Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jip.2020.107525 http://hdl.handle.net/11449/210057 |
Resumo: | The growing spread of dengue, chikungunya and Zika viruses demand the development of new and environmentally safe control methods for their vector, the mosquito Aedes aegypti. This study aims to find novel larvicidal agents from mutualistic (endophytic and rhizospheric) or edaphic bacteria that have no action against nontarget organisms. Eleven out of the 254 bacterial strains tested were able to kill Ae. aegypti larvae. Larvicidal activity did not depend on presence of cells, since culture supernatants or crude lipopeptide extracts (CLEs) killed the larvae. Bacillus safensis BacI67 and Bacillus paranthracis C21 supernatants were the best performing supernatants, displaying the lowest lethal concentrations (LC50 = 31.11 mu L/mL and 45.84 mu L/mL, respectively). Bacillus velezensis B64a and Bacillus velezensis B15 produced the best performing CLEs (LC50 = 0.11 mg/mL and 0.12 mg/mL, respectively). Mass spectrometry analysis of CLEs detected a mixture of surfactins, iturins, and fengycins. The samples tested were weakly- or non-toxic to mammalian cells (RAW 264.7 macrophages and VERO cells) and non-target organisms (Caenorhabditis elegans, Galleria mellonella, Scenedesmus obliques, and Tetrahymena pyriformis) - especially B. velezensis B15 CLE. The biosynthetic gene clusters related to secondary metabolism identified by whole genome sequencing of the four best performing bacteria strains revealed clusters for bacteriocin, beta-lactone, lanthipeptide, non-ribosomal peptide synthetases, polyketide synthases (PKS), siderophores, T3PKS, type 1 PKS-like, terpenes, thiopeptides, and trans-AT-PKS. Purification of lipopeptides may clarify the mechanisms by which these extracts kill Ae. aegypti larvae. |
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Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target speciesBacillus safensisBacillus paranthracisBacillus velezensisLipopeptideGenome miningThe growing spread of dengue, chikungunya and Zika viruses demand the development of new and environmentally safe control methods for their vector, the mosquito Aedes aegypti. This study aims to find novel larvicidal agents from mutualistic (endophytic and rhizospheric) or edaphic bacteria that have no action against nontarget organisms. Eleven out of the 254 bacterial strains tested were able to kill Ae. aegypti larvae. Larvicidal activity did not depend on presence of cells, since culture supernatants or crude lipopeptide extracts (CLEs) killed the larvae. Bacillus safensis BacI67 and Bacillus paranthracis C21 supernatants were the best performing supernatants, displaying the lowest lethal concentrations (LC50 = 31.11 mu L/mL and 45.84 mu L/mL, respectively). Bacillus velezensis B64a and Bacillus velezensis B15 produced the best performing CLEs (LC50 = 0.11 mg/mL and 0.12 mg/mL, respectively). Mass spectrometry analysis of CLEs detected a mixture of surfactins, iturins, and fengycins. The samples tested were weakly- or non-toxic to mammalian cells (RAW 264.7 macrophages and VERO cells) and non-target organisms (Caenorhabditis elegans, Galleria mellonella, Scenedesmus obliques, and Tetrahymena pyriformis) - especially B. velezensis B15 CLE. The biosynthetic gene clusters related to secondary metabolism identified by whole genome sequencing of the four best performing bacteria strains revealed clusters for bacteriocin, beta-lactone, lanthipeptide, non-ribosomal peptide synthetases, polyketide synthases (PKS), siderophores, T3PKS, type 1 PKS-like, terpenes, thiopeptides, and trans-AT-PKS. Purification of lipopeptides may clarify the mechanisms by which these extracts kill Ae. aegypti larvae.Fundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT, Mato Grosso, MT, Brazil)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Mato Grosso, Dept Bot & Ecol, Av Fernando Correa da Costa 2367, BR-78060900 Cuiaba, BrazilUniv Fed Vicosa, Dept Bioquim & Biol Mol, Vicosa, MG, BrazilEmbrapa Amazonia Ocident, Manaus, Amazonas, BrazilUniv Estadual Paulista, Ctr Inovacao Biodiversidade & Farmacos, Inst Quim, Araraquara, SP, BrazilUniv Estadual Paulista, Ctr Inovacao Biodiversidade & Farmacos, Inst Quim, Araraquara, SP, BrazilFundacao de Amparo a Pesquisa do Estado de Mato Grosso (FAPEMAT, Mato Grosso, MT, Brazil): 0415241/2016CNPq: 307288/2018-8Elsevier B.V.Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Federal de Viçosa (UFV)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Falqueto, Silvia AltoePitaluga, Bruno FariaSousa, Janaina Rosa deTarganski, Sabrina KetrinCampos, Mateus GandraOliveira Mendes, Tiago Antonio deSilva, Gilvan Ferreira daSiqueira Silva, Dulce Helena [UNESP]Soares, Marcos Antonio2021-06-25T12:38:21Z2021-06-25T12:38:21Z2021-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10http://dx.doi.org/10.1016/j.jip.2020.107525Journal Of Invertebrate Pathology. San Diego: Academic Press Inc Elsevier Science, v. 179, 10 p., 2021.0022-2011http://hdl.handle.net/11449/21005710.1016/j.jip.2020.107525WOS:000620238500001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Invertebrate Pathologyinfo:eu-repo/semantics/openAccess2021-10-23T19:50:16Zoai:repositorio.unesp.br:11449/210057Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
