Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2016.1144053 http://hdl.handle.net/11449/172605 |
Resumo: | The aim of this study was to evaluate the effect of the QS molecule farnesol on single and mixed species biofilms formed by Candida albicans and Streptococcus mutans. The anti-biofilm effect of farnesol was assessed through total biomass quantification, counting of colony forming units (CFUs) and evaluation of metabolic activity. Biofilms were also analyzed by scanning electron microscopy (SEM). It was observed that farnesol reduced the formation of single and mixed biofilms, with significant reductions of 37% to 90% and 64% to 96%, respectively, for total biomass and metabolic activity. Regarding cell viability, farnesol treatment promoted significant log reductions in the number of CFUs, ie 1.3–4.2 log10 and 0.67–5.32 log10, respectively, for single and mixed species biofilms. SEM images confirmed these results, showing decreases in the number of cells in all biofilms. In conclusion, these findings highlight the role of farnesol as an alternative agent with the potential to reduce the formation of pathogenic biofilms. |
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Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluationBiofilmsCandida albicansquorum sensingStreptococcus mutansThe aim of this study was to evaluate the effect of the QS molecule farnesol on single and mixed species biofilms formed by Candida albicans and Streptococcus mutans. The anti-biofilm effect of farnesol was assessed through total biomass quantification, counting of colony forming units (CFUs) and evaluation of metabolic activity. Biofilms were also analyzed by scanning electron microscopy (SEM). It was observed that farnesol reduced the formation of single and mixed biofilms, with significant reductions of 37% to 90% and 64% to 96%, respectively, for total biomass and metabolic activity. Regarding cell viability, farnesol treatment promoted significant log reductions in the number of CFUs, ie 1.3–4.2 log10 and 0.67–5.32 log10, respectively, for single and mixed species biofilms. SEM images confirmed these results, showing decreases in the number of cells in all biofilms. In conclusion, these findings highlight the role of farnesol as an alternative agent with the potential to reduce the formation of pathogenic biofilms.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Pediatric Dentistry and Public Health Aracatuba Dental School UNESPDepartment of Dental Materials and Prosthodontics Aracatuba Dental School UNESPDepartment of Pediatric Dentistry and Public Health Aracatuba Dental School UNESPDepartment of Dental Materials and Prosthodontics Aracatuba Dental School UNESPFAPESP: 2013/23592-0Universidade Estadual Paulista (Unesp)Fernandes, Renan Aparecido [UNESP]Monteiro, Douglas Roberto [UNESP]Arias, Laís Salomão [UNESP]Fernandes, Gabriela Lopes [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Barbosa, Debora Barros [UNESP]2018-12-11T17:01:24Z2018-12-11T17:01:24Z2016-03-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article329-338application/pdfhttp://dx.doi.org/10.1080/08927014.2016.1144053Biofouling, v. 32, n. 3, p. 329-338, 2016.1029-24540892-7014http://hdl.handle.net/11449/17260510.1080/08927014.2016.11440532-s2.0-849593609952-s2.0-84959360995.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofouling0,8350,835info:eu-repo/semantics/openAccess2024-01-16T06:30:56Zoai:repositorio.unesp.br:11449/172605Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-16T06:30:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
title |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
spellingShingle |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation Fernandes, Renan Aparecido [UNESP] Biofilms Candida albicans quorum sensing Streptococcus mutans |
title_short |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
title_full |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
title_fullStr |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
title_full_unstemmed |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
title_sort |
Biofilm formation by Candida albicans and Streptococcus mutans in the presence of farnesol: a quantitative evaluation |
author |
Fernandes, Renan Aparecido [UNESP] |
author_facet |
Fernandes, Renan Aparecido [UNESP] Monteiro, Douglas Roberto [UNESP] Arias, Laís Salomão [UNESP] Fernandes, Gabriela Lopes [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Barbosa, Debora Barros [UNESP] |
author_role |
author |
author2 |
Monteiro, Douglas Roberto [UNESP] Arias, Laís Salomão [UNESP] Fernandes, Gabriela Lopes [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Barbosa, Debora Barros [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Fernandes, Renan Aparecido [UNESP] Monteiro, Douglas Roberto [UNESP] Arias, Laís Salomão [UNESP] Fernandes, Gabriela Lopes [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Barbosa, Debora Barros [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Candida albicans quorum sensing Streptococcus mutans |
topic |
Biofilms Candida albicans quorum sensing Streptococcus mutans |
description |
The aim of this study was to evaluate the effect of the QS molecule farnesol on single and mixed species biofilms formed by Candida albicans and Streptococcus mutans. The anti-biofilm effect of farnesol was assessed through total biomass quantification, counting of colony forming units (CFUs) and evaluation of metabolic activity. Biofilms were also analyzed by scanning electron microscopy (SEM). It was observed that farnesol reduced the formation of single and mixed biofilms, with significant reductions of 37% to 90% and 64% to 96%, respectively, for total biomass and metabolic activity. Regarding cell viability, farnesol treatment promoted significant log reductions in the number of CFUs, ie 1.3–4.2 log10 and 0.67–5.32 log10, respectively, for single and mixed species biofilms. SEM images confirmed these results, showing decreases in the number of cells in all biofilms. In conclusion, these findings highlight the role of farnesol as an alternative agent with the potential to reduce the formation of pathogenic biofilms. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-15 2018-12-11T17:01:24Z 2018-12-11T17:01:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2016.1144053 Biofouling, v. 32, n. 3, p. 329-338, 2016. 1029-2454 0892-7014 http://hdl.handle.net/11449/172605 10.1080/08927014.2016.1144053 2-s2.0-84959360995 2-s2.0-84959360995.pdf |
url |
http://dx.doi.org/10.1080/08927014.2016.1144053 http://hdl.handle.net/11449/172605 |
identifier_str_mv |
Biofouling, v. 32, n. 3, p. 329-338, 2016. 1029-2454 0892-7014 10.1080/08927014.2016.1144053 2-s2.0-84959360995 2-s2.0-84959360995.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling 0,835 0,835 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
329-338 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790313923739648 |