Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1806-9061-2017-0589 http://hdl.handle.net/11449/170942 |
Resumo: | Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. |
id |
UNSP_9f595d7dd55f21dddcf0abac4639c9df |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/170942 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, BrazilAmazôniaAnimalsPicobirnavirusReovirusRotavirusRT-PCRMigratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities.Laboratório de Zoonoses Faculdade de Medicina Veterinária Universidade Federal do Pará, Campus Castanhal, Rodovia BR-316 Km 62 s/n, SaudadeInstituto Evandro Chagas (IEC), BR-316 Km 7 s/n, Distrito de LevilândiaLaboratório Nacional de Agricultura (Lana-gro/PA) Ministério da Agricultura Pecuária e Abastecimento (MAPA), Av. Almirante Barroso1234, Distrito de MarcoUniversidade Federal Rural da Amazônia (UFRA), Av. Presidente Tancredo Neves 2501Universidade Estadual Paulista (UNESP), Distrito de Rubião Jr. S/NUniversidade Estadual Paulista (UNESP), Distrito de Rubião Jr. S/NUniversidade Federal do Pará (UFPA)Instituto Evandro Chagas (IEC)Pecuária e Abastecimento (MAPA)Universidade Federal Rural da Amazônia (UFRA)Universidade Estadual Paulista (Unesp)Guerreiro, A. N.Moraes, C. C.G.Marinho, A. N.R.Barros, B. C.V.Bezerra, D. A.M.Bandeira, R. S.Silva, R. R.Rocha, D. C.C.Meneses, A. M.C.Luz, M. A.Paz, G. S. [UNESP]Mascarenhas, Joana D’Arc Pereira2018-12-11T16:53:04Z2018-12-11T16:53:04Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article161-168application/pdfhttp://dx.doi.org/10.1590/1806-9061-2017-0589Revista Brasileira de Ciencia Avicola, v. 20, n. 1, p. 161-168, 2018.1516-635Xhttp://hdl.handle.net/11449/17094210.1590/1806-9061-2017-0589S1516-635X20180001001612-s2.0-85046073213S1516-635X2018000100161.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciencia Avicolainfo:eu-repo/semantics/openAccess2024-01-24T06:33:15Zoai:repositorio.unesp.br:11449/170942Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-24T06:33:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
spellingShingle |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil Guerreiro, A. N. Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR |
title_short |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_full |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_fullStr |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_full_unstemmed |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
title_sort |
Investigation of enteric viruses in the feces of neotropical migratory birds captured on the coast of the state of Pará, Brazil |
author |
Guerreiro, A. N. |
author_facet |
Guerreiro, A. N. Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. [UNESP] Mascarenhas, Joana D’Arc Pereira |
author_role |
author |
author2 |
Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. [UNESP] Mascarenhas, Joana D’Arc Pereira |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Pará (UFPA) Instituto Evandro Chagas (IEC) Pecuária e Abastecimento (MAPA) Universidade Federal Rural da Amazônia (UFRA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Guerreiro, A. N. Moraes, C. C.G. Marinho, A. N.R. Barros, B. C.V. Bezerra, D. A.M. Bandeira, R. S. Silva, R. R. Rocha, D. C.C. Meneses, A. M.C. Luz, M. A. Paz, G. S. [UNESP] Mascarenhas, Joana D’Arc Pereira |
dc.subject.por.fl_str_mv |
Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR |
topic |
Amazônia Animals Picobirnavirus Reovirus Rotavirus RT-PCR |
description |
Migratory birds can become long-distance vectors for a wide range of microorganisms and can cause human disease, being the Brazilian coast a gateway for northern migratory birds. These animals are considered natural reservoirs of different viruses that cause important diseases, being relevant research of viral pathogens in migratory birds to epidemiology surveillance. The objective of the study was to investigate the presence of avian rotavirus (AvRV), avian reovirus (ARV) and picobirnavirus (PBV) in Neotropical migratory birds captured on the coast of Brazil. A total of 23 individual fecal samples of the migratory birds species Calidris pusilla (20 birds), Numenius phaeopus (1 bird) and Charadrius semipalmatus (2 birds) were collected. Fecal suspensions were prepared from the collected samples for subsequent extraction of double-stranded RNA (dsRNA), which was subjected to polyacrylamide gel electrophoresis (PAGE) and reverse transcription polymerase chain reaction (RT-PCR). The electrophoretic profiles were not detected by PAGE, and the amplification for the studied viruses PBV, ARV and AvRV (specie D, gene VP6 and NSP4) were negative. Positivity for AvRVD, VP7 gene was of 4.35% (1/23) for the migratory bird Calidris pusilla. After sequencing and building the tree of phylogenetic relationships avian Rotavirus Group D identified in this study was phylogenetically related and grouped into one branch, together to previously reported AvRVD from Brazil in chicken flocks with 99.8% nucleotide and 100% amino acid similarities. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:53:04Z 2018-12-11T16:53:04Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1806-9061-2017-0589 Revista Brasileira de Ciencia Avicola, v. 20, n. 1, p. 161-168, 2018. 1516-635X http://hdl.handle.net/11449/170942 10.1590/1806-9061-2017-0589 S1516-635X2018000100161 2-s2.0-85046073213 S1516-635X2018000100161.pdf |
url |
http://dx.doi.org/10.1590/1806-9061-2017-0589 http://hdl.handle.net/11449/170942 |
identifier_str_mv |
Revista Brasileira de Ciencia Avicola, v. 20, n. 1, p. 161-168, 2018. 1516-635X 10.1590/1806-9061-2017-0589 S1516-635X2018000100161 2-s2.0-85046073213 S1516-635X2018000100161.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ciencia Avicola |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
161-168 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790384830545920 |