Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2022.10.041 http://hdl.handle.net/11449/247930 |
Resumo: | Oocyte in vitro maturation (IVM) is still a major challenge in human and animal assisted reproduction. Gradual instead of abrupt activation of the ovulatory cascade during IVM has been proposed to enhance nuclear-cytoplasmic synchrony and cumulus-oocyte communication, thus favoring oocyte developmental competence. Herein, we assessed the effects of neuregulin 1 (NRG1), an EGF-like factor that modulates EGFR signaling, on oocyte nuclear maturation dynamics, cumulus expansion and expression of mRNAs regulating these processes during IVM, as well as on post-IVF embryo development following AREG-stimulated IVM in cattle. In experiment 1, cumulus-oocyte complexes (COCs) were subjected to IVM with graded doses of NRG1 (1, 10 or 100 ng/mL) for 6, 9, 12, 20, and 24 h, after which oocyte nuclear status and cumulus mRNA expression were assessed. At 6 h of IVM, NRG1 at 1 ng/mL significantly decreased the percentage of GVBD (germinal vesicle breakdown) oocytes without altering later meiotic dynamics or the percentage of oocytes achieving meiosis II. In experiment 2, adding NRG1 (1 ng/mL) to the IVM medium did not affect cumulus expansion but increased the percentage of expanded and hatched blastocysts, and blastocyst total cell number following IVF/IVC. NRG1 decreased EGFR mRNA abundance while increasing NPR2 and PTX3 mRNA levels at 9 h, and TNFAIP6 mRNA abundance at 20 h of IVM. This is the first study that reports the modulatory effect of NGR1 during oocyte maturation in a mono-ovulatory species and demonstrates that this action may be applied during IVM to improve post-IVF embryo development. |
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Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo developmentAREGBovine embryo productionEGF-LikeGene expressionOocyte nuclear maturationOocyte in vitro maturation (IVM) is still a major challenge in human and animal assisted reproduction. Gradual instead of abrupt activation of the ovulatory cascade during IVM has been proposed to enhance nuclear-cytoplasmic synchrony and cumulus-oocyte communication, thus favoring oocyte developmental competence. Herein, we assessed the effects of neuregulin 1 (NRG1), an EGF-like factor that modulates EGFR signaling, on oocyte nuclear maturation dynamics, cumulus expansion and expression of mRNAs regulating these processes during IVM, as well as on post-IVF embryo development following AREG-stimulated IVM in cattle. In experiment 1, cumulus-oocyte complexes (COCs) were subjected to IVM with graded doses of NRG1 (1, 10 or 100 ng/mL) for 6, 9, 12, 20, and 24 h, after which oocyte nuclear status and cumulus mRNA expression were assessed. At 6 h of IVM, NRG1 at 1 ng/mL significantly decreased the percentage of GVBD (germinal vesicle breakdown) oocytes without altering later meiotic dynamics or the percentage of oocytes achieving meiosis II. In experiment 2, adding NRG1 (1 ng/mL) to the IVM medium did not affect cumulus expansion but increased the percentage of expanded and hatched blastocysts, and blastocyst total cell number following IVF/IVC. NRG1 decreased EGFR mRNA abundance while increasing NPR2 and PTX3 mRNA levels at 9 h, and TNFAIP6 mRNA abundance at 20 h of IVM. This is the first study that reports the modulatory effect of NGR1 during oocyte maturation in a mono-ovulatory species and demonstrates that this action may be applied during IVM to improve post-IVF embryo development.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Structural and Functional Biology Institute of Biosciences Sao Paulo State University, Sao PauloDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Sao Paulo State University, Sao PauloBiogenesi Reproductive Medicine CentreReproductive and Developmental Biology Laboratory Department of Veterinary Medicine and Animal Science University of MilanDepartment of Structural and Functional Biology Institute of Biosciences Sao Paulo State University, Sao PauloDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science Sao Paulo State University, Sao PauloCAPES: 0001FAPESP: 2019/14588–6Universidade Estadual Paulista (UNESP)Reproductive Medicine CentreUniversity of MilanDellaqua, Thaisy Tino [UNESP]Vígaro, Renan Aparecido [UNESP]Janini, Ludimila Cardoso Zoccal [UNESP]Dal Canto, MariabeatriceRenzini, Mario MigniniLodde, ValentinaLuciano, Alberto MariaBuratini, Jose [UNESP]2023-07-29T13:29:50Z2023-07-29T13:29:50Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article209-216http://dx.doi.org/10.1016/j.theriogenology.2022.10.041Theriogenology, v. 195, p. 209-216.0093-691Xhttp://hdl.handle.net/11449/24793010.1016/j.theriogenology.2022.10.0412-s2.0-85142402416Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2023-07-29T13:29:50Zoai:repositorio.unesp.br:11449/247930Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:29:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
