.2022.05.025 http://hdl.handle.net/11449/241883 |
Summary: | Gold nanoparticles (AuNPs) have shown interesting properties and specific biofunctions, providing benefits and new opportunities for controlled release systems. In this research, we demonstrated the use of natural rubber latex (NRL) from Hevea brasiliensis as a carrier of AuNPs and the antibiotic metronidazole (MET). We prepared AuNP-MET-NRL and characterized by physicochemical, biological and in vitro release assays. The effect of AuNPs on MET release was evaluated using UV–Vis and Laser-Induced Breakdown Spectroscopy (LIBS) techniques. AuNPs synthesized by Turkevich and Frens method resulted in a spherical shape with diameters of 34.8 ± 5.5 nm. We verified that there was no emergence or disappearance of new vibrational bands. Qualitatively and quantitatively, we showed that the MET crystals dispersed throughout the NRL. The Young's modulus and elongation values at dressing rupture were in the range appropriate for human skin application. 64.70% of the AuNP-MET complex was released within 100 h, exhibiting a second-order exponential release profile. The LIBS technique allowed monitoring of the AuNP release, indicating the Au emission peak reduction at 267.57 nm over time. Moreover, the dressing displayed an excellent hemocompatibility and fibroblast cell viability. These results demonstrated that the AuNP-MET-NRL wound dressing is a promising approach for dermal applications. |
id |
UNSP_95edf8d0664680944d0a401ae8fc3e6d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/241883 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressingGold nanoparticlesLIBSSustained releaseGold nanoparticles (AuNPs) have shown interesting properties and specific biofunctions, providing benefits and new opportunities for controlled release systems. In this research, we demonstrated the use of natural rubber latex (NRL) from Hevea brasiliensis as a carrier of AuNPs and the antibiotic metronidazole (MET). We prepared AuNP-MET-NRL and characterized by physicochemical, biological and in vitro release assays. The effect of AuNPs on MET release was evaluated using UV–Vis and Laser-Induced Breakdown Spectroscopy (LIBS) techniques. AuNPs synthesized by Turkevich and Frens method resulted in a spherical shape with diameters of 34.8 ± 5.5 nm. We verified that there was no emergence or disappearance of new vibrational bands. Qualitatively and quantitatively, we showed that the MET crystals dispersed throughout the NRL. The Young's modulus and elongation values at dressing rupture were in the range appropriate for human skin application. 64.70% of the AuNP-MET complex was released within 100 h, exhibiting a second-order exponential release profile. The LIBS technique allowed monitoring of the AuNP release, indicating the Au emission peak reduction at 267.57 nm over time. Moreover, the dressing displayed an excellent hemocompatibility and fibroblast cell viability. These results demonstrated that the AuNP-MET-NRL wound dressing is a promising approach for dermal applications.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Bioengineering & Biomaterials Group School of Pharmaceutical Sciences, SPSão Paulo State University (UNESP) Post-Graduate Program in Biotechnology Institute of Chemistry, SPSão Paulo State University (UNESP) Department of Biotechnology School of Sciences Humanities and Languages, SPTerasaki Institute for Biomedical Innovation (TIBI), 11507 W Olympic Blvd.Escuela de Agronomía Facultad de Ciencias Agronómicas y de los Alimentos Pontificia Universidad Católica de ValparaísoFederal University of Mato Grosso do Sul (UFMS) Institute of Physics, MSBrazilian Agricultural Research Corporation (EMBRAPA), SPFederal University of Santa Catarina (UFSC) Department of Physics Center for Physical Sciences and Mathematics (CFM), SCArea of Exact Sciences and Engineering University of Caxias do Sul (UCS), RSSão Paulo University (USP) Engineering School of Lorena, SPSão Paulo State University (UNESP) Bioengineering & Biomaterials Group School of Pharmaceutical Sciences, SPSão Paulo State University (UNESP) Post-Graduate Program in Biotechnology Institute of Chemistry, SPSão Paulo State University (UNESP) Department of Biotechnology School of Sciences Humanities and Languages, SPFAPESP: 2011/17411-8FAPESP: 2012/12958-1FAPESP: 2014/17526-8FAPESP: 2017/19603-8FAPESP: 2020/08655-0CNPq: 317203/2021-5CNPq: 470261/2012-9Universidade Estadual Paulista (UNESP)Terasaki Institute for Biomedical Innovation (TIBI)Pontificia Universidad Católica de ValparaísoUniversidade Federal de Mato Grosso do Sul (UFMS)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal de Santa Catarina (UFSC)University of Caxias do Sul (UCS)Universidade de São Paulo (USP)Borges, Felipe Azevedo [UNESP]de Camargo Drago, Bruno [UNESP]Baggio, Luís Otávio [UNESP]de Barros, Natan RobertoSant'Ana Pegorin Brasil, Giovana [UNESP]Scontri, Mateus [UNESP]Mussagy, Cassamo Ussemaneda Silva Ribeiro, Matheus CíceroMilori, Débora Marcondes Bastos Pereirade Morais, Carla PereiraMarangoni, Bruno SpolonNicolodelli, GustavoMecwan, MarvinMandal, KalpanaGuerra, Nayrim BrizuelaMenegatti, Carlos RenatoHerculano, Rondinelli Donizetti [UNESP]2023-03-02T02:49:48Z2023-03-02T02:49:48Z2022-06-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article568-579http://dx.doi.org/10.1016/j.ijbiomac.2022.05.025International Journal of Biological Macromolecules, v. 211, p. 568-579.1879-00030141-8130http://hdl.handle.net/11449/24188310.1016/j.ijbiomac.2022.05.0252-s2.0-85130768653Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Biological Macromoleculesinfo:eu-repo/semantics/openAccess2023-03-02T02:49:48Zoai:repositorio.unesp.br:11449/241883Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T02:49:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
