Screening methylation of DNA repair genes in the oral mucosa of chronic smokers
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2018.04.017 http://hdl.handle.net/11449/179871 |
Resumo: | Objective: The aim of this study was to evaluate the epigenetic changes in the process of oral carcinogenesis by screening the methylation of repair genes in chronic smokers. Design: Two groups were formed: Group 1: 16 smokers with consumption of 20 cigarettes/day for at least 10 years; and Group 2: 10 non-smoking. Exfoliative cytology of the tongue was performed, and the extracted DNA was treated by enzymes. The PCR Array System performed methylation screening to evaluate 22 DNA repair genes, and the results were validated by RT-qPCR for each gene with methylation levels ≥10%. Results: Highest percentages of methylation were observed for MLH3 and XRCC1 genes (11–20% methylation) and in one case for MRE11A and PMS2 (>50% methylation). Statistical analysis showed significant differences in the expression of the genes MRE11A (p = 0.0002), PMS2(p = 0.0068), XRCC1 (p = 0.0080) and MLH3 (0.0057) between the two groups. Conclusion: The effects of chronic smoking on oral mucosa led to the methylation of genes MRE11A PMS2, XRCC1 and MLH3, but resulted in a reduction of gene expression of MRE11A and PMS2, which showed ≥50% methylation. These results provide evidence that smoking cause methylation and reduced expression of repair genes. |
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spelling |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokersCarcinogenesisMethylationOral mucosaRepair geneSmokingObjective: The aim of this study was to evaluate the epigenetic changes in the process of oral carcinogenesis by screening the methylation of repair genes in chronic smokers. Design: Two groups were formed: Group 1: 16 smokers with consumption of 20 cigarettes/day for at least 10 years; and Group 2: 10 non-smoking. Exfoliative cytology of the tongue was performed, and the extracted DNA was treated by enzymes. The PCR Array System performed methylation screening to evaluate 22 DNA repair genes, and the results were validated by RT-qPCR for each gene with methylation levels ≥10%. Results: Highest percentages of methylation were observed for MLH3 and XRCC1 genes (11–20% methylation) and in one case for MRE11A and PMS2 (>50% methylation). Statistical analysis showed significant differences in the expression of the genes MRE11A (p = 0.0002), PMS2(p = 0.0068), XRCC1 (p = 0.0080) and MLH3 (0.0057) between the two groups. Conclusion: The effects of chronic smoking on oral mucosa led to the methylation of genes MRE11A PMS2, XRCC1 and MLH3, but resulted in a reduction of gene expression of MRE11A and PMS2, which showed ≥50% methylation. These results provide evidence that smoking cause methylation and reduced expression of repair genes.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and TechnologyUniversity Braz Cubas, Mogi das CruzesUniversidade de Mogi das Cruzes, Mogi das CruzesAngiogenesis Research Laboratory Department of Cariology Restorative Sciences and Endodontics University of Michigan School of DentistryHeart Institute University Hospital Medical School Universidade de São PauloDepartment of Oral Pathology School of Dentistry University of Sao PauloDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and TechnologyUniversidade Estadual Paulista (Unesp)University Braz CubasUniversidade de Mogi das CruzesUniversity of Michigan School of DentistryUniversidade de São Paulo (USP)Carta, Celina Faig Lima [UNESP]Oliveira Alves, Mônica Ghislaine [UNESP]de Barros, Patrícia Pimentel [UNESP]Campos, Márcia SampaioScholz, JaquelineJorge, Antonio Olavo Cardoso [UNESP]Nunes, Fábio DaumasAlmeida, Janete Dias [UNESP]2018-12-11T17:37:06Z2018-12-11T17:37:06Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article83-87application/pdfhttp://dx.doi.org/10.1016/j.archoralbio.2018.04.017Archives of Oral Biology, v. 92, p. 83-87.1879-15060003-9969http://hdl.handle.net/11449/17987110.1016/j.archoralbio.2018.04.0172-s2.0-850471013662-s2.0-85047101366.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biology0,752info:eu-repo/semantics/openAccess2024-01-21T06:25:35Zoai:repositorio.unesp.br:11449/179871Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-21T06:25:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
