Host regulation-related peptides from the endoparasitoid Cotesia flavipes (Hymenoptera: Braconidae): identification, immunossupressive functions and insecticidal potential against Diatraea saccharalis (Lepidoptera: Crambidae)

• Autor(a) principal: Pinto, Ciro Pedro Guidotti [UNESP]
• Data de Publicação: 2021
• Tipo de documento: Tese
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/205126
• topic: Biological control; Proteomics; Biotechnology; Transcriptomics; Parasitism physiology; Controle biológico; Proteômica; Biotecnologia; Transcritômica; Fisiologia do parasitismo

The sugarcane borer, Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae), is one of the most relevant pests in sugarcane fields and has been successfully managed in certain regions of Brazil with the use of the parasitoid Cotesia flavipes Cameron, 1891 (Hymenoptera: Braconidae). Endoparasitoids regulate the behavior and physiology of their hosts by secreting bioactive proteins synthesized by venom glands, symbiotic viruses, teratocytes or by the developing larva in the host's hemocoel. Venom is injected into the host to aid parasitoid successful establishment in the first days of parasitism. Teratocytes are extraembryonic cells present in the parasitoid egg that dissociate from the serosa after the parasitoid hatches and disperse in the host insect's hemocoel. Several venom- and teratocytes-derived peptides have been identified in different parasitoid wasps and many remain uncharacterized or unexplored. Investigations of the identity of host regulation-related molecules from C. flavipes may result in the identification of proteins with biotechnological potential. The purpose of this research was to apply modern proteotranscriptomic approaches for the identification host regulation-related proteins from C. flavipes, highlighting peptides from teratocytes and venom. A plenty of peptides produced by teratocytes and venom glands was identified. Following, some of these peptides were selected and evaluated in terms of immunosuppressive and insecticidal potentials against D. saccharalis larvae. Results point out biological and biotechnological potential of these peptides that shed light on the comprehension of the physiology of parasitism as well for the development of new insecticidal molecules.