Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2334-12-278 http://hdl.handle.net/11449/10767 |
Resumo: | Background: Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a specific lymphocyte transformation assay to Cryptococcus neoformans in order to identify immunodeficiency associated to neurocryptococcosis (NCC) as primary cause of the mycosis.Methods: Healthy volunteers, poultry growers, and HIV-seronegative patients with neurocryptococcosis were tested for cellular immune response. Cryptococcal meningitis was diagnosed by India ink staining of cerebrospinal fluid and cryptococcal antigen test (Immunomycol-Inc, SP, Brazil). Isolated peripheral blood mononuclear cells were stimulated with C. neoformans antigen, C. albicans antigen, and pokeweed mitogen. The amount of H-3-thymidine incorporated was assessed, and the results were expressed as stimulation index (SI) and log SI, sensitivity, specificity, and cut-off value (receiver operating characteristics curve). We applied unpaired Student t tests to compare data and considered significant differences for p<0.05.Results: The lymphotoxin alpha showed a low capacity with all the stimuli for classifying patients as responders and non-responders. Lymphotoxin alpha stimulated by heated-killed antigen from patients with neurocryptococcosis was not affected by TCD4+ cell count, and the intensity of response did not correlate with the clinical evolution of neurocryptococcosis.Conclusion: Response to lymphocyte transformation assay should be analyzed based on a normal range and using more than one stimulator. The use of a cut-off value to classify patients with neurocryptococcosis is inadequate. Statistical analysis should be based on the log transformation of SI. A more purified antigen for evaluating specific response to C. neoformans is needed. |
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Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with NeurocryptococcosisLymphocytesAntigensBiasesStatisticalCryptococcusMethodsCryptococcus neoformansBackground: Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a specific lymphocyte transformation assay to Cryptococcus neoformans in order to identify immunodeficiency associated to neurocryptococcosis (NCC) as primary cause of the mycosis.Methods: Healthy volunteers, poultry growers, and HIV-seronegative patients with neurocryptococcosis were tested for cellular immune response. Cryptococcal meningitis was diagnosed by India ink staining of cerebrospinal fluid and cryptococcal antigen test (Immunomycol-Inc, SP, Brazil). Isolated peripheral blood mononuclear cells were stimulated with C. neoformans antigen, C. albicans antigen, and pokeweed mitogen. The amount of H-3-thymidine incorporated was assessed, and the results were expressed as stimulation index (SI) and log SI, sensitivity, specificity, and cut-off value (receiver operating characteristics curve). We applied unpaired Student t tests to compare data and considered significant differences for p<0.05.Results: The lymphotoxin alpha showed a low capacity with all the stimuli for classifying patients as responders and non-responders. Lymphotoxin alpha stimulated by heated-killed antigen from patients with neurocryptococcosis was not affected by TCD4+ cell count, and the intensity of response did not correlate with the clinical evolution of neurocryptococcosis.Conclusion: Response to lymphocyte transformation assay should be analyzed based on a normal range and using more than one stimulator. The use of a cut-off value to classify patients with neurocryptococcosis is inadequate. Statistical analysis should be based on the log transformation of SI. A more purified antigen for evaluating specific response to C. neoformans is needed.Faculdade de Medicina, Universidade de São PauloUniversidade Estadual PaulistaUniv São Paulo, Fac Med, Dept Dermatol, Lab Dermatol & Immunodeficiencias, BR-01246903 São Paulo, BrazilFac Med ABC, Dept Morfol & Fisiol, BR-09060650 Santo Andre, SP, BrazilUniv Fed São Paulo, Dept Ciencias Biol, Diadema, SP, BrazilUniv Estadual Paulista, UNESP, Dept Fonoaudiol, Fac Filosofia & Ciencias, BR-17525900 Marilia, SP, BrazilFac Med ABC, Dept Patol, BR-09060650 Santo Andre, SP, BrazilUniv Estadual Paulista, UNESP, Dept Fonoaudiol, Fac Filosofia & Ciencias, BR-17525900 Marilia, SP, BrazilBiomed Central Ltd.Universidade de São Paulo (USP)Fac Med ABCUniversidade Federal de São Paulo (UNIFESP)Universidade Estadual Paulista (Unesp)Rocha, Katya C.Pinhal, CinthiaCavalcanti, SoniaVidal, Monica S. M.Toscano, MatheusMoraes-Vasconcelos, DewtonDuarte, Alberto J. S.Fonseca, Fernando L. A.Abreu, Luiz Carlos de [UNESP]Valenti, Vitor Engrácia [UNESP]Grumach, Anete S. G.2014-05-20T13:31:36Z2014-05-20T13:31:36Z2012-10-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1471-2334-12-278Bmc Infectious Diseases. London: Biomed Central Ltd., v. 12, p. 8, 2012.1471-2334http://hdl.handle.net/11449/1076710.1186/1471-2334-12-278WOS:000314094100001WOS000314094100001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Infectious Diseases2.6201,576info:eu-repo/semantics/openAccess2023-12-23T06:14:42Zoai:repositorio.unesp.br:11449/10767Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-23T06:14:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
