TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1039/c7ra08263k http://hdl.handle.net/11449/175401 |
Resumo: | The lack of osseointegration and implant-related infections are two major complications leading to failure of dental and orthopedic implants. Therefore, the development of effective titanium (Ti) implant surfaces able to display enhanced osteogenic activity and antimicrobial properties is required. In particular, titanium dioxide (TiO2) nanotubes (NTs) have demonstrated promising features to modulate biological responses, as they may be easily tailored to achieve multiple functions. This work aims to study the ability of bio-functionalized TiO2 NTs to induce osseointegration, and concomitantly, to avoid infection. TiO2 NTs were bio-functionalized with calcium (Ca), phosphorous (P) and zinc (Zn), by reverse polarization anodization. Morphological and topographical features of NTs were observed through scanning electron microscopy (SEM), while surface chemistry was investigated by X-ray photoelectron spectroscopy (XPS). Biocompatibility studies were conducted with MG-63 and human mesenchymal stem cells (hMSCs) through MTT assay. Furthermore, cell morphology and cytoskeleton organization were observed by SEM and laser scanning confocal microscopy (LSCM). The osteoblastic differentiation capacity of hMSCs was studied by real-time PCR, as well as their angiogenesis ability by measuring the total release of vascular endothelial growth factor (VEGF). Finally, viability of Staphylococcus aureus (S. aureus) was assessed by live/dead bacterial viability assay. Results show that bio-functionalized TiO2 nanotubular surfaces are biocompatible and modulated cell morphology. In particular, NTs enriched with Ca, P, and Zn, induced to significantly up-regulated levels of bone morphogenetic protein 2 (BMP-2) and osteopontin (OPN) genes of hMSCs, when compared to conventional NTs. TiO2 nanotubular surfaces induced hMSCs to release a higher amount of VEGF, and significantly reduced the bacterial viability, both when compared to adequate Ti controls. In conclusion, the superimposition of TiO2 nanotubular-textured surfaces and their enrichment with Ca, P, and Zn, is a very promising approach for the development of novel bio-selective implant surfaces able to improve osseointegration and avoid infection. |
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TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implantsThe lack of osseointegration and implant-related infections are two major complications leading to failure of dental and orthopedic implants. Therefore, the development of effective titanium (Ti) implant surfaces able to display enhanced osteogenic activity and antimicrobial properties is required. In particular, titanium dioxide (TiO2) nanotubes (NTs) have demonstrated promising features to modulate biological responses, as they may be easily tailored to achieve multiple functions. This work aims to study the ability of bio-functionalized TiO2 NTs to induce osseointegration, and concomitantly, to avoid infection. TiO2 NTs were bio-functionalized with calcium (Ca), phosphorous (P) and zinc (Zn), by reverse polarization anodization. Morphological and topographical features of NTs were observed through scanning electron microscopy (SEM), while surface chemistry was investigated by X-ray photoelectron spectroscopy (XPS). Biocompatibility studies were conducted with MG-63 and human mesenchymal stem cells (hMSCs) through MTT assay. Furthermore, cell morphology and cytoskeleton organization were observed by SEM and laser scanning confocal microscopy (LSCM). The osteoblastic differentiation capacity of hMSCs was studied by real-time PCR, as well as their angiogenesis ability by measuring the total release of vascular endothelial growth factor (VEGF). Finally, viability of Staphylococcus aureus (S. aureus) was assessed by live/dead bacterial viability assay. Results show that bio-functionalized TiO2 nanotubular surfaces are biocompatible and modulated cell morphology. In particular, NTs enriched with Ca, P, and Zn, induced to significantly up-regulated levels of bone morphogenetic protein 2 (BMP-2) and osteopontin (OPN) genes of hMSCs, when compared to conventional NTs. TiO2 nanotubular surfaces induced hMSCs to release a higher amount of VEGF, and significantly reduced the bacterial viability, both when compared to adequate Ti controls. In conclusion, the superimposition of TiO2 nanotubular-textured surfaces and their enrichment with Ca, P, and Zn, is a very promising approach for the development of novel bio-selective implant surfaces able to improve osseointegration and avoid infection.