Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death
Main Author: | |
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Publication Date: | 2017 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.anireprosci.2017.10.001 http://hdl.handle.net/11449/175312 |
Summary: | The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA). A hundred ewes were inseminated with thawed spermatozoa and gestation rate was correlated with the proportions of each subpopulation using Pearson correlation matrix and linear regression. Three distinct subpopulations were identified in the thawed samples of either ovine ejaculate collected in artificial vaginas (AV) or ovine spermatozoa retrieved from the cauda epididymis. Subpopulation 1 (SP1) was characterized by spermatozoa with slow and non-linear motion, subpopulation 2 (SP2) was classified as hyperactived spermatozoa and subpopulation 3 (SP3) was composed of spermatozoa with fast, linear motion. The largest subpopulation in all groups was SP1. The semen collected in an artificial vagina had a higher (P < 0.05) percentage of SP2 and lower (P <0.05) percentage of SP1 when compared to spermatozoa recovered after death. Increasing time of storage after death had a detrimental effect on sperm samples, increasing (P < 0.05) the percentage of SP1 and decreasing (P < 0.05) SP2. Length of storage after death was the only variable that influenced, with an inversely proportional relationship, SP3. In samples stored for 48 h after death no SP3 spermatozoa were present. The addition of seminal plasma to the cryopreservative decreased (P < 0.05) the subpopulation of hyperactived spermatozoa (SP2). We conclude that, after thawing there are three sperm subpopulations in the spermatozoa obtained from the cauda epididymides and the semen collected in AVs and that the relative proportions of these subpopulations varies with the time of storage post mortem and the presence of 20% of seminal plasma in the extender. However, we conclude that these subpopulations do not correlate with fertility after intrauterine artificial insemination. |
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Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after deathMotion patternRamSeminal plasmaThe objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA). A hundred ewes were inseminated with thawed spermatozoa and gestation rate was correlated with the proportions of each subpopulation using Pearson correlation matrix and linear regression. Three distinct subpopulations were identified in the thawed samples of either ovine ejaculate collected in artificial vaginas (AV) or ovine spermatozoa retrieved from the cauda epididymis. Subpopulation 1 (SP1) was characterized by spermatozoa with slow and non-linear motion, subpopulation 2 (SP2) was classified as hyperactived spermatozoa and subpopulation 3 (SP3) was composed of spermatozoa with fast, linear motion. The largest subpopulation in all groups was SP1. The semen collected in an artificial vagina had a higher (P < 0.05) percentage of SP2 and lower (P <0.05) percentage of SP1 when compared to spermatozoa recovered after death. Increasing time of storage after death had a detrimental effect on sperm samples, increasing (P < 0.05) the percentage of SP1 and decreasing (P < 0.05) SP2. Length of storage after death was the only variable that influenced, with an inversely proportional relationship, SP3. In samples stored for 48 h after death no SP3 spermatozoa were present. The addition of seminal plasma to the cryopreservative decreased (P < 0.05) the subpopulation of hyperactived spermatozoa (SP2). We conclude that, after thawing there are three sperm subpopulations in the spermatozoa obtained from the cauda epididymides and the semen collected in AVs and that the relative proportions of these subpopulations varies with the time of storage post mortem and the presence of 20% of seminal plasma in the extender. However, we conclude that these subpopulations do not correlate with fertility after intrauterine artificial insemination.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Technology Postgraduate studies in Bioprocess Engineering and Biotechnology Human and Animal Health Federal University of Paraná, Rua dos FuncionáriosDepartment of Veterinary Medicine Postgraduate Studies in Animal Science Pontifical Catholic University of Paraná, Rua Imaculada Conceição, Prado VelhoDepartment of Animal Reproduction and Veterinary Radiology-FMVZ − UNESP, Distrito de Rubiao Júnior, s/nDepartment of Animal Reproduction and Veterinary Radiology-FMVZ − UNESP, Distrito de Rubiao Júnior, s/nFederal University of ParanáPontifical Catholic University of ParanáUniversidade Estadual Paulista (Unesp)Bergstein-Galan, Tácia GomesWeiss, Romildo RomualdoKozicki, Luiz ErnandesBicudo, Sony Dimas [UNESP]2018-12-11T17:15:15Z2018-12-11T17:15:15Z2017-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20-27application/pdfhttp://dx.doi.org/10.1016/j.anireprosci.2017.10.001Animal Reproduction Science, v. 187, p. 20-27.0378-4320http://hdl.handle.net/11449/17531210.1016/j.anireprosci.2017.10.0012-s2.0-850307926202-s2.0-85030792620.