A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxicon.2012.12.009 http://hdl.handle.net/11449/74946 |
Resumo: | The invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods. © 2013 Elsevier Ltd. |
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A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis)AllergenChromatographyHymenopteraMyrmecologyToxinalkaloidant venomhexanehydrocarbonaqueous solutionevaporationextractionlyophilisatemass spectrometrynonhumanphase separationprecipitationpriority journalprotein analysisseparation techniqueSolenopsisstorage temperaturetwo dimensional gel electrophoresisAnimalsAnt VenomsAntsChemical FractionationElectrophoresis, Gel, Two-DimensionalThe invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods. © 2013 Elsevier Ltd.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Laboratório de Entomologia Médica e Molecular Instituto de Biofísica Carlos Chagas Filho Federal University of Rio de Janeiro (IBCCF/UFRJ), Rio de JaneiroInstituto de Biociências UNESP - Universidade Estadual Paulista, Campus de Rio Claro Departamento de Biologia - Centro de Estudos de Insetos Sociais, SPCenter of Studies of Venoms and Venomous Animals (CEVAP) University of São Paulo State (UNESP), Botucatu, SPInstituto de Biociências UNESP - Universidade Estadual Paulista, Campus de Rio Claro Departamento de Biologia - Centro de Estudos de Insetos Sociais, SPCenter of Studies of Venoms and Venomous Animals (CEVAP) University of São Paulo State (UNESP), Botucatu, SPUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Estadual Paulista (Unesp)Gonçalves Paterson Fox, EduardoRuss Solis, Daniel [UNESP]Delazari dos Santos, Lucilene [UNESP]Aparecido dos Santos Pinto, Jose Roberto [UNESP]Ribeiro da Silva Menegasso, Anally [UNESP]Cardoso Maciel Costa Silva, RafaelPalma, Mario Sergio [UNESP]Correa Bueno, Odair [UNESP]de Alcântara Machado, Ednildo2014-05-27T11:28:45Z2014-05-27T11:28:45Z2013-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article5-8application/pdfhttp://dx.doi.org/10.1016/j.toxicon.2012.12.009Toxicon, v. 65, p. 5-8.0041-01011879-3150http://hdl.handle.net/11449/7494610.1016/j.toxicon.2012.12.009WOS:0003171722000022-s2.0-848733070722-s2.0-84873307072.pdf29018886245065351050709055776428Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicon2.3520,692info:eu-repo/semantics/openAccess2023-10-04T06:06:32Zoai:repositorio.unesp.br:11449/74946Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-04T06:06:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
title |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
spellingShingle |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) Gonçalves Paterson Fox, Eduardo Allergen Chromatography Hymenoptera Myrmecology Toxin alkaloid ant venom hexane hydrocarbon aqueous solution evaporation extraction lyophilisate mass spectrometry nonhuman phase separation precipitation priority journal protein analysis separation technique Solenopsis storage temperature two dimensional gel electrophoresis Animals Ant Venoms Ants Chemical Fractionation Electrophoresis, Gel, Two-Dimensional |
title_short |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
title_full |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
title_fullStr |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
title_full_unstemmed |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
title_sort |
A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis) |
author |
Gonçalves Paterson Fox, Eduardo |
author_facet |
Gonçalves Paterson Fox, Eduardo Russ Solis, Daniel [UNESP] Delazari dos Santos, Lucilene [UNESP] Aparecido dos Santos Pinto, Jose Roberto [UNESP] Ribeiro da Silva Menegasso, Anally [UNESP] Cardoso Maciel Costa Silva, Rafael Palma, Mario Sergio [UNESP] Correa Bueno, Odair [UNESP] de Alcântara Machado, Ednildo |
author_role |
author |
author2 |
Russ Solis, Daniel [UNESP] Delazari dos Santos, Lucilene [UNESP] Aparecido dos Santos Pinto, Jose Roberto [UNESP] Ribeiro da Silva Menegasso, Anally [UNESP] Cardoso Maciel Costa Silva, Rafael Palma, Mario Sergio [UNESP] Correa Bueno, Odair [UNESP] de Alcântara Machado, Ednildo |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gonçalves Paterson Fox, Eduardo Russ Solis, Daniel [UNESP] Delazari dos Santos, Lucilene [UNESP] Aparecido dos Santos Pinto, Jose Roberto [UNESP] Ribeiro da Silva Menegasso, Anally [UNESP] Cardoso Maciel Costa Silva, Rafael Palma, Mario Sergio [UNESP] Correa Bueno, Odair [UNESP] de Alcântara Machado, Ednildo |
dc.subject.por.fl_str_mv |
Allergen Chromatography Hymenoptera Myrmecology Toxin alkaloid ant venom hexane hydrocarbon aqueous solution evaporation extraction lyophilisate mass spectrometry nonhuman phase separation precipitation priority journal protein analysis separation technique Solenopsis storage temperature two dimensional gel electrophoresis Animals Ant Venoms Ants Chemical Fractionation Electrophoresis, Gel, Two-Dimensional |
topic |
Allergen Chromatography Hymenoptera Myrmecology Toxin alkaloid ant venom hexane hydrocarbon aqueous solution evaporation extraction lyophilisate mass spectrometry nonhuman phase separation precipitation priority journal protein analysis separation technique Solenopsis storage temperature two dimensional gel electrophoresis Animals Ant Venoms Ants Chemical Fractionation Electrophoresis, Gel, Two-Dimensional |
description |
The invasive fire ant Solenopsis invicta is medically important because its venom is highly potent. However, almost nothing is known about fire ant venom proteins because obtaining even milligram-amounts of these proteins has been prohibitively challenging. We present a simple and fast method of obtaining whole venom compounds from large quantities of fire ants. For this, we separate the ants are from the nest soil, immerse them in dual-phase mixture of apolar organic solvent and water, and evaporate each solvent phase in separate. The remaining extract from the aqueous phase is largely made up of ant venom proteins. We confirmed this by using 2D gel electrophoresis while also demonstrating that our new approach yields the same proteins obtained by other authors using less efficient traditional methods. © 2013 Elsevier Ltd. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-01 2014-05-27T11:28:45Z 2014-05-27T11:28:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxicon.2012.12.009 Toxicon, v. 65, p. 5-8. 0041-0101 1879-3150 http://hdl.handle.net/11449/74946 10.1016/j.toxicon.2012.12.009 WOS:000317172200002 2-s2.0-84873307072 2-s2.0-84873307072.pdf 2901888624506535 1050709055776428 |
url |
http://dx.doi.org/10.1016/j.toxicon.2012.12.009 http://hdl.handle.net/11449/74946 |
identifier_str_mv |
Toxicon, v. 65, p. 5-8. 0041-0101 1879-3150 10.1016/j.toxicon.2012.12.009 WOS:000317172200002 2-s2.0-84873307072 2-s2.0-84873307072.pdf 2901888624506535 1050709055776428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicon 2.352 0,692 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
5-8 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789289516367872 |