Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jas/skac089 http://hdl.handle.net/11449/241980 |
Resumo: | This study aimed to evaluate the use of total odd-chain fatty acids (OCFA) as a marker to estimate microbial nitrogen flow (MicN) and calculate the efficiency of microbial nitrogen synthesis (EMNS) in Nellore steers fed high-concentrate diets supplemented with different nitrogen supplements (NS). Ruminally and duodenally cannulated Nellore steers (n = 6; 354 ± 12 kg) were used in a 6 × 6 repeated switchback design balanced for residual effects. Treatments were arranged in a 3 × 3 factorial of three nitrogen (N) supplements (urea plus soybean meal; corn gluten meal; dried distillers' grains plus solubles) and three microbial markers (OCFA; double-labeled urea, 15N; microbial nucleic acid bases, MNAB). The total mixed ration was composed of fresh chopped sugarcane as the forage source in an 83:17 concentrate: forage ratio (dry matter basis). Linear regression was used to develop predictions of MicN from OCFA using 15N and MNAB as response variables. Microbial N flow was underestimated by the MNAB marker compared to 15N. Neither NS nor their respective interactions with the marker methods (MM) affected MicN or EMNS (P > 0.05). However, MicN was different for 15N and MNAB (P > 0.001 for both treatments). Marker methods affected EMNS in all energetic bases (total digestible carbohydrates P < 0.001; rumen-fermentable carbohydrates P < 0.001; organic matter truly degradable in the rumen P < 0.001). Equations that utilized OCFA as a regressor to predict MicN under different MM resulted in good fits of the data as observed by the coefficient of determination (R2; 15N = 0.78; MNAB = 0.69). Microbial N flow estimated from OCFA was overpredicted (15N by 7.46%; MNAB by 4.30%) compared with observed values. The OCFA model presented a small slope bias when methodological validation was applied (15N = 0.96%; MNAB = 3.90%), ensuring reliability of the proposed alternative method. Based on the conditions of this experiment, OCFA may be a suitable alternative to other methods that quantify MicN under different dietary conditions. |
id |
UNSP_a92381ce39753e973fcba3b261d0064d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/241980 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sourcesisotopesmicrobial efficiencymicrobial markersmicrobial nucleic acid basesThis study aimed to evaluate the use of total odd-chain fatty acids (OCFA) as a marker to estimate microbial nitrogen flow (MicN) and calculate the efficiency of microbial nitrogen synthesis (EMNS) in Nellore steers fed high-concentrate diets supplemented with different nitrogen supplements (NS). Ruminally and duodenally cannulated Nellore steers (n = 6; 354 ± 12 kg) were used in a 6 × 6 repeated switchback design balanced for residual effects. Treatments were arranged in a 3 × 3 factorial of three nitrogen (N) supplements (urea plus soybean meal; corn gluten meal; dried distillers' grains plus solubles) and three microbial markers (OCFA; double-labeled urea, 15N; microbial nucleic acid bases, MNAB). The total mixed ration was composed of fresh chopped sugarcane as the forage source in an 83:17 concentrate: forage ratio (dry matter basis). Linear regression was used to develop predictions of MicN from OCFA using 15N and MNAB as response variables. Microbial N flow was underestimated by the MNAB marker compared to 15N. Neither NS nor their respective interactions with the marker methods (MM) affected MicN or EMNS (P > 0.05). However, MicN was different for 15N and MNAB (P > 0.001 for both treatments). Marker methods affected EMNS in all energetic bases (total digestible carbohydrates P < 0.001; rumen-fermentable carbohydrates P < 0.001; organic matter truly degradable in the rumen P < 0.001). Equations that utilized OCFA as a regressor to predict MicN under different MM resulted in good fits of the data as observed by the coefficient of determination (R2; 15N = 0.78; MNAB = 0.69). Microbial N flow estimated from OCFA was overpredicted (15N by 7.46%; MNAB by 4.30%) compared with observed values. The OCFA model presented a small slope bias when methodological validation was applied (15N = 0.96%; MNAB = 3.90%), ensuring reliability of the proposed alternative method. Based on the conditions of this experiment, OCFA may be a suitable alternative to other methods that quantify MicN under different dietary conditions.Department of Animal Science São Paulo State University, SPDepartment of Dairy Science Virginia Polytechnic Institute and State UniversityCorporación Colombiana de Investigación Agropecuaria - AGROSAVIA CIEI Nus, AntioquíaDepartment of Physics and Biophysics São Paulo State University, SPINCT/CA-UFV-Department of Animal Science, MGDepartment of Animal Science São Paulo State University, SPDepartment of Physics and Biophysics São Paulo State University, SPUniversidade Estadual Paulista (UNESP)Virginia Polytechnic Institute and State UniversityCIEI NusUniversidade Federal de Viçosa (UFV)Campos, Letícia M. [UNESP]Souza, Vinícius C. [UNESP]Granja-Salcedo, Yury T. [UNESP]Messana, Juliana D. [UNESP]Prestegaard-Wilson, Jacquelyn M.Ganga, Maria Júlia G. [UNESP]Dias, Ana Veronica L. [UNESP]Costa, Vladimir E. [UNESP]Berchielli, Telma T. [UNESP]2023-03-02T06:28:43Z2023-03-02T06:28:43Z2022-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/jas/skac089Journal of Animal Science, v. 100, n. 5, 2022.1525-31630021-8812http://hdl.handle.net/11449/24198010.1093/jas/skac0892-s2.0-85132809282Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Animal Scienceinfo:eu-repo/semantics/openAccess2023-03-02T06:28:44Zoai:repositorio.unesp.br:11449/241980Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T06:28:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
