The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-19425-9 http://hdl.handle.net/11449/249159 |
Resumo: | This study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations of R. officinalis glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used to evaluate its biocompatibility in G. mellonella model, in which the nystatin suspension (100; 50; 25; 12.5 e 6.25%) was used as a control group. The antifungal action of R. officinalis glycolic extract was evaluated on C. albicans for 72, 48 and 12 h at two different phases: (1) using the extract as therapeutic agent; and (2) using the extract as prophylactic agent. PBS was used as a negative control group. G. mellonella survival curves were plotted using the Kaplan–Meier method and statistical analysis was performed using the log-rank test (Mantel–Cox) and the significance level was set at (α ≤ 0.05). There was no significant difference among the groups in which all were biocompatible except of a significant death rate of 26.6% with nystatin 100%. In phase 1, it was found that after 7 days, there was no statistically significant difference among the prophylactic treatment groups. In phase 2, the groups of R. officinalis 6.25 mg/mL for 72 h and R. officinalis of 12.5 mg/mL for 24 h promoted the survival rate of the larvae in comparison with the control group with a significant difference (p = 0.017) and (p = 0.032) respectively. Therefore, R. officinalis extract is biocompatible in different concentrations and can be used as a prophylactic agent against fungal infection. |
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The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella modelThis study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations of R. officinalis glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used to evaluate its biocompatibility in G. mellonella model, in which the nystatin suspension (100; 50; 25; 12.5 e 6.25%) was used as a control group. The antifungal action of R. officinalis glycolic extract was evaluated on C. albicans for 72, 48 and 12 h at two different phases: (1) using the extract as therapeutic agent; and (2) using the extract as prophylactic agent. PBS was used as a negative control group. G. mellonella survival curves were plotted using the Kaplan–Meier method and statistical analysis was performed using the log-rank test (Mantel–Cox) and the significance level was set at (α ≤ 0.05). There was no significant difference among the groups in which all were biocompatible except of a significant death rate of 26.6% with nystatin 100%. In phase 1, it was found that after 7 days, there was no statistically significant difference among the prophylactic treatment groups. In phase 2, the groups of R. officinalis 6.25 mg/mL for 72 h and R. officinalis of 12.5 mg/mL for 24 h promoted the survival rate of the larvae in comparison with the control group with a significant difference (p = 0.017) and (p = 0.032) respectively. Therefore, R. officinalis extract is biocompatible in different concentrations and can be used as a prophylactic agent against fungal infection.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SPDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University (ICT-UNESP), Av. Eng. Francisco José Longo Avenue 777, SPDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SPDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University (ICT-UNESP), Av. Eng. Francisco José Longo Avenue 777, SPFAPESP: 15/25338-0Universidade Estadual Paulista (UNESP)Meccatti, Vanessa Marques [UNESP]Figueiredo-Godoi, Lívia Mara Alves [UNESP]Pereira, Thaís Cristine [UNESP]de Lima, Patrícia Michelle Nagai [UNESP]Abu Hasna, Amjad [UNESP]Senna, Lavinia Barbosa [UNESP]Marcucci, Maria Cristina [UNESP]Junqueira, Juliana Campos [UNESP]de Oliveira, Luciane Dias [UNESP]2023-07-29T14:04:05Z2023-07-29T14:04:05Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-19425-9Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/24915910.1038/s41598-022-19425-92-s2.0-85138189776Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2023-07-29T14:04:05Zoai:repositorio.unesp.br:11449/249159Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T14:04:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
title |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
spellingShingle |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model Meccatti, Vanessa Marques [UNESP] |
title_short |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
title_full |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
title_fullStr |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
title_full_unstemmed |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
title_sort |
The biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model |
author |
Meccatti, Vanessa Marques [UNESP] |
author_facet |
Meccatti, Vanessa Marques [UNESP] Figueiredo-Godoi, Lívia Mara Alves [UNESP] Pereira, Thaís Cristine [UNESP] de Lima, Patrícia Michelle Nagai [UNESP] Abu Hasna, Amjad [UNESP] Senna, Lavinia Barbosa [UNESP] Marcucci, Maria Cristina [UNESP] Junqueira, Juliana Campos [UNESP] de Oliveira, Luciane Dias [UNESP] |
author_role |
author |
author2 |
Figueiredo-Godoi, Lívia Mara Alves [UNESP] Pereira, Thaís Cristine [UNESP] de Lima, Patrícia Michelle Nagai [UNESP] Abu Hasna, Amjad [UNESP] Senna, Lavinia Barbosa [UNESP] Marcucci, Maria Cristina [UNESP] Junqueira, Juliana Campos [UNESP] de Oliveira, Luciane Dias [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Meccatti, Vanessa Marques [UNESP] Figueiredo-Godoi, Lívia Mara Alves [UNESP] Pereira, Thaís Cristine [UNESP] de Lima, Patrícia Michelle Nagai [UNESP] Abu Hasna, Amjad [UNESP] Senna, Lavinia Barbosa [UNESP] Marcucci, Maria Cristina [UNESP] Junqueira, Juliana Campos [UNESP] de Oliveira, Luciane Dias [UNESP] |
description |
This study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations of R. officinalis glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used to evaluate its biocompatibility in G. mellonella model, in which the nystatin suspension (100; 50; 25; 12.5 e 6.25%) was used as a control group. The antifungal action of R. officinalis glycolic extract was evaluated on C. albicans for 72, 48 and 12 h at two different phases: (1) using the extract as therapeutic agent; and (2) using the extract as prophylactic agent. PBS was used as a negative control group. G. mellonella survival curves were plotted using the Kaplan–Meier method and statistical analysis was performed using the log-rank test (Mantel–Cox) and the significance level was set at (α ≤ 0.05). There was no significant difference among the groups in which all were biocompatible except of a significant death rate of 26.6% with nystatin 100%. In phase 1, it was found that after 7 days, there was no statistically significant difference among the prophylactic treatment groups. In phase 2, the groups of R. officinalis 6.25 mg/mL for 72 h and R. officinalis of 12.5 mg/mL for 24 h promoted the survival rate of the larvae in comparison with the control group with a significant difference (p = 0.017) and (p = 0.032) respectively. Therefore, R. officinalis extract is biocompatible in different concentrations and can be used as a prophylactic agent against fungal infection. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T14:04:05Z 2023-07-29T14:04:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-19425-9 Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/249159 10.1038/s41598-022-19425-9 2-s2.0-85138189776 |
url |
http://dx.doi.org/10.1038/s41598-022-19425-9 http://hdl.handle.net/11449/249159 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-19425-9 2-s2.0-85138189776 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965626221985792 |