Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.intimp.2022.108807 http://hdl.handle.net/11449/240972 |
Resumo: | Preeclampsia (PE) is a multifactorial disease that is characterized by inflammation. Some of the factors responsible for this inflammation are the cells of the innate and adaptive immune systems and their interactions. The use of natural products, such as silibinin (SB), can contribute to the control of this inflammation and gestational success. The present study evaluated whether the flavonoid SB has an in vitro immunomodulatory effect on the signal transducers and transcription activators (STATs) signaling pathway and transcription factors of CD4+ T cell subsets obtained from preeclamptic and normotensive (NT) pregnant women. Peripheral blood mononuclear cells (PBMCs) from 18 preeclamptic and 18 NT pregnant women were cultured with and without SB to analyze the expression of STATs and transcription factors by flow cytometry, and cytokines were measured in the culture supernatant by ELISA. The results showed that treating cells with SB decreased STAT1/ STAT4/T-bet and STAT3/RORγt, which characteristic of Th1 and Th17 inflammatory profiles, as well as increased STAT6/GATA-3 and STAT5/FoxP3 of anti-inflammatory and regulatory profiles, respectively. In addition, PBMCs from preeclamptic women treated with SB released lower concentrations of inflammatory cytokines and higher levels of IL-10 and TGF-β. Therefore, SB plays an immunomodulatory role on CD4+ T cell subsets in PE, leading to the downregulation of inflammatory profiles and upregulation of anti-inflammatory and regulatory profiles. More studies are necessary to better understand the modulation of CD4+ T cell subsets by the JAK/STAT and NF-κB pathways in this gestational pathology. |
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Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsiaPreeclampsiaSilibininSTATsT cell subsetsTranscription factorsPreeclampsia (PE) is a multifactorial disease that is characterized by inflammation. Some of the factors responsible for this inflammation are the cells of the innate and adaptive immune systems and their interactions. The use of natural products, such as silibinin (SB), can contribute to the control of this inflammation and gestational success. The present study evaluated whether the flavonoid SB has an in vitro immunomodulatory effect on the signal transducers and transcription activators (STATs) signaling pathway and transcription factors of CD4+ T cell subsets obtained from preeclamptic and normotensive (NT) pregnant women. Peripheral blood mononuclear cells (PBMCs) from 18 preeclamptic and 18 NT pregnant women were cultured with and without SB to analyze the expression of STATs and transcription factors by flow cytometry, and cytokines were measured in the culture supernatant by ELISA. The results showed that treating cells with SB decreased STAT1/ STAT4/T-bet and STAT3/RORγt, which characteristic of Th1 and Th17 inflammatory profiles, as well as increased STAT6/GATA-3 and STAT5/FoxP3 of anti-inflammatory and regulatory profiles, respectively. In addition, PBMCs from preeclamptic women treated with SB released lower concentrations of inflammatory cytokines and higher levels of IL-10 and TGF-β. Therefore, SB plays an immunomodulatory role on CD4+ T cell subsets in PE, leading to the downregulation of inflammatory profiles and upregulation of anti-inflammatory and regulatory profiles. More studies are necessary to better understand the modulation of CD4+ T cell subsets by the JAK/STAT and NF-κB pathways in this gestational pathology.Department of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESP, Sao PauloDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESP, Sao PauloDepartment of Health Science Oeste Paulista University-UNOESTE, Sao PauloDepartment of Gynecology and Obstetrics Botucatu Medical School Sao Paulo State University-UNESP, Sao PauloDepartment of Chemistry and Biological Sciences Institute of Biosciences Sao Paulo State University-UNESP, Sao PauloUniversidade Estadual Paulista (UNESP)Oeste Paulista University-UNOESTERibeiro, Vanessa Rocha [UNESP]Romao-Veiga, Mariana [UNESP]Nunes, Priscila Rezeck [UNESP]Oliveira, Larissa Ragozo Cardoso de [UNESP]Romagnoli, Graziela GorettiPeracoli, Jose Carlos [UNESP]Peracoli, Maria Terezinha Serrao [UNESP]2023-03-01T20:41:20Z2023-03-01T20:41:20Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.intimp.2022.108807International Immunopharmacology, v. 109.1878-17051567-5769http://hdl.handle.net/11449/24097210.1016/j.intimp.2022.1088072-s2.0-85129825450Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Immunopharmacologyinfo:eu-repo/semantics/openAccess2023-03-01T20:41:20Zoai:repositorio.unesp.br:11449/240972Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:41:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