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CMEMS-Center of MicroElectroMechanical Systems Department of Mechanical Engineering University of Minho AzurémIBTN/BR-Brazilian Branch Institute of Biomaterials Tribocorrosion and Nanomedicine Faculty of Sciences UNESP-Universidade Estadual PaulistaDir. of Life Sciences Applied Metrology National Institute of Metrology Quality and TechnologyPostgraduate Program in Translational Biomedicine University of Grande Rio-UNIGRANRIOPostgraduate Program in Biotechnology National Institute of Metrology Quality and TechnologyBrazilian Center for Research in PhysicsMaterials Metrology Division National Institute of Metrology Quality and TechnologyCEB-Centre of Biological Engineering University of Minho Campus de GualtarDepartment of Materials Engineering KU LeuvenDepartment of Bioengineering University of Illinois at ChicagoIBTN/US-American Branch Institute of Biomaterials Tribocorrosion and Nanomedicine University of Illinois at ChicagoCenter of Regenerative Medicine Faculty of Medicine of Petrópolis-FASESchool of Dentistry Fluminense Federal UniversityFaculdade de Ciências Departamento de Física Universidade Estadual Paulista-UNESPIBTN/BR-Brazilian Branch Institute of Biomaterials Tribocorrosion and Nanomedicine Faculty of Sciences UNESP-Universidade Estadual PaulistaFaculdade de Ciências Departamento de Física Universidade Estadual Paulista-UNESPCAPES: 99999.008666/2014-08University of Minho AzurémUniversidade Estadual Paulista (Unesp)National Institute of Metrology Quality and TechnologyUniversity of Grande Rio-UNIGRANRIOQuality and TechnologyBrazilian Center for Research in PhysicsUniversity of MinhoKU LeuvenUniversity of Illinois at ChicagoFaculty of Medicine of Petrópolis-FASEFluminense Federal UniversityAlves, S. A. [UNESP]Ribeiro, A. R. [UNESP]Gemini-Piperni, S. [UNESP]Silva, R. C.Saraiva, A. M.Leite, P. E.Perez, G.Oliveira, S. M.Araujo, J. R.Archanjo, B. S.Rodrigues, M. E.Henriques, M.Celis, J. P.Shokuhfar, T.Borojevic, R. [UNESP]Granjeiro, J. M. [UNESP]Rocha, L. A. [UNESP]2018-12-11T17:15:39Z2018-12-11T17:15:39Z2017-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article49720-49738application/pdfhttp://dx.doi.org/10.1039/c7ra08263kRSC Advances, v. 7, n. 78, p. 49720-49738, 2017.2046-2069http://hdl.handle.net/11449/17540110.1039/c7ra08263k2-s2.0-850324501932-s2.0-85032450193.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRSC Advances0,863info:eu-repo/semantics/openAccess2024-04-25T17:39:52Zoai:repositorio.unesp.br:11449/175401Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-25T17:39:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
title |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
spellingShingle |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants Alves, S. A. [UNESP] |
title_short |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
title_full |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
title_fullStr |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
title_full_unstemmed |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
title_sort |
TiO2 nanotubes enriched with calcium, phosphorous and zinc: Promising bio-selective functional surfaces for osseointegrated titanium implants |
author |
Alves, S. A. [UNESP] |
author_facet |
Alves, S. A. [UNESP] Ribeiro, A. R. [UNESP] Gemini-Piperni, S. [UNESP] Silva, R. C. Saraiva, A. M. Leite, P. E. Perez, G. Oliveira, S. M. Araujo, J. R. Archanjo, B. S. Rodrigues, M. E. Henriques, M. Celis, J. P. Shokuhfar, T. Borojevic, R. [UNESP] Granjeiro, J. M. [UNESP] Rocha, L. A. [UNESP] |
