Response of pulp cells to resin infiltration of enamel white spot-like lesions
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.dental.2021.01.014 http://hdl.handle.net/11449/208396 |
Resumo: | Objectives: To investigate the trans-enamel and trans-dentinal biological effects of treating enamel white spot-like lesions (EWSLs) with resin infiltration components (RICs) on odontoblast-like cells (MDPC-23) and human dental pulp cells (HDPCs). Methods: EWSLs were induced in 60 enamel/dentin discs (4.0 ± 0.2 mm thick) using S. mutans. The discs were adapted into artificial pulp chambers and MDPC-23 were seeded on the dentin surface. The components of a resin infiltration system (Icon) were applied individually or in combination on the enamel surface as following (n = 10/treatment): Etch, Infiltrant, Etch+Infiltrant, or Etch+Dry+Infiltrant. The application of water or hydrogen peroxide served as negative and positive controls, respectively. After 72 h, MDPC-23 viability was evaluated. The extracts were exposed for 72 h to pre-cultured MDPC-23 and HDPCs in 96-well plates to evaluate cell viability, alkaline phosphatase activity (ALP), mineralized nodule formation (MN), and the expression of inflammatory cytokines (ICs) and mineralization-related genes (MRs). Data were analyzed by ANOVA complemented with Tukey or Games-Howell post-hocs (α = 5%). Results: Cell viability, ALP activity, and MN formation were significantly reduced in response to the RICs, presenting intermediate values compared to positive and negative controls. Likewise, ICs were upregulated, whereas MRs were downregulated. Among the RICs, the Etch component caused the most notorious detrimental effects. Significance: Resin infiltration of EWSLs negatively affected the metabolism of pulp cells in vitro. Therefore, even though resin infiltration is a micro-invasive therapy for non-cavitated caries in enamel, it should be closely followed up seen that components may diffuse and unbalance pulp homeostasis. |
id |
UNSP_99395872b38bf216222e267a724a91df |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/208396 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Response of pulp cells to resin infiltration of enamel white spot-like lesionsCell viabilityCytotoxicityDental cariesDental enamelDental pulpHydrochloric acidResin infiltrationTEGDMAObjectives: To investigate the trans-enamel and trans-dentinal biological effects of treating enamel white spot-like lesions (EWSLs) with resin infiltration components (RICs) on odontoblast-like cells (MDPC-23) and human dental pulp cells (HDPCs). Methods: EWSLs were induced in 60 enamel/dentin discs (4.0 ± 0.2 mm thick) using S. mutans. The discs were adapted into artificial pulp chambers and MDPC-23 were seeded on the dentin surface. The components of a resin infiltration system (Icon) were applied individually or in combination on the enamel surface as following (n = 10/treatment): Etch, Infiltrant, Etch+Infiltrant, or Etch+Dry+Infiltrant. The application of water or hydrogen peroxide served as negative and positive controls, respectively. After 72 h, MDPC-23 viability was evaluated. The extracts were exposed for 72 h to pre-cultured MDPC-23 and HDPCs in 96-well plates to evaluate cell viability, alkaline phosphatase activity (ALP), mineralized nodule formation (MN), and the expression of inflammatory cytokines (ICs) and mineralization-related genes (MRs). Data were analyzed by ANOVA complemented with Tukey or Games-Howell post-hocs (α = 5%). Results: Cell viability, ALP activity, and MN formation were significantly reduced in response to the RICs, presenting intermediate values compared to positive and negative controls. Likewise, ICs were upregulated, whereas MRs were downregulated. Among the RICs, the Etch component caused the most notorious detrimental effects. Significance: Resin infiltration of EWSLs negatively affected the metabolism of pulp cells in vitro. Therefore, even though resin infiltration is a micro-invasive therapy for non-cavitated caries in enamel, it should be closely followed up seen that components may diffuse and unbalance pulp homeostasis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Dentistry State University of Maringá (UEM) School of DentistryDepartment of Operative Dentistry Endodontics and Dental Materials São Paulo University (USP) Bauru Faculty of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) School of DentistryDepartment of Dental Materials and Prosthodontics São Paulo State University (UNESP) School of DentistryDepartment of Morphology Genetics Orthodontics and Pediatric Dentistry São Paulo State University (UNESP) School of DentistryDepartment of Physiology and Pathology São Paulo State University (UNESP) School of DentistryFAPESP: 2018/04867-2CNPq: 427256/2016-0Universidade Estadual Paulista (Unesp)Universidade Estadual de Maringá (UEM)Universidade de São Paulo (USP)Mendes Soares, Igor Paulino [UNESP]Anovazzi, Giovana [UNESP]Anselmi, Caroline [UNESP]Leite, Maria Luísa [UNESP]Scheffel, Débora Lopes SallesSoares, Diana Gabrielade Souza Costa, Carlos Alberto [UNESP]Hebling, Josimeri [UNESP]2021-06-25T11:11:30Z2021-06-25T11:11:30Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.dental.2021.01.014Dental Materials.0109-5641http://hdl.handle.net/11449/20839610.1016/j.dental.2021.01.0142-s2.0-85100704800Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDental Materialsinfo:eu-repo/semantics/openAccess2021-10-23T19:02:10Zoai:repositorio.unesp.br:11449/208396Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