title |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
spellingShingle |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing Borges, Felipe Azevedo [UNESP] Gold nanoparticles LIBS Sustained release |
title_short |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
title_full |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
title_fullStr |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
title_full_unstemmed |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
title_sort |
Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing |
author |
Borges, Felipe Azevedo [UNESP] |
author_facet |
Borges, Felipe Azevedo [UNESP] de Camargo Drago, Bruno [UNESP] Baggio, Luís Otávio [UNESP] de Barros, Natan Roberto Sant'Ana Pegorin Brasil, Giovana [UNESP] Scontri, Mateus [UNESP] Mussagy, Cassamo Ussemane da Silva Ribeiro, Matheus Cícero Milori, Débora Marcondes Bastos Pereira de Morais, Carla Pereira Marangoni, Bruno Spolon Nicolodelli, Gustavo Mecwan, Marvin Mandal, Kalpana Guerra, Nayrim Brizuela Menegatti, Carlos Renato Herculano, Rondinelli Donizetti [UNESP] |
author_role |
author |
author2 |
de Camargo Drago, Bruno [UNESP] Baggio, Luís Otávio [UNESP] de Barros, Natan Roberto Sant'Ana Pegorin Brasil, Giovana [UNESP] Scontri, Mateus [UNESP] Mussagy, Cassamo Ussemane da Silva Ribeiro, Matheus Cícero Milori, Débora Marcondes Bastos Pereira de Morais, Carla Pereira Marangoni, Bruno Spolon Nicolodelli, Gustavo Mecwan, Marvin Mandal, Kalpana Guerra, Nayrim Brizuela Menegatti, Carlos Renato Herculano, Rondinelli Donizetti [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Terasaki Institute for Biomedical Innovation (TIBI) Pontificia Universidad Católica de Valparaíso Universidade Federal de Mato Grosso do Sul (UFMS) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Federal de Santa Catarina (UFSC) University of Caxias do Sul (UCS) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Borges, Felipe Azevedo [UNESP] de Camargo Drago, Bruno [UNESP] Baggio, Luís Otávio [UNESP] de Barros, Natan Roberto Sant'Ana Pegorin Brasil, Giovana [UNESP] Scontri, Mateus [UNESP] Mussagy, Cassamo Ussemane da Silva Ribeiro, Matheus Cícero Milori, Débora Marcondes Bastos Pereira de Morais, Carla Pereira Marangoni, Bruno Spolon Nicolodelli, Gustavo Mecwan, Marvin Mandal, Kalpana Guerra, Nayrim Brizuela Menegatti, Carlos Renato Herculano, Rondinelli Donizetti [UNESP] |
dc.subject.por.fl_str_mv |
Gold nanoparticles LIBS Sustained release |
topic |
Gold nanoparticles LIBS Sustained release |
description |
Gold nanoparticles (AuNPs) have shown interesting properties and specific biofunctions, providing benefits and new opportunities for controlled release systems. In this research, we demonstrated the use of natural rubber latex (NRL) from Hevea brasiliensis as a carrier of AuNPs and the antibiotic metronidazole (MET). We prepared AuNP-MET-NRL and characterized by physicochemical, biological and in vitro release assays. The effect of AuNPs on MET release was evaluated using UV–Vis and Laser-Induced Breakdown Spectroscopy (LIBS) techniques. AuNPs synthesized by Turkevich and Frens method resulted in a spherical shape with diameters of 34.8 ± 5.5 nm. We verified that there was no emergence or disappearance of new vibrational bands. Qualitatively and quantitatively, we showed that the MET crystals dispersed throughout the NRL. The Young's modulus and elongation values at dressing rupture were in the range appropriate for human skin application. 64.70% of the AuNP-MET complex was released within 100 h, exhibiting a second-order exponential release profile. The LIBS technique allowed monitoring of the AuNP release, indicating the Au emission peak reduction at 267.57 nm over time. Moreover, the dressing displayed an excellent hemocompatibility and fibroblast cell viability. These results demonstrated that the AuNP-MET-NRL wound dressing is a promising approach for dermal applications. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-06-30 2023-03-02T02:49:48Z 2023-03-02T02:49:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijbiomac.2022.05.025 International Journal of Biological Macromolecules, v. 211, p. 568-579. 1879-0003 0141-8130 http://hdl.handle.net/11449/241883 10.1016/j.ijbiomac.2022.05.025 2-s2.0-85130768653 |
url |
http://dx.doi.org/10.1016/j.ijbiomac.2022.05.025 http://hdl.handle.net/11449/241883 |
identifier_str_mv |
International Journal of Biological Macromolecules, v. 211, p. 568-579. 1879-0003 0141-8130 10.1016/j.ijbiomac.2022.05.025 2-s2.0-85130768653 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Biological Macromolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
568-579 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790409389244416 |