Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/iej.13929 http://hdl.handle.net/11449/247415 |
Resumo: | Aim: This study aimed to evaluate the cytotoxicity, biocompatibility and osteoinductive profile of a mineral trioxide aggregate (MTA)-hydrogel-based material (MTA Flow) in comparison with MTA Angelus. Methodology: Cell viability was evaluated in human periodontal ligament stem cells (hPDLSCs) using the methyl-thiazol-tetrazolium (MTT) colourimetric assay. Polyethylene tubes containing the tested materials and empty polyethylene tubes (control) were implanted in the subcutaneous tissue of Wistar rats. Cellular (lymphocyte infiltration) and extracellular events (ECM; collagen fibres) were analysed in histological sections. Immunohistochemical (collagen I, osteopontin, bone sialoprotein, bone morphogenetic protein4) analyses were also performed. Results: At 24, 48 and 72 h, all tested groups showed cell viability similar to control (p >.05). Regarding biocompatibility, all groups showed similar cellular events represented by a slight inflammatory reaction characterized by hyperaemia and a mild lymphocytic inflammatory infiltrate. The analysis of lymphocytes during the time showed a decrease in these cells in the control group and a significant interaction between MTA Angelus and control (p <.001), with MTA Angelus showing a more extensive inflammatory infiltrate. Regarding fibres, an increase in content was observed in all groups during the experimental time (7, 30 and 60 days), however, no difference was detected among the experimental groups (p =.063). After 60 days, the immunoexpression of bone matrix proteins in the MTA Flow group was similar to or higher than that observed in the MTA Angelus and in the control group. Conclusions: MTA Flow showed a non-cytotoxic behaviour, biocompatibility and ability to stimulate tissue mineralization. |
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Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal studyanimal modelcytotoxicityendodonticsmineral trioxide aggregateAim: This study aimed to evaluate the cytotoxicity, biocompatibility and osteoinductive profile of a mineral trioxide aggregate (MTA)-hydrogel-based material (MTA Flow) in comparison with MTA Angelus. Methodology: Cell viability was evaluated in human periodontal ligament stem cells (hPDLSCs) using the methyl-thiazol-tetrazolium (MTT) colourimetric assay. Polyethylene tubes containing the tested materials and empty polyethylene tubes (control) were implanted in the subcutaneous tissue of Wistar rats. Cellular (lymphocyte infiltration) and extracellular events (ECM; collagen fibres) were analysed in histological sections. Immunohistochemical (collagen I, osteopontin, bone sialoprotein, bone morphogenetic protein4) analyses were also performed. Results: At 24, 48 and 72 h, all tested groups showed cell viability similar to control (p >.05). Regarding biocompatibility, all groups showed similar cellular events represented by a slight inflammatory reaction characterized by hyperaemia and a mild lymphocytic inflammatory infiltrate. The analysis of lymphocytes during the time showed a decrease in these cells in the control group and a significant interaction between MTA Angelus and control (p <.001), with MTA Angelus showing a more extensive inflammatory infiltrate. Regarding fibres, an increase in content was observed in all groups during the experimental time (7, 30 and 60 days), however, no difference was detected among the experimental groups (p =.063). After 60 days, the immunoexpression of bone matrix proteins in the MTA Flow group was similar to or higher than that observed in the MTA Angelus and in the control group. Conclusions: MTA Flow showed a non-cytotoxic behaviour, biocompatibility and ability to stimulate tissue mineralization.Oral Pathology Department School of Dentistry Universidade Federal do Rio Grande do Sul (UFRGS)Department of Experimental Pathology Hospital de Clínicas de Porto Alegre (HCPA)Graduate Program in Dentistry/Endodontics School of Dentistry Universidade Federal do Rio Grande do Sul (UFRGS)Department of Stomatology A.C.Camargo Cancer CenterDepartment of Dentistry School of Dentistry Universidade de São Paulo (FOUSP)Master and Doctorate in Endodontics Universidade Estadual Paulista (UNESP)Molecular Pathology Area School of Dentistry Universidad de la República (UDELAR)Master and Doctorate in Endodontics Universidade Estadual Paulista (UNESP)Universidade Federal do Rio Grande do Sul (UFRGS)Hospital de Clínicas de Porto Alegre (HCPA)A.C.Camargo Cancer CenterUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidad de la República (UDELAR)Martins, Manoela DominguesCoelho, Fernanda HackMoreira, Maria StellaMarques, Márcia MartinsPilar, Emily Ferreira SallesPalo, Renato Miotto [UNESP]Silveira, Felipe MartinsKopper, Patrícia Maria Poli2023-07-29T13:15:29Z2023-07-29T13:15:29Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/iej.13929International Endodontic Journal.1365-25910143-2885http://hdl.handle.net/11449/24741510.1111/iej.139292-s2.0-85159906936Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Endodontic Journalinfo:eu-repo/semantics/openAccess2023-07-29T13:15:29Zoai:repositorio.unesp.br:11449/247415Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:15:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
