Effect of processing methods on the cytotoxicity of methyl methacrylate-based ocular prostheses: An in vitro study

• Autor(a) principal: da Silva, Emily Vivianne Freitas [UNESP]
• Data de Publicação: 2021
• Outros Autores: Goiato, Marcelo Coelho [UNESP], Bitencourt, Sandro Basso [UNESP], Finer, Yoav, Brito, Victor Gustavo Balera, Takamiya, Aline Satie [UNESP], de Oliveira, Sandra Helena Penha [UNESP], dos Santos, Daniela Micheline [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.tiv.2021.105211; http://hdl.handle.net/11449/229110
• topic: Cellular immune response; Cytotoxicity; Methyl methacrylate; Ocular prosthesis

The study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 °C or 100 °C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP, room temperature for 20 min ± 60 °C for 30 min). Degree of conversion (DC), cytotoxicity, level of inflammatory mediators, gene expression of different markers, and apoptosis were evaluated. Data were submitted to ANOVA and Tukey test (p < 0.05). WB with longer processing time at higher temperature had highest DC (85.6%) and higher TGF β1-gene expression (1.39); long cycle low power MW showed lowest DC (69.6%), lower cell proliferation (85.4%, MTT), and large IL-2 release (39,297 ng/mL). AP with additional processing time showed lower cell proliferation (75.3%, Alamar Blue), and AP polymerized at room temperature showed higher CASP 9-gene expression (1.21). AP methods showed higher IL-6 release (>277 pg/mL). Short cycle medium power MW had higher IL-23 release (534.2 pg/mL). MW (long and short cycles) and AP polymerizations have triggered a more intense inflammatory response. Among methods recommended by the manufacturer, WB showed high DC and less cytotoxicity.