Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae)
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0214574 http://hdl.handle.net/11449/188923 |
Resumo: | Background Phlebotomine sand flies are vectors for several pathogens, with Leishmania being the most important. In Brazil, the main aetiological agent of American cutaneous leishmaniasis (ACL) is Leishmania (Viannia) braziliensis, and Nyssomyia neivai is one of its main vectors in São Paulo state and other areas of South America. Similar to other haematophagous insects, sand flies use volatile compounds called kairomones to locate their hosts for blood meals. A possible increase in the attractiveness of hosts infected with Leishmania infantum to their vectors has been demonstrated. In the present study, we aimed to investigate whether L. braziliensis-infected hosts present higher attractiveness to Ny. neivai and to identify differences in the volatile compounds released by infected and uninfected mice. Results Behavioural experiments in which sand fly females directly fed on infected or uninfected mice showed no significant differences in the attractiveness of the mice or the blood volume ingested. Y-tube olfactometer bioassays also revealed no significant differences in the attractiveness of these hosts to Ny. neivai. No differences were observed in the profiles of the volatile compounds released by the two groups of mice. However, PCA and cluster analysis were able to classify the 31 identified compounds into three clusters according to their abundances. This classification showed a possible role for individual variation in the absence of differences in volatile profiles and attractiveness between infected and uninfected mice. Conclusion In this first cross-sectional study with an aetiological agent of ACL, there were no statistically significant differences in the attractiveness of infected hosts to their vector. |
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Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae)Background Phlebotomine sand flies are vectors for several pathogens, with Leishmania being the most important. In Brazil, the main aetiological agent of American cutaneous leishmaniasis (ACL) is Leishmania (Viannia) braziliensis, and Nyssomyia neivai is one of its main vectors in São Paulo state and other areas of South America. Similar to other haematophagous insects, sand flies use volatile compounds called kairomones to locate their hosts for blood meals. A possible increase in the attractiveness of hosts infected with Leishmania infantum to their vectors has been demonstrated. In the present study, we aimed to investigate whether L. braziliensis-infected hosts present higher attractiveness to Ny. neivai and to identify differences in the volatile compounds released by infected and uninfected mice. Results Behavioural experiments in which sand fly females directly fed on infected or uninfected mice showed no significant differences in the attractiveness of the mice or the blood volume ingested. Y-tube olfactometer bioassays also revealed no significant differences in the attractiveness of these hosts to Ny. neivai. No differences were observed in the profiles of the volatile compounds released by the two groups of mice. However, PCA and cluster analysis were able to classify the 31 identified compounds into three clusters according to their abundances. This classification showed a possible role for individual variation in the absence of differences in volatile profiles and attractiveness between infected and uninfected mice. Conclusion In this first cross-sectional study with an aetiological agent of ACL, there were no statistically significant differences in the attractiveness of infected hosts to their vector.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)British Rhinological SocietyDepartamento de Ciências Biológicas Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Câmpus AraraquaraDepartamento de Biologia Animal Universidade Estadual de Campinas (UNICAMP)Instituto Federal de Educação Ciência e Tecnologia de São Paulo (IFSP) Campus MatãoDepartamento de Estatística Universidade Estadual de Campinas (UNICAMP)Departamento de Ciências Biológicas Universidade Estadual Paulista (UNESP) Faculdade de Ciências Farmacêuticas Câmpus AraraquaraFAPESP: 2012/23832-9British Rhinological Society: 2015/21100-9Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Ciência e Tecnologia de São Paulo (IFSP)da Rocha Silva, Flávia Benini [UNESP]Miguel, Danilo CicconeMachado, Vicente Estevam [UNESP]Oliveira, Wanderson Henrique Cruz [UNESP]Goulart, Thais MarchiTosta, Christiann DavisPinheiro, Hildete PriscoPinto, Mara Cristina [UNESP]2019-10-06T16:23:30Z2019-10-06T16:23:30Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0214574PLoS ONE, v. 14, n. 4, 2019.1932-6203http://hdl.handle.net/11449/18892310.1371/journal.pone.02145742-s2.0-850637382708221737491598415Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2021-10-23T20:18:10Zoai:repositorio.unesp.br:11449/188923Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:18:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
title |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
spellingShingle |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) da Rocha Silva, Flávia Benini [UNESP] |
title_short |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
title_full |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
title_fullStr |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
title_full_unstemmed |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
title_sort |
Influence of Leishmania (Viannia) braziliensis infection on the attractiveness of BALB/c mice to Nyssomyia neivai (Diptera: Psychodidae) |
author |
da Rocha Silva, Flávia Benini [UNESP] |
author_facet |
da Rocha Silva, Flávia Benini [UNESP] Miguel, Danilo Ciccone Machado, Vicente Estevam [UNESP] Oliveira, Wanderson Henrique Cruz [UNESP] Goulart, Thais Marchi Tosta, Christiann Davis Pinheiro, Hildete Prisco Pinto, Mara Cristina [UNESP] |
author_role |
author |
author2 |
Miguel, Danilo Ciccone Machado, Vicente Estevam [UNESP] Oliveira, Wanderson Henrique Cruz [UNESP] Goulart, Thais Marchi Tosta, Christiann Davis Pinheiro, Hildete Prisco Pinto, Mara Cristina [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Ciência e Tecnologia de São Paulo (IFSP) |
dc.contributor.author.fl_str_mv |
da Rocha Silva, Flávia Benini [UNESP] Miguel, Danilo Ciccone Machado, Vicente Estevam [UNESP] Oliveira, Wanderson Henrique Cruz [UNESP] Goulart, Thais Marchi Tosta, Christiann Davis Pinheiro, Hildete Prisco Pinto, Mara Cristina [UNESP] |
description |
Background Phlebotomine sand flies are vectors for several pathogens, with Leishmania being the most important. In Brazil, the main aetiological agent of American cutaneous leishmaniasis (ACL) is Leishmania (Viannia) braziliensis, and Nyssomyia neivai is one of its main vectors in São Paulo state and other areas of South America. Similar to other haematophagous insects, sand flies use volatile compounds called kairomones to locate their hosts for blood meals. A possible increase in the attractiveness of hosts infected with Leishmania infantum to their vectors has been demonstrated. In the present study, we aimed to investigate whether L. braziliensis-infected hosts present higher attractiveness to Ny. neivai and to identify differences in the volatile compounds released by infected and uninfected mice. Results Behavioural experiments in which sand fly females directly fed on infected or uninfected mice showed no significant differences in the attractiveness of the mice or the blood volume ingested. Y-tube olfactometer bioassays also revealed no significant differences in the attractiveness of these hosts to Ny. neivai. No differences were observed in the profiles of the volatile compounds released by the two groups of mice. However, PCA and cluster analysis were able to classify the 31 identified compounds into three clusters according to their abundances. This classification showed a possible role for individual variation in the absence of differences in volatile profiles and attractiveness between infected and uninfected mice. Conclusion In this first cross-sectional study with an aetiological agent of ACL, there were no statistically significant differences in the attractiveness of infected hosts to their vector. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:23:30Z 2019-10-06T16:23:30Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0214574 PLoS ONE, v. 14, n. 4, 2019. 1932-6203 http://hdl.handle.net/11449/188923 10.1371/journal.pone.0214574 2-s2.0-85063738270 8221737491598415 |
url |
http://dx.doi.org/10.1371/journal.pone.0214574 http://hdl.handle.net/11449/188923 |
identifier_str_mv |
PLoS ONE, v. 14, n. 4, 2019. 1932-6203 10.1371/journal.pone.0214574 2-s2.0-85063738270 8221737491598415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792962437512691712 |