title |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
spellingShingle |
Response of pulp cells to resin infiltration of enamel white spot-like lesions Mendes Soares, Igor Paulino [UNESP] Cell viability Cytotoxicity Dental caries Dental enamel Dental pulp Hydrochloric acid Resin infiltration TEGDMA |
title_short |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
title_full |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
title_fullStr |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
title_full_unstemmed |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
title_sort |
Response of pulp cells to resin infiltration of enamel white spot-like lesions |
author |
Mendes Soares, Igor Paulino [UNESP] |
author_facet |
Mendes Soares, Igor Paulino [UNESP] Anovazzi, Giovana [UNESP] Anselmi, Caroline [UNESP] Leite, Maria Luísa [UNESP] Scheffel, Débora Lopes Salles Soares, Diana Gabriela de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author_role |
author |
author2 |
Anovazzi, Giovana [UNESP] Anselmi, Caroline [UNESP] Leite, Maria Luísa [UNESP] Scheffel, Débora Lopes Salles Soares, Diana Gabriela de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Maringá (UEM) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Mendes Soares, Igor Paulino [UNESP] Anovazzi, Giovana [UNESP] Anselmi, Caroline [UNESP] Leite, Maria Luísa [UNESP] Scheffel, Débora Lopes Salles Soares, Diana Gabriela de Souza Costa, Carlos Alberto [UNESP] Hebling, Josimeri [UNESP] |
dc.subject.por.fl_str_mv |
Cell viability Cytotoxicity Dental caries Dental enamel Dental pulp Hydrochloric acid Resin infiltration TEGDMA |
topic |
Cell viability Cytotoxicity Dental caries Dental enamel Dental pulp Hydrochloric acid Resin infiltration TEGDMA |
description |
Objectives: To investigate the trans-enamel and trans-dentinal biological effects of treating enamel white spot-like lesions (EWSLs) with resin infiltration components (RICs) on odontoblast-like cells (MDPC-23) and human dental pulp cells (HDPCs). Methods: EWSLs were induced in 60 enamel/dentin discs (4.0 ± 0.2 mm thick) using S. mutans. The discs were adapted into artificial pulp chambers and MDPC-23 were seeded on the dentin surface. The components of a resin infiltration system (Icon) were applied individually or in combination on the enamel surface as following (n = 10/treatment): Etch, Infiltrant, Etch+Infiltrant, or Etch+Dry+Infiltrant. The application of water or hydrogen peroxide served as negative and positive controls, respectively. After 72 h, MDPC-23 viability was evaluated. The extracts were exposed for 72 h to pre-cultured MDPC-23 and HDPCs in 96-well plates to evaluate cell viability, alkaline phosphatase activity (ALP), mineralized nodule formation (MN), and the expression of inflammatory cytokines (ICs) and mineralization-related genes (MRs). Data were analyzed by ANOVA complemented with Tukey or Games-Howell post-hocs (α = 5%). Results: Cell viability, ALP activity, and MN formation were significantly reduced in response to the RICs, presenting intermediate values compared to positive and negative controls. Likewise, ICs were upregulated, whereas MRs were downregulated. Among the RICs, the Etch component caused the most notorious detrimental effects. Significance: Resin infiltration of EWSLs negatively affected the metabolism of pulp cells in vitro. Therefore, even though resin infiltration is a micro-invasive therapy for non-cavitated caries in enamel, it should be closely followed up seen that components may diffuse and unbalance pulp homeostasis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:11:30Z 2021-06-25T11:11:30Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.dental.2021.01.014 Dental Materials. 0109-5641 http://hdl.handle.net/11449/208396 10.1016/j.dental.2021.01.014 2-s2.0-85100704800 |
url |
http://dx.doi.org/10.1016/j.dental.2021.01.014 http://hdl.handle.net/11449/208396 |
identifier_str_mv |
Dental Materials. 0109-5641 10.1016/j.dental.2021.01.014 2-s2.0-85100704800 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Dental Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790346890969088 |