New insights into trypanosomatid U5 small nuclear ribonucleoproteins
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762011000200003 http://hdl.handle.net/11449/7457 |
Resumo: | Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certain small complexes of RNA and protein, which are named small nuclear ribonucleoproteins (U snRNPs), have an essential role in pre-mRNA processing, mainly during splicing. Even though they are well defined in mammals, snRNPs are still not well characterized in trypanosomatids. This study shows that a U5-15K protein is highly conserved among various trypanosomatid species. Tandem affinity pull-down assays revealed that this protein interacts with a novel U5-102K protein, which suggests the presence of a sub-complex that is potentially involved in the assembly of U4/U6-U5 tri-snRNPs. Functional analyses showed that U5-15K is essential for cell viability and is somehow involved with the trans and cis splicing machinery. Similar tandem affinity experiments with a trypanonosomatid U5-Cwc21 protein led to the purification of four U5 snRNP specific proteins and a Sm core, suggesting U5-Cwc-21 participation in the 35S U5 snRNP particle. of these proteins, U5-200K was molecularly characterized. U5-200K has conserved domains, such as the DEAD/DEAH box helicase and Sec63 domains and displays a strong interaction with U5 snRNA. |
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New insights into trypanosomatid U5 small nuclear ribonucleoproteinstrans splicingcis splicingU5 snRNPU5-Cwc-21PTP-TagTrypanosoma cruziTrypanosoma bruceiSeveral protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certain small complexes of RNA and protein, which are named small nuclear ribonucleoproteins (U snRNPs), have an essential role in pre-mRNA processing, mainly during splicing. Even though they are well defined in mammals, snRNPs are still not well characterized in trypanosomatids. This study shows that a U5-15K protein is highly conserved among various trypanosomatid species. Tandem affinity pull-down assays revealed that this protein interacts with a novel U5-102K protein, which suggests the presence of a sub-complex that is potentially involved in the assembly of U4/U6-U5 tri-snRNPs. Functional analyses showed that U5-15K is essential for cell viability and is somehow involved with the trans and cis splicing machinery. Similar tandem affinity experiments with a trypanonosomatid U5-Cwc21 protein led to the purification of four U5 snRNP specific proteins and a Sm core, suggesting U5-Cwc-21 participation in the 35S U5 snRNP particle. of these proteins, U5-200K was molecularly characterized. U5-200K has conserved domains, such as the DEAD/DEAH box helicase and Sec63 domains and displays a strong interaction with U5 snRNA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, Araraquara, SP, BrazilUniv São Paulo, Fac Med Ribeirao Preto, Ctr Quim Prot, Ribeirao Preto, SP, BrazilUniv São Paulo, Fac Med Ribeirao Preto, Ctr Reg Hemoterapia, Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Dept Ciencias Biol, Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, Araraquara, SP, BrazilFAPESP: 06/05766-8FAPESP: 07/07476-0FAPESP: 08/56226-9Fundacão Oswaldo CruzUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)da Silva, Marco Tulio A. [UNESP]Ambrosio, Daniela L. [UNESP]Trevelin, Caroline C. [UNESP]Watanabe, Tatiana F. [UNESP]Laure, Helen J.Greene, Lewis J.Rosa, Jose C.Valentini, Sandro Roberto [UNESP]Cicarelli, Regina Maria Barretto [UNESP]2014-05-20T13:24:14Z2014-05-20T13:24:14Z2011-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article130-138application/pdfhttp://dx.doi.org/10.1590/S0074-02762011000200003Memorias do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 106, n. 2, p. 130-138, 2011.0074-0276http://hdl.handle.net/11449/7457S0074-02762011000200003WOS:000289961100003S0074-02762011000200003-en.pdf5333250355049814Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-25T06:07:02Zoai:repositorio.unesp.br:11449/7457Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-25T06:07:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