title |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
spellingShingle |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development Dellaqua, Thaisy Tino [UNESP] AREG Bovine embryo production EGF-Like Gene expression Oocyte nuclear maturation |
title_short |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
title_full |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
title_fullStr |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
title_full_unstemmed |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
title_sort |
Neuregulin 1 (NRG1) modulates oocyte nuclear maturation during IVM and improves post-IVF embryo development |
author |
Dellaqua, Thaisy Tino [UNESP] |
author_facet |
Dellaqua, Thaisy Tino [UNESP] Vígaro, Renan Aparecido [UNESP] Janini, Ludimila Cardoso Zoccal [UNESP] Dal Canto, Mariabeatrice Renzini, Mario Mignini Lodde, Valentina Luciano, Alberto Maria Buratini, Jose [UNESP] |
author_role |
author |
author2 |
Vígaro, Renan Aparecido [UNESP] Janini, Ludimila Cardoso Zoccal [UNESP] Dal Canto, Mariabeatrice Renzini, Mario Mignini Lodde, Valentina Luciano, Alberto Maria Buratini, Jose [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Reproductive Medicine Centre University of Milan |
dc.contributor.author.fl_str_mv |
Dellaqua, Thaisy Tino [UNESP] Vígaro, Renan Aparecido [UNESP] Janini, Ludimila Cardoso Zoccal [UNESP] Dal Canto, Mariabeatrice Renzini, Mario Mignini Lodde, Valentina Luciano, Alberto Maria Buratini, Jose [UNESP] |
dc.subject.por.fl_str_mv |
AREG Bovine embryo production EGF-Like Gene expression Oocyte nuclear maturation |
topic |
AREG Bovine embryo production EGF-Like Gene expression Oocyte nuclear maturation |
description |
Oocyte in vitro maturation (IVM) is still a major challenge in human and animal assisted reproduction. Gradual instead of abrupt activation of the ovulatory cascade during IVM has been proposed to enhance nuclear-cytoplasmic synchrony and cumulus-oocyte communication, thus favoring oocyte developmental competence. Herein, we assessed the effects of neuregulin 1 (NRG1), an EGF-like factor that modulates EGFR signaling, on oocyte nuclear maturation dynamics, cumulus expansion and expression of mRNAs regulating these processes during IVM, as well as on post-IVF embryo development following AREG-stimulated IVM in cattle. In experiment 1, cumulus-oocyte complexes (COCs) were subjected to IVM with graded doses of NRG1 (1, 10 or 100 ng/mL) for 6, 9, 12, 20, and 24 h, after which oocyte nuclear status and cumulus mRNA expression were assessed. At 6 h of IVM, NRG1 at 1 ng/mL significantly decreased the percentage of GVBD (germinal vesicle breakdown) oocytes without altering later meiotic dynamics or the percentage of oocytes achieving meiosis II. In experiment 2, adding NRG1 (1 ng/mL) to the IVM medium did not affect cumulus expansion but increased the percentage of expanded and hatched blastocysts, and blastocyst total cell number following IVF/IVC. NRG1 decreased EGFR mRNA abundance while increasing NPR2 and PTX3 mRNA levels at 9 h, and TNFAIP6 mRNA abundance at 20 h of IVM. This is the first study that reports the modulatory effect of NGR1 during oocyte maturation in a mono-ovulatory species and demonstrates that this action may be applied during IVM to improve post-IVF embryo development. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:29:50Z 2023-07-29T13:29:50Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2022.10.041 Theriogenology, v. 195, p. 209-216. 0093-691X http://hdl.handle.net/11449/247930 10.1016/j.theriogenology.2022.10.041 2-s2.0-85142402416 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2022.10.041 http://hdl.handle.net/11449/247930 |
identifier_str_mv |
Theriogenology, v. 195, p. 209-216. 0093-691X 10.1016/j.theriogenology.2022.10.041 2-s2.0-85142402416 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
209-216 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964548340383744 |