Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model
Main Author: | |
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Publication Date: | 2016 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1016/j.ijantimicag.2016.05.012 http://hdl.handle.net/11449/178250 |
Summary: | Paracoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp. and the efficacy and toxicity of AmB and itraconazole were evaluated in this model for the first time. AmB and itraconazole treatments were effective in increasing larval survival and reducing the fungal burden. The fungicidal and fungistatic effects of AmB and itraconazole, respectively, were observed in the model. Furthermore, these effects were independent of changes in haemocyte number. G. mellonella can serve as a rapid model for the screening of new antifungal compounds against Paracoccidioides and can contribute to a reduction in experimental animal numbers in the study of PCM. |
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Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella modelAlternative animal modelAntifungalsEfficacyParacoccidioidomycosisParacoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp. and the efficacy and toxicity of AmB and itraconazole were evaluated in this model for the first time. AmB and itraconazole treatments were effective in increasing larval survival and reducing the fungal burden. The fungicidal and fungistatic effects of AmB and itraconazole, respectively, were observed in the model. Furthermore, these effects were independent of changes in haemocyte number. G. mellonella can serve as a rapid model for the screening of new antifungal compounds against Paracoccidioides and can contribute to a reduction in experimental animal numbers in the study of PCM.Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), Rodovia Araraquara—Jaú Km 1Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), Rodovia Araraquara—Jaú Km 1Universidade Estadual Paulista (Unesp)de Lacorte Singulani, Junya [UNESP]Scorzoni, Liliana [UNESP]de Paula e Silva, Ana Carolina Alves [UNESP]Fusco-Almeida, Ana Marisa [UNESP]Mendes-Giannini, Maria José Soares [UNESP]2018-12-11T17:29:29Z2018-12-11T17:29:29Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article292-297application/pdfhttp://dx.doi.org/10.1016/j.ijantimicag.2016.05.012International Journal of Antimicrobial Agents, v. 48, n. 3, p. 292-297, 2016.1872-79130924-8579http://hdl.handle.net/11449/17825010.1016/j.ijantimicag.2016.05.0122-s2.0-849848301642-s2.0-84984830164.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Antimicrobial Agents1,699info:eu-repo/semantics/openAccess2023-12-05T06:15:56Zoai:repositorio.unesp.br:11449/178250Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-05T06:15:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
title |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
spellingShingle |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model de Lacorte Singulani, Junya [UNESP] Alternative animal model Antifungals Efficacy Paracoccidioidomycosis |
title_short |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
title_full |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
title_fullStr |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
title_full_unstemmed |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
title_sort |
Evaluation of the efficacy of antifungal drugs against Paracoccidioides brasiliensis and Paracoccidioides lutzii in a Galleria mellonella model |
author |
de Lacorte Singulani, Junya [UNESP] |
author_facet |
de Lacorte Singulani, Junya [UNESP] Scorzoni, Liliana [UNESP] de Paula e Silva, Ana Carolina Alves [UNESP] Fusco-Almeida, Ana Marisa [UNESP] Mendes-Giannini, Maria José Soares [UNESP] |
author_role |
author |
author2 |
Scorzoni, Liliana [UNESP] de Paula e Silva, Ana Carolina Alves [UNESP] Fusco-Almeida, Ana Marisa [UNESP] Mendes-Giannini, Maria José Soares [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Lacorte Singulani, Junya [UNESP] Scorzoni, Liliana [UNESP] de Paula e Silva, Ana Carolina Alves [UNESP] Fusco-Almeida, Ana Marisa [UNESP] Mendes-Giannini, Maria José Soares [UNESP] |
dc.subject.por.fl_str_mv |
Alternative animal model Antifungals Efficacy Paracoccidioidomycosis |
topic |
Alternative animal model Antifungals Efficacy Paracoccidioidomycosis |
description |
Paracoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp. and the efficacy and toxicity of AmB and itraconazole were evaluated in this model for the first time. AmB and itraconazole treatments were effective in increasing larval survival and reducing the fungal burden. The fungicidal and fungistatic effects of AmB and itraconazole, respectively, were observed in the model. Furthermore, these effects were independent of changes in haemocyte number. G. mellonella can serve as a rapid model for the screening of new antifungal compounds against Paracoccidioides and can contribute to a reduction in experimental animal numbers in the study of PCM. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:29:29Z 2018-12-11T17:29:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijantimicag.2016.05.012 International Journal of Antimicrobial Agents, v. 48, n. 3, p. 292-297, 2016. 1872-7913 0924-8579 http://hdl.handle.net/11449/178250 10.1016/j.ijantimicag.2016.05.012 2-s2.0-84984830164 2-s2.0-84984830164.pdf |
url |
http://dx.doi.org/10.1016/j.ijantimicag.2016.05.012 http://hdl.handle.net/11449/178250 |
identifier_str_mv |
International Journal of Antimicrobial Agents, v. 48, n. 3, p. 292-297, 2016. 1872-7913 0924-8579 10.1016/j.ijantimicag.2016.05.012 2-s2.0-84984830164 2-s2.0-84984830164.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Antimicrobial Agents 1,699 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
292-297 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965176748834816 |