Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/biolinnean/bly210 http://hdl.handle.net/11449/187544 |
Resumo: | Chromosomal regions enriched in repetitive DNA sequences have a predisposition to instability, resulting in a propensity to undergo structural rearrangements. Hence, the rRNA multigene family, a type of tandem repeat sequences, is a valuable cytogenetic marker for studying genomic organization and chromosomal differentiation. To investigate whether the evolutionary dynamics of heterochromatin and rDNA clusters played a role in the genomic reorganization and chromosomal evolution of a social wasp genus, we used the C-banding technique and fluorescence in situ hybridization (FISH) to conduct chromosomal mapping of the major rDNA class in three closely related species of warrior wasps: Synoeca cyanea, S. ilheensis and S. surinama. In this study, the S. surinama karyotype is reported for the first time. It contains 2n = 34 regular-sized chromosomes and approximately 30 microchromosomes, thus representing an unprecedented number of this chromosomal type for the order Hymenoptera. We discuss the putative evolutionary mechanism for the origin of microchromosomes in S. surinama. For the three species, C-positive heterochromatic blocks were predominantly detected in the pericentromeric region of all chromosomes. However, our FISH results revealed striking variability in the number and chromosomal position of rDNA clusters among the three species studied, as well as intraspecific variations in S. cyanea and S. ilheensis, which suggests intense evolutionary dynamics of this repetitive DNA family and highlights rapid chromosomal evolution in these insects. |
id |
UNSP_a58f030e63bec8569f17878bc2aaba5d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/187544 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in HymenopteraChromosomal evolutionMolecular cytogeneticsrDNAsocial waspsVespidaeChromosomal regions enriched in repetitive DNA sequences have a predisposition to instability, resulting in a propensity to undergo structural rearrangements. Hence, the rRNA multigene family, a type of tandem repeat sequences, is a valuable cytogenetic marker for studying genomic organization and chromosomal differentiation. To investigate whether the evolutionary dynamics of heterochromatin and rDNA clusters played a role in the genomic reorganization and chromosomal evolution of a social wasp genus, we used the C-banding technique and fluorescence in situ hybridization (FISH) to conduct chromosomal mapping of the major rDNA class in three closely related species of warrior wasps: Synoeca cyanea, S. ilheensis and S. surinama. In this study, the S. surinama karyotype is reported for the first time. It contains 2n = 34 regular-sized chromosomes and approximately 30 microchromosomes, thus representing an unprecedented number of this chromosomal type for the order Hymenoptera. We discuss the putative evolutionary mechanism for the origin of microchromosomes in S. surinama. For the three species, C-positive heterochromatic blocks were predominantly detected in the pericentromeric region of all chromosomes. However, our FISH results revealed striking variability in the number and chromosomal position of rDNA clusters among the three species studied, as well as intraspecific variations in S. cyanea and S. ilheensis, which suggests intense evolutionary dynamics of this repetitive DNA family and highlights rapid chromosomal evolution in these insects.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado da BahiaConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)National Museum of Natural HistoryRussian Academy of SciencesDepartamento de Ciências Biológicas Universidade Estadual de Santa Cruz, Rodovia Jorge Amado, Km 16Departamento de Biologia Faculdade de Filosofia Ciências e Letras Universidade de São Paulo (FFCLRP/USP), Av. Bandeirantes, 3900Departamento de Biologia Universidade Estadual Paulista (UNESP) Instituto de Biociências, Av. 24A, 1515Departamento de Biologia Universidade Estadual Paulista (UNESP) Instituto de Biociências, Av. 24A, 1515FAPESP: 2011/09477-9FAPESP: 2015/02432-0FAPESP: 2016/21098-7Fundação de Amparo à Pesquisa do Estado da Bahia: BOL0494/2011CNPq: CNPq-310178/2015-0Universidade Estadual de Santa CruzUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Menezes, Rodolpho S.T.Gazoni, Thiago [UNESP]Costa, Marco A.2019-10-06T15:39:35Z2019-10-06T15:39:35Z2019-03-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article925-935http://dx.doi.org/10.1093/biolinnean/bly210Biological Journal of the Linnean Society, v. 126, n. 4, p. 925-935, 2019.1095-83120024-4066http://hdl.handle.net/11449/18754410.1093/biolinnean/bly2102-s2.0-85064166672Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Journal of the Linnean Societyinfo:eu-repo/semantics/openAccess2021-10-23T20:19:26Zoai:repositorio.unesp.br:11449/187544Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:19:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
title |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
spellingShingle |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera Menezes, Rodolpho S.T. Chromosomal evolution Molecular cytogenetics rDNA social wasps Vespidae |
title_short |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
title_full |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
title_fullStr |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
title_full_unstemmed |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
title_sort |
Cytogenetics of warrior wasps (Vespidae: Synoeca) reveals intense evolutionary dynamics of ribosomal DNA clusters and an unprecedented number of microchromosomes in Hymenoptera |
author |
Menezes, Rodolpho S.T. |
author_facet |
Menezes, Rodolpho S.T. Gazoni, Thiago [UNESP] Costa, Marco A. |
author_role |
author |
author2 |
Gazoni, Thiago [UNESP] Costa, Marco A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Santa Cruz Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Menezes, Rodolpho S.T. Gazoni, Thiago [UNESP] Costa, Marco A. |
dc.subject.por.fl_str_mv |
Chromosomal evolution Molecular cytogenetics rDNA social wasps Vespidae |
topic |
Chromosomal evolution Molecular cytogenetics rDNA social wasps Vespidae |
description |
Chromosomal regions enriched in repetitive DNA sequences have a predisposition to instability, resulting in a propensity to undergo structural rearrangements. Hence, the rRNA multigene family, a type of tandem repeat sequences, is a valuable cytogenetic marker for studying genomic organization and chromosomal differentiation. To investigate whether the evolutionary dynamics of heterochromatin and rDNA clusters played a role in the genomic reorganization and chromosomal evolution of a social wasp genus, we used the C-banding technique and fluorescence in situ hybridization (FISH) to conduct chromosomal mapping of the major rDNA class in three closely related species of warrior wasps: Synoeca cyanea, S. ilheensis and S. surinama. In this study, the S. surinama karyotype is reported for the first time. It contains 2n = 34 regular-sized chromosomes and approximately 30 microchromosomes, thus representing an unprecedented number of this chromosomal type for the order Hymenoptera. We discuss the putative evolutionary mechanism for the origin of microchromosomes in S. surinama. For the three species, C-positive heterochromatic blocks were predominantly detected in the pericentromeric region of all chromosomes. However, our FISH results revealed striking variability in the number and chromosomal position of rDNA clusters among the three species studied, as well as intraspecific variations in S. cyanea and S. ilheensis, which suggests intense evolutionary dynamics of this repetitive DNA family and highlights rapid chromosomal evolution in these insects. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:39:35Z 2019-10-06T15:39:35Z 2019-03-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/biolinnean/bly210 Biological Journal of the Linnean Society, v. 126, n. 4, p. 925-935, 2019. 1095-8312 0024-4066 http://hdl.handle.net/11449/187544 10.1093/biolinnean/bly210 2-s2.0-85064166672 |
url |
http://dx.doi.org/10.1093/biolinnean/bly210 http://hdl.handle.net/11449/187544 |
identifier_str_mv |
Biological Journal of the Linnean Society, v. 126, n. 4, p. 925-935, 2019. 1095-8312 0024-4066 10.1093/biolinnean/bly210 2-s2.0-85064166672 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Journal of the Linnean Society |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
925-935 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790421466742784 |