author_role |
author |
author2 |
Ribeiro, A. R. [UNESP] Gemini-Piperni, S. [UNESP] Silva, R. C. Saraiva, A. M. Leite, P. E. Perez, G. Oliveira, S. M. Araujo, J. R. Archanjo, B. S. Rodrigues, M. E. Henriques, M. Celis, J. P. Shokuhfar, T. Borojevic, R. [UNESP] Granjeiro, J. M. [UNESP] Rocha, L. A. [UNESP] |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Minho Azurém Universidade Estadual Paulista (Unesp) National Institute of Metrology Quality and Technology University of Grande Rio-UNIGRANRIO Quality and Technology Brazilian Center for Research in Physics University of Minho KU Leuven University of Illinois at Chicago Faculty of Medicine of Petrópolis-FASE Fluminense Federal University |
dc.contributor.author.fl_str_mv |
Alves, S. A. [UNESP] Ribeiro, A. R. [UNESP] Gemini-Piperni, S. [UNESP] Silva, R. C. Saraiva, A. M. Leite, P. E. Perez, G. Oliveira, S. M. Araujo, J. R. Archanjo, B. S. Rodrigues, M. E. Henriques, M. Celis, J. P. Shokuhfar, T. Borojevic, R. [UNESP] Granjeiro, J. M. [UNESP] Rocha, L. A. [UNESP] |
description |
The lack of osseointegration and implant-related infections are two major complications leading to failure of dental and orthopedic implants. Therefore, the development of effective titanium (Ti) implant surfaces able to display enhanced osteogenic activity and antimicrobial properties is required. In particular, titanium dioxide (TiO2) nanotubes (NTs) have demonstrated promising features to modulate biological responses, as they may be easily tailored to achieve multiple functions. This work aims to study the ability of bio-functionalized TiO2 NTs to induce osseointegration, and concomitantly, to avoid infection. TiO2 NTs were bio-functionalized with calcium (Ca), phosphorous (P) and zinc (Zn), by reverse polarization anodization. Morphological and topographical features of NTs were observed through scanning electron microscopy (SEM), while surface chemistry was investigated by X-ray photoelectron spectroscopy (XPS). Biocompatibility studies were conducted with MG-63 and human mesenchymal stem cells (hMSCs) through MTT assay. Furthermore, cell morphology and cytoskeleton organization were observed by SEM and laser scanning confocal microscopy (LSCM). The osteoblastic differentiation capacity of hMSCs was studied by real-time PCR, as well as their angiogenesis ability by measuring the total release of vascular endothelial growth factor (VEGF). Finally, viability of Staphylococcus aureus (S. aureus) was assessed by live/dead bacterial viability assay. Results show that bio-functionalized TiO2 nanotubular surfaces are biocompatible and modulated cell morphology. In particular, NTs enriched with Ca, P, and Zn, induced to significantly up-regulated levels of bone morphogenetic protein 2 (BMP-2) and osteopontin (OPN) genes of hMSCs, when compared to conventional NTs. TiO2 nanotubular surfaces induced hMSCs to release a higher amount of VEGF, and significantly reduced the bacterial viability, both when compared to adequate Ti controls. In conclusion, the superimposition of TiO2 nanotubular-textured surfaces and their enrichment with Ca, P, and Zn, is a very promising approach for the development of novel bio-selective implant surfaces able to improve osseointegration and avoid infection. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 2018-12-11T17:15:39Z 2018-12-11T17:15:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1039/c7ra08263k RSC Advances, v. 7, n. 78, p. 49720-49738, 2017. 2046-2069 http://hdl.handle.net/11449/175401 10.1039/c7ra08263k 2-s2.0-85032450193 2-s2.0-85032450193.pdf |
url |
http://dx.doi.org/10.1039/c7ra08263k http://hdl.handle.net/11449/175401 |
identifier_str_mv |
RSC Advances, v. 7, n. 78, p. 49720-49738, 2017. 2046-2069 10.1039/c7ra08263k 2-s2.0-85032450193 2-s2.0-85032450193.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RSC Advances 0,863 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
49720-49738 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965017916833792 |