title |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
spellingShingle |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia Ribeiro, Vanessa Rocha [UNESP] Preeclampsia Silibinin STATs T cell subsets Transcription factors |
title_short |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
title_full |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
title_fullStr |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
title_full_unstemmed |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
title_sort |
Silibinin downregulates the expression of the Th1 and Th17 profiles by modulation of STATs and transcription factors in pregnant women with preeclampsia |
author |
Ribeiro, Vanessa Rocha [UNESP] |
author_facet |
Ribeiro, Vanessa Rocha [UNESP] Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] Oliveira, Larissa Ragozo Cardoso de [UNESP] Romagnoli, Graziela Goretti Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author_role |
author |
author2 |
Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] Oliveira, Larissa Ragozo Cardoso de [UNESP] Romagnoli, Graziela Goretti Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Oeste Paulista University-UNOESTE |
dc.contributor.author.fl_str_mv |
Ribeiro, Vanessa Rocha [UNESP] Romao-Veiga, Mariana [UNESP] Nunes, Priscila Rezeck [UNESP] Oliveira, Larissa Ragozo Cardoso de [UNESP] Romagnoli, Graziela Goretti Peracoli, Jose Carlos [UNESP] Peracoli, Maria Terezinha Serrao [UNESP] |
dc.subject.por.fl_str_mv |
Preeclampsia Silibinin STATs T cell subsets Transcription factors |
topic |
Preeclampsia Silibinin STATs T cell subsets Transcription factors |
description |
Preeclampsia (PE) is a multifactorial disease that is characterized by inflammation. Some of the factors responsible for this inflammation are the cells of the innate and adaptive immune systems and their interactions. The use of natural products, such as silibinin (SB), can contribute to the control of this inflammation and gestational success. The present study evaluated whether the flavonoid SB has an in vitro immunomodulatory effect on the signal transducers and transcription activators (STATs) signaling pathway and transcription factors of CD4+ T cell subsets obtained from preeclamptic and normotensive (NT) pregnant women. Peripheral blood mononuclear cells (PBMCs) from 18 preeclamptic and 18 NT pregnant women were cultured with and without SB to analyze the expression of STATs and transcription factors by flow cytometry, and cytokines were measured in the culture supernatant by ELISA. The results showed that treating cells with SB decreased STAT1/ STAT4/T-bet and STAT3/RORγt, which characteristic of Th1 and Th17 inflammatory profiles, as well as increased STAT6/GATA-3 and STAT5/FoxP3 of anti-inflammatory and regulatory profiles, respectively. In addition, PBMCs from preeclamptic women treated with SB released lower concentrations of inflammatory cytokines and higher levels of IL-10 and TGF-β. Therefore, SB plays an immunomodulatory role on CD4+ T cell subsets in PE, leading to the downregulation of inflammatory profiles and upregulation of anti-inflammatory and regulatory profiles. More studies are necessary to better understand the modulation of CD4+ T cell subsets by the JAK/STAT and NF-κB pathways in this gestational pathology. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T20:41:20Z 2023-03-01T20:41:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.intimp.2022.108807 International Immunopharmacology, v. 109. 1878-1705 1567-5769 http://hdl.handle.net/11449/240972 10.1016/j.intimp.2022.108807 2-s2.0-85129825450 |
url |
http://dx.doi.org/10.1016/j.intimp.2022.108807 http://hdl.handle.net/11449/240972 |
identifier_str_mv |
International Immunopharmacology, v. 109. 1878-1705 1567-5769 10.1016/j.intimp.2022.108807 2-s2.0-85129825450 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Immunopharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964388304617472 |