Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue
Main Author: | |
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Publication Date: | 1998 |
Other Authors: | , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1002/(SICI)1097-0134(19980301)30:4<442 http://hdl.handle.net/11449/224091 |
Summary: | Bothropstoxin I (BthTX-I) from the venom of Bothrops jararacussu is a myotoxic phospholipase A2 (PLA2) homologue which, although catalytically inactive due to an Asp49→Lys substitution, disrupts the integrity of lipid membranes by a Ca2+-independent mechanism. The crystal structures of two dimeric forms of BthTX-I which diffract X-rays to resolutions of 3.1 and 2.1 Å have been determined. The monomers in both structures are related by an almost perfect twofold axis of rotation and the dimer interfaces are defined by contacts between the N-terminal α-helical regions and the tips of the β- wings of partner monomers. Significant differences in the relative orientation of the monomers in the two crystal forms results in 'open'and 'closed' dimer conformations. Spectroscopic investigations of BthTX-I in solution have correlated these conformational differences with changes in the intrinsic fluorescence emission of the single tryptophan residues located at the dimer interface. The possible relevance of this structural transition in the Ca2+-independent membrane damaging activity is discussed. |
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Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologueProtein-membrane interactionQuaternary structural changeSpectroscopyVenom toxinX-ray diffractionBothropstoxin I (BthTX-I) from the venom of Bothrops jararacussu is a myotoxic phospholipase A2 (PLA2) homologue which, although catalytically inactive due to an Asp49→Lys substitution, disrupts the integrity of lipid membranes by a Ca2+-independent mechanism. The crystal structures of two dimeric forms of BthTX-I which diffract X-rays to resolutions of 3.1 and 2.1 Å have been determined. The monomers in both structures are related by an almost perfect twofold axis of rotation and the dimer interfaces are defined by contacts between the N-terminal α-helical regions and the tips of the β- wings of partner monomers. Significant differences in the relative orientation of the monomers in the two crystal forms results in 'open'and 'closed' dimer conformations. Spectroscopic investigations of BthTX-I in solution have correlated these conformational differences with changes in the intrinsic fluorescence emission of the single tryptophan residues located at the dimer interface. The possible relevance of this structural transition in the Ca2+-independent membrane damaging activity is discussed.Institute of Physics (IFSC) USP, São Carlos-SPDepartment of Biochemistry Faculty of Medicine USP, Ribeirão Preto-SPDepartment of Physics IBILCE-UNESP, Sao Jose do Rio Preto-SPDepartment of Biochemistry FMRP-USP, Avenida Bandeirantes 3900, CEP 14049-900, Ribeirao Preto-SPDepartment of Physics IBILCE-UNESP, Sao Jose do Rio Preto-SPUniversidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Da Silva Giotto, M. T.Garratt, R. C.Oliva, G.Mascarenhas, Y. P.Giglio, J. R.Cintra, A. C.O.De Azevedo, W. F. [UNESP]Arni, R. K. [UNESP]Ward, R. J. [UNESP]2022-04-28T19:54:34Z2022-04-28T19:54:34Z1998-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article442-454http://dx.doi.org/10.1002/(SICI)1097-0134(19980301)30:4<442Proteins: Structure, Function and Genetics, v. 30, n. 4, p. 442-454, 1998.0887-3585http://hdl.handle.net/11449/22409110.1002/(SICI)1097-0134(19980301)30:4<4422-s2.0-0032031373Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProteins: Structure, Function and Geneticsinfo:eu-repo/semantics/openAccess2022-04-28T19:54:35Zoai:repositorio.unesp.br:11449/224091Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:54:35Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
title |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
spellingShingle |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue Da Silva Giotto, M. T. Protein-membrane interaction Quaternary structural change Spectroscopy Venom toxin X-ray diffraction |
title_short |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
title_full |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
title_fullStr |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
title_full_unstemmed |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
title_sort |
Crystallographic and spectroscopic characterization of a molecular hinge: Conformational changes in Bothropstoxin I, a dimeric Lys49- phospholipase A2 homologue |
author |
Da Silva Giotto, M. T. |
author_facet |
Da Silva Giotto, M. T. Garratt, R. C. Oliva, G. Mascarenhas, Y. P. Giglio, J. R. Cintra, A. C.O. De Azevedo, W. F. [UNESP] Arni, R. K. [UNESP] Ward, R. J. [UNESP] |
author_role |
author |
author2 |
Garratt, R. C. Oliva, G. Mascarenhas, Y. P. Giglio, J. R. Cintra, A. C.O. De Azevedo, W. F. [UNESP] Arni, R. K. [UNESP] Ward, R. J. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Da Silva Giotto, M. T. Garratt, R. C. Oliva, G. Mascarenhas, Y. P. Giglio, J. R. Cintra, A. C.O. De Azevedo, W. F. [UNESP] Arni, R. K. [UNESP] Ward, R. J. [UNESP] |
dc.subject.por.fl_str_mv |
Protein-membrane interaction Quaternary structural change Spectroscopy Venom toxin X-ray diffraction |
topic |
Protein-membrane interaction Quaternary structural change Spectroscopy Venom toxin X-ray diffraction |
description |
Bothropstoxin I (BthTX-I) from the venom of Bothrops jararacussu is a myotoxic phospholipase A2 (PLA2) homologue which, although catalytically inactive due to an Asp49→Lys substitution, disrupts the integrity of lipid membranes by a Ca2+-independent mechanism. The crystal structures of two dimeric forms of BthTX-I which diffract X-rays to resolutions of 3.1 and 2.1 Å have been determined. The monomers in both structures are related by an almost perfect twofold axis of rotation and the dimer interfaces are defined by contacts between the N-terminal α-helical regions and the tips of the β- wings of partner monomers. Significant differences in the relative orientation of the monomers in the two crystal forms results in 'open'and 'closed' dimer conformations. Spectroscopic investigations of BthTX-I in solution have correlated these conformational differences with changes in the intrinsic fluorescence emission of the single tryptophan residues located at the dimer interface. The possible relevance of this structural transition in the Ca2+-independent membrane damaging activity is discussed. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-03-01 2022-04-28T19:54:34Z 2022-04-28T19:54:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/(SICI)1097-0134(19980301)30:4<442 Proteins: Structure, Function and Genetics, v. 30, n. 4, p. 442-454, 1998. 0887-3585 http://hdl.handle.net/11449/224091 10.1002/(SICI)1097-0134(19980301)30:4<442 2-s2.0-0032031373 |
url |
http://dx.doi.org/10.1002/(SICI)1097-0134(19980301)30:4<442 http://hdl.handle.net/11449/224091 |
identifier_str_mv |
Proteins: Structure, Function and Genetics, v. 30, n. 4, p. 442-454, 1998. 0887-3585 10.1002/(SICI)1097-0134(19980301)30:4<442 2-s2.0-0032031373 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Proteins: Structure, Function and Genetics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
442-454 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964720438968320 |