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Science0,704info:eu-repo/semantics/openAccess2023-10-14T06:09:53Zoai:repositorio.unesp.br:11449/175312Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:09:53Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
title |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
spellingShingle |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death Bergstein-Galan, Tácia Gomes Motion pattern Ram Seminal plasma |
title_short |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
title_full |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
title_fullStr |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
title_full_unstemmed |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
title_sort |
Sperm subpopulations in ejaculated sperm and spermatozoa recovered from ovine epididymides up to 48 h after death |
author |
Bergstein-Galan, Tácia Gomes |
author_facet |
Bergstein-Galan, Tácia Gomes Weiss, Romildo Romualdo Kozicki, Luiz Ernandes Bicudo, Sony Dimas [UNESP] |
author_role |
author |
author2 |
Weiss, Romildo Romualdo Kozicki, Luiz Ernandes Bicudo, Sony Dimas [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Federal University of Paraná Pontifical Catholic University of Paraná Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bergstein-Galan, Tácia Gomes Weiss, Romildo Romualdo Kozicki, Luiz Ernandes Bicudo, Sony Dimas [UNESP] |
dc.subject.por.fl_str_mv |
Motion pattern Ram Seminal plasma |
topic |
Motion pattern Ram Seminal plasma |
description |
The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA). A hundred ewes were inseminated with thawed spermatozoa and gestation rate was correlated with the proportions of each subpopulation using Pearson correlation matrix and linear regression. Three distinct subpopulations were identified in the thawed samples of either ovine ejaculate collected in artificial vaginas (AV) or ovine spermatozoa retrieved from the cauda epididymis. Subpopulation 1 (SP1) was characterized by spermatozoa with slow and non-linear motion, subpopulation 2 (SP2) was classified as hyperactived spermatozoa and subpopulation 3 (SP3) was composed of spermatozoa with fast, linear motion. The largest subpopulation in all groups was SP1. The semen collected in an artificial vagina had a higher (P < 0.05) percentage of SP2 and lower (P <0.05) percentage of SP1 when compared to spermatozoa recovered after death. Increasing time of storage after death had a detrimental effect on sperm samples, increasing (P < 0.05) the percentage of SP1 and decreasing (P < 0.05) SP2. Length of storage after death was the only variable that influenced, with an inversely proportional relationship, SP3. In samples stored for 48 h after death no SP3 spermatozoa were present. The addition of seminal plasma to the cryopreservative decreased (P < 0.05) the subpopulation of hyperactived spermatozoa (SP2). We conclude that, after thawing there are three sperm subpopulations in the spermatozoa obtained from the cauda epididymides and the semen collected in AVs and that the relative proportions of these subpopulations varies with the time of storage post mortem and the presence of 20% of seminal plasma in the extender. However, we conclude that these subpopulations do not correlate with fertility after intrauterine artificial insemination. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-01 2018-12-11T17:15:15Z 2018-12-11T17:15:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2017.10.001 Animal Reproduction Science, v. 187, p. 20-27. 0378-4320 http://hdl.handle.net/11449/175312 10.1016/j.anireprosci.2017.10.001 2-s2.0-85030792620 2-s2.0-85030792620.pdf |
url |
http://dx.doi.org/10.1016/j.anireprosci.2017.10.001 http://hdl.handle.net/11449/175312 |
identifier_str_mv |
Animal Reproduction Science, v. 187, p. 20-27. 0378-4320 10.1016/j.anireprosci.2017.10.001 2-s2.0-85030792620 2-s2.0-85030792620.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science 0,704 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
20-27 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789396563394560 |