Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jdent.2020.103453 http://hdl.handle.net/11449/200945 |
Resumo: | Objectives: This study evaluated the antimicrobial and antibiofilm effects of a colloidal nanocarrier for chlorhexidine (CHX) on Candida glabrata and Enterococcus faecalis, as well as tested its cytotoxic effect on murine fibroblasts. Methods: Iron oxide nanoparticles (IONPs) were coated with chitosan (CS) and loaded with CHX at 31.2, 78 and 156 μg/mL. Antimicrobial effects were assessed by determining the minimum inhibitory concentration (MIC), using the broth microdilution method, and fractional inhibitory concentration index (FICI). Preformed biofilms (48 h) were treated with different concentrations of the nanocarrier (24 h) and quantified by colony-forming units (CFUs), total biomass and metabolic activity. For cytotoxicity, the viability of L929 cells was evaluated by MTT assay after 24 and 48 h of exposure to the nanocarrier. Data were submitted to ANOVA and Fisher LSD or Tukey post-hoc tests (α = 0.05). Results: MIC and FICI results showed an indifferent interaction among the components of the nanocarrier for all strains evaluated. CHX alone and nanocarrier containing 156 μg/mL CHX did not differ from each other in reducing the number of CFUs. However, the nanocarrier containing 156 μg/mL CHX promoted the highest reductions in total biofilm biomass and metabolism, surpassing the effect of CHX alone. After 24 and 48 h of exposure, the nanocarrier reduced CHX toxicity to the L929 cell at low concentrations. Conclusion: These findings suggest that the CHX nanocarrier has potential to be used in the control of oral diseases associated with C. glabrata and E. faecalis. Clinical relevance: CHX has improved the antibiofilm effect and reduced the cytotoxicity (at low concentrations) when conjugated to CS-coated IONPs. This new colloidal formulation has potential as an alternative antimicrobial agent to pure CHX for the control of biofilm-related oral diseases, such as oral candidiasis and endodontic infections. |
id |
UNSP_a4a6103dc1ffe8948a0638b59395e54e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/200945 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidineBiofilmsCandidaChlorhexidineCytotoxicityEnterococcus faecalisIron oxide nanoparticlesObjectives: This study evaluated the antimicrobial and antibiofilm effects of a colloidal nanocarrier for chlorhexidine (CHX) on Candida glabrata and Enterococcus faecalis, as well as tested its cytotoxic effect on murine fibroblasts. Methods: Iron oxide nanoparticles (IONPs) were coated with chitosan (CS) and loaded with CHX at 31.2, 78 and 156 μg/mL. Antimicrobial effects were assessed by determining the minimum inhibitory concentration (MIC), using the broth microdilution method, and fractional inhibitory concentration index (FICI). Preformed biofilms (48 h) were treated with different concentrations of the nanocarrier (24 h) and quantified by colony-forming units (CFUs), total biomass and metabolic activity. For cytotoxicity, the viability of L929 cells was evaluated by MTT assay after 24 and 48 h of exposure to the nanocarrier. Data were submitted to ANOVA and Fisher LSD or Tukey post-hoc tests (α = 0.05). Results: MIC and FICI results showed an indifferent interaction among the components of the nanocarrier for all strains evaluated. CHX alone and nanocarrier containing 156 μg/mL CHX did not differ from each other in reducing the number of CFUs. However, the nanocarrier containing 156 μg/mL CHX promoted the highest reductions in total biofilm biomass and metabolism, surpassing the effect of CHX alone. After 24 and 48 h of exposure, the nanocarrier reduced CHX toxicity to the L929 cell at low concentrations. Conclusion: These findings suggest that the CHX nanocarrier has potential to be used in the control of oral diseases associated with C. glabrata and E. faecalis. Clinical relevance: CHX has improved the antibiofilm effect and reduced the cytotoxicity (at low concentrations) when conjugated to CS-coated IONPs. This new colloidal formulation has potential as an alternative antimicrobial agent to pure CHX for the control of biofilm-related oral diseases, such as oral candidiasis and endodontic infections.São Paulo State University (Unesp) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, AraçatubaSchool of Dentistry Presidente Prudente University of Western São Paulo (UNOESTE), Presidente PrudenteSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Basic Sciences, AraçatubaGraduate Program in Dentistry (GPD - Master's Degree) University of Western São Paulo (UNOESTE), Presidente PrudenteSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, AraçatubaSão Paulo State University (Unesp) School of Dentistry Araçatuba Department of Basic Sciences, AraçatubaUniversidade Estadual Paulista (Unesp)University of Western São Paulo (UNOESTE)Araujo, Heitor Ceolin [UNESP]da Silva, Ana Carolina GomesPaião, Luana IsabelMagario, Mychelle Keiko WatanabeFrasnelli, Sabrina Cruz Tfaile [UNESP]Oliveira, Sandra Helena Penha de [UNESP]Pessan, Juliano Pelim [UNESP]Monteiro, Douglas Roberto2020-12-12T02:20:13Z2020-12-12T02:20:13Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jdent.2020.103453Journal of Dentistry, v. 101.0300-5712http://hdl.handle.net/11449/20094510.1016/j.jdent.2020.1034532-s2.0-85089804922Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dentistryinfo:eu-repo/semantics/openAccess2023-02-06T14:41:06Zoai:repositorio.unesp.br:11449/200945Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-02-06T14:41:06Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