title |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
spellingShingle |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources Campos, Letícia M. [UNESP] isotopes microbial efficiency microbial markers microbial nucleic acid bases |
title_short |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
title_full |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
title_fullStr |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
title_full_unstemmed |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
title_sort |
Odd-chain fatty acids as an alternative method to predict ruminal microbial nitrogen flow of feedlot Nellore steers fed grain-based diets supplemented with different nitrogen sources |
author |
Campos, Letícia M. [UNESP] |
author_facet |
Campos, Letícia M. [UNESP] Souza, Vinícius C. [UNESP] Granja-Salcedo, Yury T. [UNESP] Messana, Juliana D. [UNESP] Prestegaard-Wilson, Jacquelyn M. Ganga, Maria Júlia G. [UNESP] Dias, Ana Veronica L. [UNESP] Costa, Vladimir E. [UNESP] Berchielli, Telma T. [UNESP] |
author_role |
author |
author2 |
Souza, Vinícius C. [UNESP] Granja-Salcedo, Yury T. [UNESP] Messana, Juliana D. [UNESP] Prestegaard-Wilson, Jacquelyn M. Ganga, Maria Júlia G. [UNESP] Dias, Ana Veronica L. [UNESP] Costa, Vladimir E. [UNESP] Berchielli, Telma T. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Virginia Polytechnic Institute and State University CIEI Nus Universidade Federal de Viçosa (UFV) |
dc.contributor.author.fl_str_mv |
Campos, Letícia M. [UNESP] Souza, Vinícius C. [UNESP] Granja-Salcedo, Yury T. [UNESP] Messana, Juliana D. [UNESP] Prestegaard-Wilson, Jacquelyn M. Ganga, Maria Júlia G. [UNESP] Dias, Ana Veronica L. [UNESP] Costa, Vladimir E. [UNESP] Berchielli, Telma T. [UNESP] |
dc.subject.por.fl_str_mv |
isotopes microbial efficiency microbial markers microbial nucleic acid bases |
topic |
isotopes microbial efficiency microbial markers microbial nucleic acid bases |
description |
This study aimed to evaluate the use of total odd-chain fatty acids (OCFA) as a marker to estimate microbial nitrogen flow (MicN) and calculate the efficiency of microbial nitrogen synthesis (EMNS) in Nellore steers fed high-concentrate diets supplemented with different nitrogen supplements (NS). Ruminally and duodenally cannulated Nellore steers (n = 6; 354 ± 12 kg) were used in a 6 × 6 repeated switchback design balanced for residual effects. Treatments were arranged in a 3 × 3 factorial of three nitrogen (N) supplements (urea plus soybean meal; corn gluten meal; dried distillers' grains plus solubles) and three microbial markers (OCFA; double-labeled urea, 15N; microbial nucleic acid bases, MNAB). The total mixed ration was composed of fresh chopped sugarcane as the forage source in an 83:17 concentrate: forage ratio (dry matter basis). Linear regression was used to develop predictions of MicN from OCFA using 15N and MNAB as response variables. Microbial N flow was underestimated by the MNAB marker compared to 15N. Neither NS nor their respective interactions with the marker methods (MM) affected MicN or EMNS (P > 0.05). However, MicN was different for 15N and MNAB (P > 0.001 for both treatments). Marker methods affected EMNS in all energetic bases (total digestible carbohydrates P < 0.001; rumen-fermentable carbohydrates P < 0.001; organic matter truly degradable in the rumen P < 0.001). Equations that utilized OCFA as a regressor to predict MicN under different MM resulted in good fits of the data as observed by the coefficient of determination (R2; 15N = 0.78; MNAB = 0.69). Microbial N flow estimated from OCFA was overpredicted (15N by 7.46%; MNAB by 4.30%) compared with observed values. The OCFA model presented a small slope bias when methodological validation was applied (15N = 0.96%; MNAB = 3.90%), ensuring reliability of the proposed alternative method. Based on the conditions of this experiment, OCFA may be a suitable alternative to other methods that quantify MicN under different dietary conditions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01 2023-03-02T06:28:43Z 2023-03-02T06:28:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jas/skac089 Journal of Animal Science, v. 100, n. 5, 2022. 1525-3163 0021-8812 http://hdl.handle.net/11449/241980 10.1093/jas/skac089 2-s2.0-85132809282 |
url |
http://dx.doi.org/10.1093/jas/skac089 http://hdl.handle.net/11449/241980 |
identifier_str_mv |
Journal of Animal Science, v. 100, n. 5, 2022. 1525-3163 0021-8812 10.1093/jas/skac089 2-s2.0-85132809282 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Animal Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965648090038272 |