title |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
spellingShingle |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species Falqueto, Silvia Altoe Bacillus safensis Bacillus paranthracis Bacillus velezensis Lipopeptide Genome mining |
title_short |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
title_full |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
title_fullStr |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
title_full_unstemmed |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
title_sort |
Bacillus spp. metabolites are effective in eradicating Aedes aegypti (Diptera: Culicidae) larvae with low toxicity to non-target species |
author |
Falqueto, Silvia Altoe |
author_facet |
Falqueto, Silvia Altoe Pitaluga, Bruno Faria Sousa, Janaina Rosa de Targanski, Sabrina Ketrin Campos, Mateus Gandra Oliveira Mendes, Tiago Antonio de Silva, Gilvan Ferreira da Siqueira Silva, Dulce Helena [UNESP] Soares, Marcos Antonio |
author_role |
author |
author2 |
Pitaluga, Bruno Faria Sousa, Janaina Rosa de Targanski, Sabrina Ketrin Campos, Mateus Gandra Oliveira Mendes, Tiago Antonio de Silva, Gilvan Ferreira da Siqueira Silva, Dulce Helena [UNESP] Soares, Marcos Antonio |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Federal de Viçosa (UFV) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Falqueto, Silvia Altoe Pitaluga, Bruno Faria Sousa, Janaina Rosa de Targanski, Sabrina Ketrin Campos, Mateus Gandra Oliveira Mendes, Tiago Antonio de Silva, Gilvan Ferreira da Siqueira Silva, Dulce Helena [UNESP] Soares, Marcos Antonio |
dc.subject.por.fl_str_mv |
Bacillus safensis Bacillus paranthracis Bacillus velezensis Lipopeptide Genome mining |
topic |
Bacillus safensis Bacillus paranthracis Bacillus velezensis Lipopeptide Genome mining |
description |
The growing spread of dengue, chikungunya and Zika viruses demand the development of new and environmentally safe control methods for their vector, the mosquito Aedes aegypti. This study aims to find novel larvicidal agents from mutualistic (endophytic and rhizospheric) or edaphic bacteria that have no action against nontarget organisms. Eleven out of the 254 bacterial strains tested were able to kill Ae. aegypti larvae. Larvicidal activity did not depend on presence of cells, since culture supernatants or crude lipopeptide extracts (CLEs) killed the larvae. Bacillus safensis BacI67 and Bacillus paranthracis C21 supernatants were the best performing supernatants, displaying the lowest lethal concentrations (LC50 = 31.11 mu L/mL and 45.84 mu L/mL, respectively). Bacillus velezensis B64a and Bacillus velezensis B15 produced the best performing CLEs (LC50 = 0.11 mg/mL and 0.12 mg/mL, respectively). Mass spectrometry analysis of CLEs detected a mixture of surfactins, iturins, and fengycins. The samples tested were weakly- or non-toxic to mammalian cells (RAW 264.7 macrophages and VERO cells) and non-target organisms (Caenorhabditis elegans, Galleria mellonella, Scenedesmus obliques, and Tetrahymena pyriformis) - especially B. velezensis B15 CLE. The biosynthetic gene clusters related to secondary metabolism identified by whole genome sequencing of the four best performing bacteria strains revealed clusters for bacteriocin, beta-lactone, lanthipeptide, non-ribosomal peptide synthetases, polyketide synthases (PKS), siderophores, T3PKS, type 1 PKS-like, terpenes, thiopeptides, and trans-AT-PKS. Purification of lipopeptides may clarify the mechanisms by which these extracts kill Ae. aegypti larvae. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T12:38:21Z 2021-06-25T12:38:21Z 2021-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jip.2020.107525 Journal Of Invertebrate Pathology. San Diego: Academic Press Inc Elsevier Science, v. 179, 10 p., 2021. 0022-2011 http://hdl.handle.net/11449/210057 10.1016/j.jip.2020.107525 WOS:000620238500001 |
url |
http://dx.doi.org/10.1016/j.jip.2020.107525 http://hdl.handle.net/11449/210057 |
identifier_str_mv |
Journal Of Invertebrate Pathology. San Diego: Academic Press Inc Elsevier Science, v. 179, 10 p., 2021. 0022-2011 10.1016/j.jip.2020.107525 WOS:000620238500001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Invertebrate Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964498176507904 |