title |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
spellingShingle |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine Araujo, Heitor Ceolin [UNESP] Biofilms Candida Chlorhexidine Cytotoxicity Enterococcus faecalis Iron oxide nanoparticles |
title_short |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
title_full |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
title_fullStr |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
title_full_unstemmed |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
title_sort |
Antimicrobial, antibiofilm and cytotoxic effects of a colloidal nanocarrier composed by chitosan-coated iron oxide nanoparticles loaded with chlorhexidine |
author |
Araujo, Heitor Ceolin [UNESP] |
author_facet |
Araujo, Heitor Ceolin [UNESP] da Silva, Ana Carolina Gomes Paião, Luana Isabel Magario, Mychelle Keiko Watanabe Frasnelli, Sabrina Cruz Tfaile [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Pessan, Juliano Pelim [UNESP] Monteiro, Douglas Roberto |
author_role |
author |
author2 |
da Silva, Ana Carolina Gomes Paião, Luana Isabel Magario, Mychelle Keiko Watanabe Frasnelli, Sabrina Cruz Tfaile [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Pessan, Juliano Pelim [UNESP] Monteiro, Douglas Roberto |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Western São Paulo (UNOESTE) |
dc.contributor.author.fl_str_mv |
Araujo, Heitor Ceolin [UNESP] da Silva, Ana Carolina Gomes Paião, Luana Isabel Magario, Mychelle Keiko Watanabe Frasnelli, Sabrina Cruz Tfaile [UNESP] Oliveira, Sandra Helena Penha de [UNESP] Pessan, Juliano Pelim [UNESP] Monteiro, Douglas Roberto |
dc.subject.por.fl_str_mv |
Biofilms Candida Chlorhexidine Cytotoxicity Enterococcus faecalis Iron oxide nanoparticles |
topic |
Biofilms Candida Chlorhexidine Cytotoxicity Enterococcus faecalis Iron oxide nanoparticles |
description |
Objectives: This study evaluated the antimicrobial and antibiofilm effects of a colloidal nanocarrier for chlorhexidine (CHX) on Candida glabrata and Enterococcus faecalis, as well as tested its cytotoxic effect on murine fibroblasts. Methods: Iron oxide nanoparticles (IONPs) were coated with chitosan (CS) and loaded with CHX at 31.2, 78 and 156 μg/mL. Antimicrobial effects were assessed by determining the minimum inhibitory concentration (MIC), using the broth microdilution method, and fractional inhibitory concentration index (FICI). Preformed biofilms (48 h) were treated with different concentrations of the nanocarrier (24 h) and quantified by colony-forming units (CFUs), total biomass and metabolic activity. For cytotoxicity, the viability of L929 cells was evaluated by MTT assay after 24 and 48 h of exposure to the nanocarrier. Data were submitted to ANOVA and Fisher LSD or Tukey post-hoc tests (α = 0.05). Results: MIC and FICI results showed an indifferent interaction among the components of the nanocarrier for all strains evaluated. CHX alone and nanocarrier containing 156 μg/mL CHX did not differ from each other in reducing the number of CFUs. However, the nanocarrier containing 156 μg/mL CHX promoted the highest reductions in total biofilm biomass and metabolism, surpassing the effect of CHX alone. After 24 and 48 h of exposure, the nanocarrier reduced CHX toxicity to the L929 cell at low concentrations. Conclusion: These findings suggest that the CHX nanocarrier has potential to be used in the control of oral diseases associated with C. glabrata and E. faecalis. Clinical relevance: CHX has improved the antibiofilm effect and reduced the cytotoxicity (at low concentrations) when conjugated to CS-coated IONPs. This new colloidal formulation has potential as an alternative antimicrobial agent to pure CHX for the control of biofilm-related oral diseases, such as oral candidiasis and endodontic infections. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:20:13Z 2020-12-12T02:20:13Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jdent.2020.103453 Journal of Dentistry, v. 101. 0300-5712 http://hdl.handle.net/11449/200945 10.1016/j.jdent.2020.103453 2-s2.0-85089804922 |
url |
http://dx.doi.org/10.1016/j.jdent.2020.103453 http://hdl.handle.net/11449/200945 |
identifier_str_mv |
Journal of Dentistry, v. 101. 0300-5712 10.1016/j.jdent.2020.103453 2-s2.0-85089804922 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790345269870592 |