title |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
spellingShingle |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis Rocha, Katya C. Lymphocytes Antigens Biases Statistical Cryptococcus Methods Cryptococcus neoformans |
title_short |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
title_full |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
title_fullStr |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
title_full_unstemmed |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
title_sort |
Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis |
author |
Rocha, Katya C. |
author_facet |
Rocha, Katya C. Pinhal, Cinthia Cavalcanti, Sonia Vidal, Monica S. M. Toscano, Matheus Moraes-Vasconcelos, Dewton Duarte, Alberto J. S. Fonseca, Fernando L. A. Abreu, Luiz Carlos de [UNESP] Valenti, Vitor Engrácia [UNESP] Grumach, Anete S. G. |
author_role |
author |
author2 |
Pinhal, Cinthia Cavalcanti, Sonia Vidal, Monica S. M. Toscano, Matheus Moraes-Vasconcelos, Dewton Duarte, Alberto J. S. Fonseca, Fernando L. A. Abreu, Luiz Carlos de [UNESP] Valenti, Vitor Engrácia [UNESP] Grumach, Anete S. G. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Fac Med ABC Universidade Federal de São Paulo (UNIFESP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rocha, Katya C. Pinhal, Cinthia Cavalcanti, Sonia Vidal, Monica S. M. Toscano, Matheus Moraes-Vasconcelos, Dewton Duarte, Alberto J. S. Fonseca, Fernando L. A. Abreu, Luiz Carlos de [UNESP] Valenti, Vitor Engrácia [UNESP] Grumach, Anete S. G. |
dc.subject.por.fl_str_mv |
Lymphocytes Antigens Biases Statistical Cryptococcus Methods Cryptococcus neoformans |
topic |
Lymphocytes Antigens Biases Statistical Cryptococcus Methods Cryptococcus neoformans |
description |
Background: Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a specific lymphocyte transformation assay to Cryptococcus neoformans in order to identify immunodeficiency associated to neurocryptococcosis (NCC) as primary cause of the mycosis.Methods: Healthy volunteers, poultry growers, and HIV-seronegative patients with neurocryptococcosis were tested for cellular immune response. Cryptococcal meningitis was diagnosed by India ink staining of cerebrospinal fluid and cryptococcal antigen test (Immunomycol-Inc, SP, Brazil). Isolated peripheral blood mononuclear cells were stimulated with C. neoformans antigen, C. albicans antigen, and pokeweed mitogen. The amount of H-3-thymidine incorporated was assessed, and the results were expressed as stimulation index (SI) and log SI, sensitivity, specificity, and cut-off value (receiver operating characteristics curve). We applied unpaired Student t tests to compare data and considered significant differences for p<0.05.Results: The lymphotoxin alpha showed a low capacity with all the stimuli for classifying patients as responders and non-responders. Lymphotoxin alpha stimulated by heated-killed antigen from patients with neurocryptococcosis was not affected by TCD4+ cell count, and the intensity of response did not correlate with the clinical evolution of neurocryptococcosis.Conclusion: Response to lymphocyte transformation assay should be analyzed based on a normal range and using more than one stimulator. The use of a cut-off value to classify patients with neurocryptococcosis is inadequate. Statistical analysis should be based on the log transformation of SI. A more purified antigen for evaluating specific response to C. neoformans is needed. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-30 2014-05-20T13:31:36Z 2014-05-20T13:31:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2334-12-278 Bmc Infectious Diseases. London: Biomed Central Ltd., v. 12, p. 8, 2012. 1471-2334 http://hdl.handle.net/11449/10767 10.1186/1471-2334-12-278 WOS:000314094100001 WOS000314094100001.pdf |
url |
http://dx.doi.org/10.1186/1471-2334-12-278 http://hdl.handle.net/11449/10767 |
identifier_str_mv |
Bmc Infectious Diseases. London: Biomed Central Ltd., v. 12, p. 8, 2012. 1471-2334 10.1186/1471-2334-12-278 WOS:000314094100001 WOS000314094100001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Infectious Diseases 2.620 1,576 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792962149764562944 |