title |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
spellingShingle |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers Carta, Celina Faig Lima [UNESP] Carcinogenesis Methylation Oral mucosa Repair gene Smoking |
title_short |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
title_full |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
title_fullStr |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
title_full_unstemmed |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
title_sort |
Screening methylation of DNA repair genes in the oral mucosa of chronic smokers |
author |
Carta, Celina Faig Lima [UNESP] |
author_facet |
Carta, Celina Faig Lima [UNESP] Oliveira Alves, Mônica Ghislaine [UNESP] de Barros, Patrícia Pimentel [UNESP] Campos, Márcia Sampaio Scholz, Jaqueline Jorge, Antonio Olavo Cardoso [UNESP] Nunes, Fábio Daumas Almeida, Janete Dias [UNESP] |
author_role |
author |
author2 |
Oliveira Alves, Mônica Ghislaine [UNESP] de Barros, Patrícia Pimentel [UNESP] Campos, Márcia Sampaio Scholz, Jaqueline Jorge, Antonio Olavo Cardoso [UNESP] Nunes, Fábio Daumas Almeida, Janete Dias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University Braz Cubas Universidade de Mogi das Cruzes University of Michigan School of Dentistry Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Carta, Celina Faig Lima [UNESP] Oliveira Alves, Mônica Ghislaine [UNESP] de Barros, Patrícia Pimentel [UNESP] Campos, Márcia Sampaio Scholz, Jaqueline Jorge, Antonio Olavo Cardoso [UNESP] Nunes, Fábio Daumas Almeida, Janete Dias [UNESP] |
dc.subject.por.fl_str_mv |
Carcinogenesis Methylation Oral mucosa Repair gene Smoking |
topic |
Carcinogenesis Methylation Oral mucosa Repair gene Smoking |
description |
Objective: The aim of this study was to evaluate the epigenetic changes in the process of oral carcinogenesis by screening the methylation of repair genes in chronic smokers. Design: Two groups were formed: Group 1: 16 smokers with consumption of 20 cigarettes/day for at least 10 years; and Group 2: 10 non-smoking. Exfoliative cytology of the tongue was performed, and the extracted DNA was treated by enzymes. The PCR Array System performed methylation screening to evaluate 22 DNA repair genes, and the results were validated by RT-qPCR for each gene with methylation levels ≥10%. Results: Highest percentages of methylation were observed for MLH3 and XRCC1 genes (11–20% methylation) and in one case for MRE11A and PMS2 (>50% methylation). Statistical analysis showed significant differences in the expression of the genes MRE11A (p = 0.0002), PMS2(p = 0.0068), XRCC1 (p = 0.0080) and MLH3 (0.0057) between the two groups. Conclusion: The effects of chronic smoking on oral mucosa led to the methylation of genes MRE11A PMS2, XRCC1 and MLH3, but resulted in a reduction of gene expression of MRE11A and PMS2, which showed ≥50% methylation. These results provide evidence that smoking cause methylation and reduced expression of repair genes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:37:06Z 2018-12-11T17:37:06Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2018.04.017 Archives of Oral Biology, v. 92, p. 83-87. 1879-1506 0003-9969 http://hdl.handle.net/11449/179871 10.1016/j.archoralbio.2018.04.017 2-s2.0-85047101366 2-s2.0-85047101366.pdf |
url |
http://dx.doi.org/10.1016/j.archoralbio.2018.04.017 http://hdl.handle.net/11449/179871 |
identifier_str_mv |
Archives of Oral Biology, v. 92, p. 83-87. 1879-1506 0003-9969 10.1016/j.archoralbio.2018.04.017 2-s2.0-85047101366 2-s2.0-85047101366.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology 0,752 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
83-87 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965686849601536 |