title |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
spellingShingle |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins da Silva, Marco Tulio A. [UNESP] trans splicing cis splicing U5 snRNP U5-Cwc-21 PTP-Tag Trypanosoma cruzi Trypanosoma brucei |
title_short |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
title_full |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
title_fullStr |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
title_full_unstemmed |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
title_sort |
New insights into trypanosomatid U5 small nuclear ribonucleoproteins |
author |
da Silva, Marco Tulio A. [UNESP] |
author_facet |
da Silva, Marco Tulio A. [UNESP] Ambrosio, Daniela L. [UNESP] Trevelin, Caroline C. [UNESP] Watanabe, Tatiana F. [UNESP] Laure, Helen J. Greene, Lewis J. Rosa, Jose C. Valentini, Sandro Roberto [UNESP] Cicarelli, Regina Maria Barretto [UNESP] |
author_role |
author |
author2 |
Ambrosio, Daniela L. [UNESP] Trevelin, Caroline C. [UNESP] Watanabe, Tatiana F. [UNESP] Laure, Helen J. Greene, Lewis J. Rosa, Jose C. Valentini, Sandro Roberto [UNESP] Cicarelli, Regina Maria Barretto [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
da Silva, Marco Tulio A. [UNESP] Ambrosio, Daniela L. [UNESP] Trevelin, Caroline C. [UNESP] Watanabe, Tatiana F. [UNESP] Laure, Helen J. Greene, Lewis J. Rosa, Jose C. Valentini, Sandro Roberto [UNESP] Cicarelli, Regina Maria Barretto [UNESP] |
dc.subject.por.fl_str_mv |
trans splicing cis splicing U5 snRNP U5-Cwc-21 PTP-Tag Trypanosoma cruzi Trypanosoma brucei |
topic |
trans splicing cis splicing U5 snRNP U5-Cwc-21 PTP-Tag Trypanosoma cruzi Trypanosoma brucei |
description |
Several protozoan parasites exist in the Trypanosomatidae family, including various agents of human diseases. Multiple lines of evidence suggest that important differences are present between the translational and mRNA processing (trans splicing) systems of trypanosomatids and other eukaryotes. In this context, certain small complexes of RNA and protein, which are named small nuclear ribonucleoproteins (U snRNPs), have an essential role in pre-mRNA processing, mainly during splicing. Even though they are well defined in mammals, snRNPs are still not well characterized in trypanosomatids. This study shows that a U5-15K protein is highly conserved among various trypanosomatid species. Tandem affinity pull-down assays revealed that this protein interacts with a novel U5-102K protein, which suggests the presence of a sub-complex that is potentially involved in the assembly of U4/U6-U5 tri-snRNPs. Functional analyses showed that U5-15K is essential for cell viability and is somehow involved with the trans and cis splicing machinery. Similar tandem affinity experiments with a trypanonosomatid U5-Cwc21 protein led to the purification of four U5 snRNP specific proteins and a Sm core, suggesting U5-Cwc-21 participation in the 35S U5 snRNP particle. of these proteins, U5-200K was molecularly characterized. U5-200K has conserved domains, such as the DEAD/DEAH box helicase and Sec63 domains and displays a strong interaction with U5 snRNA. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 2014-05-20T13:24:14Z 2014-05-20T13:24:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762011000200003 Memorias do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 106, n. 2, p. 130-138, 2011. 0074-0276 http://hdl.handle.net/11449/7457 S0074-02762011000200003 WOS:000289961100003 S0074-02762011000200003-en.pdf 5333250355049814 |
url |
http://dx.doi.org/10.1590/S0074-02762011000200003 http://hdl.handle.net/11449/7457 |
identifier_str_mv |
Memorias do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 106, n. 2, p. 130-138, 2011. 0074-0276 S0074-02762011000200003 WOS:000289961100003 S0074-02762011000200003-en.pdf 5333250355049814 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
130-138 application/pdf |
dc.publisher.none.fl_str_mv |
Fundacão Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundacão Oswaldo Cruz |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964690081644544 |