Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1089/fpd.2017.2341 http://hdl.handle.net/11449/163857 |
Resumo: | Cross-contamination is one of the main factors related to foodborne outbreaks. This study aimed to analyze the cross-contamination process of Salmonella enterica serovar Enteritidis from poultry to cucumbers, on various cutting board surfaces (plastic, wood, and glass) before and after washing and in the presence and absence of biofilm. Thus, 10 strains of Salmonella Enteritidis were used to test cross-contamination from poultry to the cutting boards and from thereon to cucumbers. Moreover, these strains were evaluated as to their capacity to form biofilm on hydrophobic (wood and plastic) and hydrophilic materials (glass). We recovered the 10 isolates from all unwashed boards and from all cucumbers that had contacted them. After washing, the recovery ranged from 10% to 100%, depending on the board material. In the presence of biofilm, the recovery of salmonellae was 100%, even after washing. Biofilm formation occurred more on wood (60%) and plastic (40%) than glass (10%) boards, demonstrating that bacteria adhered more to a hydrophobic material. It was concluded that the cutting boards represent a critical point in cross-contamination, particularly in the presence of biofilm. Salmonella Enteritidis was able to form a biofilm on these three types of cutting boards but glass showed the least formation. |
id |
UNSP_9cb4f2af2c89c668138a7500c57247d4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/163857 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boardsbiofilmcross-contaminationcsgD and adrA genescutting boardsSalmonella EnteritidisCross-contamination is one of the main factors related to foodborne outbreaks. This study aimed to analyze the cross-contamination process of Salmonella enterica serovar Enteritidis from poultry to cucumbers, on various cutting board surfaces (plastic, wood, and glass) before and after washing and in the presence and absence of biofilm. Thus, 10 strains of Salmonella Enteritidis were used to test cross-contamination from poultry to the cutting boards and from thereon to cucumbers. Moreover, these strains were evaluated as to their capacity to form biofilm on hydrophobic (wood and plastic) and hydrophilic materials (glass). We recovered the 10 isolates from all unwashed boards and from all cucumbers that had contacted them. After washing, the recovery ranged from 10% to 100%, depending on the board material. In the presence of biofilm, the recovery of salmonellae was 100%, even after washing. Biofilm formation occurred more on wood (60%) and plastic (40%) than glass (10%) boards, demonstrating that bacteria adhered more to a hydrophobic material. It was concluded that the cutting boards represent a critical point in cross-contamination, particularly in the presence of biofilm. Salmonella Enteritidis was able to form a biofilm on these three types of cutting boards but glass showed the least formation.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Inst Biosci Botucatu, Dept Microbiol & Immunol, Sao Paulo, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Microbiol & Immunol, Sao Paulo, BrazilMary Ann Liebert, IncUniversidade Estadual Paulista (Unesp)Dantas, Stefani T. A. [UNESP]Rossi, Bruna F. [UNESP]Bonsaglia, Erika C. R. [UNESP]Castilho, Ivana G. [UNESP]Hernandes, Rodrigo T. [UNESP]Fernandes, Ary [UNESP]Rall, Vera L. M. [UNESP]2018-11-26T17:48:11Z2018-11-26T17:48:11Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article81-85application/pdfhttp://dx.doi.org/10.1089/fpd.2017.2341Foodborne Pathogens And Disease. New Rochelle: Mary Ann Liebert, Inc, v. 15, n. 2, p. 81-85, 2018.1535-3141http://hdl.handle.net/11449/16385710.1089/fpd.2017.2341WOS:000425130200003WOS000425130200003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFoodborne Pathogens And Disease1,063info:eu-repo/semantics/openAccess2024-01-18T06:33:05Zoai:repositorio.unesp.br:11449/163857Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-18T06:33:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
title |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
spellingShingle |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards Dantas, Stefani T. A. [UNESP] biofilm cross-contamination csgD and adrA genes cutting boards Salmonella Enteritidis |
title_short |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
title_full |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
title_fullStr |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
title_full_unstemmed |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
title_sort |
Cross-Contamination and Biofilm Formation by Salmonella enterica Serovar Enteritidis on Various Cutting Boards |
author |
Dantas, Stefani T. A. [UNESP] |
author_facet |
Dantas, Stefani T. A. [UNESP] Rossi, Bruna F. [UNESP] Bonsaglia, Erika C. R. [UNESP] Castilho, Ivana G. [UNESP] Hernandes, Rodrigo T. [UNESP] Fernandes, Ary [UNESP] Rall, Vera L. M. [UNESP] |
author_role |
author |
author2 |
Rossi, Bruna F. [UNESP] Bonsaglia, Erika C. R. [UNESP] Castilho, Ivana G. [UNESP] Hernandes, Rodrigo T. [UNESP] Fernandes, Ary [UNESP] Rall, Vera L. M. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dantas, Stefani T. A. [UNESP] Rossi, Bruna F. [UNESP] Bonsaglia, Erika C. R. [UNESP] Castilho, Ivana G. [UNESP] Hernandes, Rodrigo T. [UNESP] Fernandes, Ary [UNESP] Rall, Vera L. M. [UNESP] |
dc.subject.por.fl_str_mv |
biofilm cross-contamination csgD and adrA genes cutting boards Salmonella Enteritidis |
topic |
biofilm cross-contamination csgD and adrA genes cutting boards Salmonella Enteritidis |
description |
Cross-contamination is one of the main factors related to foodborne outbreaks. This study aimed to analyze the cross-contamination process of Salmonella enterica serovar Enteritidis from poultry to cucumbers, on various cutting board surfaces (plastic, wood, and glass) before and after washing and in the presence and absence of biofilm. Thus, 10 strains of Salmonella Enteritidis were used to test cross-contamination from poultry to the cutting boards and from thereon to cucumbers. Moreover, these strains were evaluated as to their capacity to form biofilm on hydrophobic (wood and plastic) and hydrophilic materials (glass). We recovered the 10 isolates from all unwashed boards and from all cucumbers that had contacted them. After washing, the recovery ranged from 10% to 100%, depending on the board material. In the presence of biofilm, the recovery of salmonellae was 100%, even after washing. Biofilm formation occurred more on wood (60%) and plastic (40%) than glass (10%) boards, demonstrating that bacteria adhered more to a hydrophobic material. It was concluded that the cutting boards represent a critical point in cross-contamination, particularly in the presence of biofilm. Salmonella Enteritidis was able to form a biofilm on these three types of cutting boards but glass showed the least formation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:48:11Z 2018-11-26T17:48:11Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1089/fpd.2017.2341 Foodborne Pathogens And Disease. New Rochelle: Mary Ann Liebert, Inc, v. 15, n. 2, p. 81-85, 2018. 1535-3141 http://hdl.handle.net/11449/163857 10.1089/fpd.2017.2341 WOS:000425130200003 WOS000425130200003.pdf |
url |
http://dx.doi.org/10.1089/fpd.2017.2341 http://hdl.handle.net/11449/163857 |
identifier_str_mv |
Foodborne Pathogens And Disease. New Rochelle: Mary Ann Liebert, Inc, v. 15, n. 2, p. 81-85, 2018. 1535-3141 10.1089/fpd.2017.2341 WOS:000425130200003 WOS000425130200003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Foodborne Pathogens And Disease 1,063 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
81-85 application/pdf |
dc.publisher.none.fl_str_mv |
Mary Ann Liebert, Inc |
publisher.none.fl_str_mv |
Mary Ann Liebert, Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965660404514816 |