title |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
spellingShingle |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study Martins, Manoela Domingues animal model cytotoxicity endodontics mineral trioxide aggregate |
title_short |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
title_full |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
title_fullStr |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
title_full_unstemmed |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
title_sort |
Cytotoxicity, biocompatibility and osteoinductive profile of an MTA-hydrogel-based cement: An in vitro and animal study |
author |
Martins, Manoela Domingues |
author_facet |
Martins, Manoela Domingues Coelho, Fernanda Hack Moreira, Maria Stella Marques, Márcia Martins Pilar, Emily Ferreira Salles Palo, Renato Miotto [UNESP] Silveira, Felipe Martins Kopper, Patrícia Maria Poli |
author_role |
author |
author2 |
Coelho, Fernanda Hack Moreira, Maria Stella Marques, Márcia Martins Pilar, Emily Ferreira Salles Palo, Renato Miotto [UNESP] Silveira, Felipe Martins Kopper, Patrícia Maria Poli |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio Grande do Sul (UFRGS) Hospital de Clínicas de Porto Alegre (HCPA) A.C.Camargo Cancer Center Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidad de la República (UDELAR) |
dc.contributor.author.fl_str_mv |
Martins, Manoela Domingues Coelho, Fernanda Hack Moreira, Maria Stella Marques, Márcia Martins Pilar, Emily Ferreira Salles Palo, Renato Miotto [UNESP] Silveira, Felipe Martins Kopper, Patrícia Maria Poli |
dc.subject.por.fl_str_mv |
animal model cytotoxicity endodontics mineral trioxide aggregate |
topic |
animal model cytotoxicity endodontics mineral trioxide aggregate |
description |
Aim: This study aimed to evaluate the cytotoxicity, biocompatibility and osteoinductive profile of a mineral trioxide aggregate (MTA)-hydrogel-based material (MTA Flow) in comparison with MTA Angelus. Methodology: Cell viability was evaluated in human periodontal ligament stem cells (hPDLSCs) using the methyl-thiazol-tetrazolium (MTT) colourimetric assay. Polyethylene tubes containing the tested materials and empty polyethylene tubes (control) were implanted in the subcutaneous tissue of Wistar rats. Cellular (lymphocyte infiltration) and extracellular events (ECM; collagen fibres) were analysed in histological sections. Immunohistochemical (collagen I, osteopontin, bone sialoprotein, bone morphogenetic protein4) analyses were also performed. Results: At 24, 48 and 72 h, all tested groups showed cell viability similar to control (p >.05). Regarding biocompatibility, all groups showed similar cellular events represented by a slight inflammatory reaction characterized by hyperaemia and a mild lymphocytic inflammatory infiltrate. The analysis of lymphocytes during the time showed a decrease in these cells in the control group and a significant interaction between MTA Angelus and control (p <.001), with MTA Angelus showing a more extensive inflammatory infiltrate. Regarding fibres, an increase in content was observed in all groups during the experimental time (7, 30 and 60 days), however, no difference was detected among the experimental groups (p =.063). After 60 days, the immunoexpression of bone matrix proteins in the MTA Flow group was similar to or higher than that observed in the MTA Angelus and in the control group. Conclusions: MTA Flow showed a non-cytotoxic behaviour, biocompatibility and ability to stimulate tissue mineralization. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:15:29Z 2023-07-29T13:15:29Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/iej.13929 International Endodontic Journal. 1365-2591 0143-2885 http://hdl.handle.net/11449/247415 10.1111/iej.13929 2-s2.0-85159906936 |
url |
http://dx.doi.org/10.1111/iej.13929 http://hdl.handle.net/11449/247415 |
identifier_str_mv |
International Endodontic Journal. 1365-2591 0143-2885 10.1111/iej.13929 2-s2.0-85159906936 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Endodontic Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789792753156096 |