Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/2013.January.16.8 http://hdl.handle.net/11449/74388 |
Resumo: | We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20′, 47°17′W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as fresh and 36 as non-fresh. DNA was extracted using the QIAamp® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples. Five microsatellite loci were amplified by PCR; success in amplification varied according to locus size and sample age. Successful amplifications were achieved in 10/16 of the fresh and in 13/36 of the non-fresh samples; a negative correlation (R = -0.82) was found between successful amplification and locus size. Amplification of the amelogenin locus was successful in 22 of the 52 samples. The difficulty of amplifying nuclear loci in DNA samples extractedfrom feces collected in the field was evident. Some methodological improvements, including collecting fresh samples, selecting primers for shorter loci and quantifying the extracted DNA by real-time PCR, are suggested to increase amplification success in future studies. © FUNPEC-RP. |
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Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)Cytochrome bDetection dogFecal DNAMicrosatelliteMolecular ecologyamelogenincytochrome bcytochrome cmicrosatellite DNAmitochondrial DNAmolecular marker3' untranslated region5' untranslated regionageanimal experimentBrazilcontrolled studycytochrome b genedeerDNA extractionfeces analysisfemalegene locusgenotypegeographic distributionmaleMazama americananonhumanreal time polymerase chain reactionsequence analysiswild typeWe tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20′, 47°17′W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as fresh and 36 as non-fresh. DNA was extracted using the QIAamp® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples. Five microsatellite loci were amplified by PCR; success in amplification varied according to locus size and sample age. Successful amplifications were achieved in 10/16 of the fresh and in 13/36 of the non-fresh samples; a negative correlation (R = -0.82) was found between successful amplification and locus size. Amplification of the amelogenin locus was successful in 22 of the 52 samples. The difficulty of amplifying nuclear loci in DNA samples extractedfrom feces collected in the field was evident. Some methodological improvements, including collecting fresh samples, selecting primers for shorter loci and quantifying the extracted DNA by real-time PCR, are suggested to increase amplification success in future studies. © FUNPEC-RP.Núcleo de Pesquisa e Conservação de Cervídeos Universidade Estadual Paulista 'Júlio de Mesquita Filho', Jaboticabal, SPNúcleo de Pesquisa e Conservação de Cervídeos Universidade Estadual Paulista 'Júlio de Mesquita Filho', Jaboticabal, SPUniversidade Estadual Paulista (Unesp)Oliveira, M. L. [UNESP]Duarte, J. M B [UNESP]2014-05-27T11:28:10Z2014-05-27T11:28:10Z2013-01-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article44-52application/pdfhttp://dx.doi.org/10.4238/2013.January.16.8Genetics and Molecular Research, v. 12, n. 1, p. 44-52, 2013.1676-5680http://hdl.handle.net/11449/7438810.4238/2013.January.16.8WOS:0003188644000062-s2.0-848729225392-s2.0-84872922539.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-09T06:18:43Zoai:repositorio.unesp.br:11449/74388Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-09T06:18:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
title |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
spellingShingle |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) Oliveira, M. L. [UNESP] Cytochrome b Detection dog Fecal DNA Microsatellite Molecular ecology amelogenin cytochrome b cytochrome c microsatellite DNA mitochondrial DNA molecular marker 3' untranslated region 5' untranslated region age animal experiment Brazil controlled study cytochrome b gene deer DNA extraction feces analysis female gene locus genotype geographic distribution male Mazama americana nonhuman real time polymerase chain reaction sequence analysis wild type |
title_short |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
title_full |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
title_fullStr |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
title_full_unstemmed |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
title_sort |
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae) |
author |
Oliveira, M. L. [UNESP] |
author_facet |
Oliveira, M. L. [UNESP] Duarte, J. M B [UNESP] |
author_role |
author |
author2 |
Duarte, J. M B [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Oliveira, M. L. [UNESP] Duarte, J. M B [UNESP] |
dc.subject.por.fl_str_mv |
Cytochrome b Detection dog Fecal DNA Microsatellite Molecular ecology amelogenin cytochrome b cytochrome c microsatellite DNA mitochondrial DNA molecular marker 3' untranslated region 5' untranslated region age animal experiment Brazil controlled study cytochrome b gene deer DNA extraction feces analysis female gene locus genotype geographic distribution male Mazama americana nonhuman real time polymerase chain reaction sequence analysis wild type |
topic |
Cytochrome b Detection dog Fecal DNA Microsatellite Molecular ecology amelogenin cytochrome b cytochrome c microsatellite DNA mitochondrial DNA molecular marker 3' untranslated region 5' untranslated region age animal experiment Brazil controlled study cytochrome b gene deer DNA extraction feces analysis female gene locus genotype geographic distribution male Mazama americana nonhuman real time polymerase chain reaction sequence analysis wild type |
description |
We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20′, 47°17′W), with the help of a detection dog; then, stored in ethanol and georeferenced. Among these samples 16 were classified as fresh and 36 as non-fresh. DNA was extracted using the QIAamp® DNA Stool Mini Kit. Mitochondrial loci were amplified in 49 of the 52 samples. Five microsatellite loci were amplified by PCR; success in amplification varied according to locus size and sample age. Successful amplifications were achieved in 10/16 of the fresh and in 13/36 of the non-fresh samples; a negative correlation (R = -0.82) was found between successful amplification and locus size. Amplification of the amelogenin locus was successful in 22 of the 52 samples. The difficulty of amplifying nuclear loci in DNA samples extractedfrom feces collected in the field was evident. Some methodological improvements, including collecting fresh samples, selecting primers for shorter loci and quantifying the extracted DNA by real-time PCR, are suggested to increase amplification success in future studies. © FUNPEC-RP. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-16 2014-05-27T11:28:10Z 2014-05-27T11:28:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/2013.January.16.8 Genetics and Molecular Research, v. 12, n. 1, p. 44-52, 2013. 1676-5680 http://hdl.handle.net/11449/74388 10.4238/2013.January.16.8 WOS:000318864400006 2-s2.0-84872922539 2-s2.0-84872922539.pdf |
url |
http://dx.doi.org/10.4238/2013.January.16.8 http://hdl.handle.net/11449/74388 |
identifier_str_mv |
Genetics and Molecular Research, v. 12, n. 1, p. 44-52, 2013. 1676-5680 10.4238/2013.January.16.8 WOS:000318864400006 2-s2.0-84872922539 2-s2.0-84872922539.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
44-52 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789947558625280 |