Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2016001200007 http://hdl.handle.net/11449/169526 |
Resumo: | Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70's new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified, CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central--West region of Sao Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination testand those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in Sao Paulo region. |
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Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, BrazilCanine parvovirusDogsPCRViral typeSince the first isolation of canine parvovirus type 2 (CPV-2) in late 70's new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified, CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central--West region of Sao Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination testand those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in Sao Paulo region.Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nFaculdade de Medicina Veterinária Universidade de Santo AmaroFaculdade de Medicina Veterinária e Zootecnia Universidade de São PauloFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/nUniversidade Estadual Paulista (Unesp)Universidade de Santo AmaroUniversidade de São Paulo (USP)Monteiro, Kyssia [UNESP]Allendorf, Susan D. [UNESP]Vicente, Acácia F. [UNESP]Appolinário, Camila M. [UNESP]Peres, Marina G. [UNESP]Cortez, AdrianaHeinemann, Marcos B.Megid, Jane [UNESP]2018-12-11T16:46:16Z2018-12-11T16:46:16Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1181-1185application/pdfhttp://dx.doi.org/10.1590/S0100-736X2016001200007Pesquisa Veterinaria Brasileira, v. 36, n. 12, p. 1181-1185, 2016.1678-51500100-736Xhttp://hdl.handle.net/11449/16952610.1590/S0100-736X2016001200007S0100-736X20160012011812-s2.0-85014769011S0100-736X2016001201181.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-10-30T06:06:22Zoai:repositorio.unesp.br:11449/169526Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-30T06:06:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
title |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
spellingShingle |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil Monteiro, Kyssia [UNESP] Canine parvovirus Dogs PCR Viral type |
title_short |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
title_full |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
title_fullStr |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
title_full_unstemmed |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
title_sort |
Viral type characterization and clinical aspects of canine parvovirus in naturally infected dogs in Sao Paulo State, Brazil |
author |
Monteiro, Kyssia [UNESP] |
author_facet |
Monteiro, Kyssia [UNESP] Allendorf, Susan D. [UNESP] Vicente, Acácia F. [UNESP] Appolinário, Camila M. [UNESP] Peres, Marina G. [UNESP] Cortez, Adriana Heinemann, Marcos B. Megid, Jane [UNESP] |
author_role |
author |
author2 |
Allendorf, Susan D. [UNESP] Vicente, Acácia F. [UNESP] Appolinário, Camila M. [UNESP] Peres, Marina G. [UNESP] Cortez, Adriana Heinemann, Marcos B. Megid, Jane [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de Santo Amaro Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Monteiro, Kyssia [UNESP] Allendorf, Susan D. [UNESP] Vicente, Acácia F. [UNESP] Appolinário, Camila M. [UNESP] Peres, Marina G. [UNESP] Cortez, Adriana Heinemann, Marcos B. Megid, Jane [UNESP] |
dc.subject.por.fl_str_mv |
Canine parvovirus Dogs PCR Viral type |
topic |
Canine parvovirus Dogs PCR Viral type |
description |
Since the first isolation of canine parvovirus type 2 (CPV-2) in late 70's new virus types as CPV-2a and CPV-2b have been emerged and becoming prevalent in natural canine population and more recently, a third subtype was identified, CPV-2c. The main purpose of this study was to detect and characterize canine parvovirus currently present in Central--West region of Sao Paulo state, in Brazil. Fecal samples were collected of vaccinated and non-vaccinated dogs, clinically suspected of having CPV infection brought to the Infectious Diseases Service, Veterinary Hospital of FMVZ-UNESP. All samples (n=30) were screening for canine parvovirus through hemagglutination testand those resulting as positive (n=20) were submitted to PCR and the products were subsequently sequenced for subtype characterization. Results were tested for association with age, hematological values, viral hemagglutination titers in the feces, vaccination status and survival. Leukopenia was found in all animals, death occurred in 30% of unvaccinated dogs and in 42% of vaccinated ones. In a total of 20 positive sequenced samples, 18 were classified as CPV-2b, one as CPV-2c, and one as CPV-2a, being CPV2a and CPV2c detected in unvaccinated puppies. Compared to the reference samples amino acid change at position 426 in those circling virus was identified. The study results demonstrate the predominance of CPV-2b and the presence of CPV-2a and CPV-2c in naturally infected, vaccinated and unvaccinated dogs in in Sao Paulo region. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T16:46:16Z 2018-12-11T16:46:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2016001200007 Pesquisa Veterinaria Brasileira, v. 36, n. 12, p. 1181-1185, 2016. 1678-5150 0100-736X http://hdl.handle.net/11449/169526 10.1590/S0100-736X2016001200007 S0100-736X2016001201181 2-s2.0-85014769011 S0100-736X2016001201181.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2016001200007 http://hdl.handle.net/11449/169526 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 36, n. 12, p. 1181-1185, 2016. 1678-5150 0100-736X 10.1590/S0100-736X2016001200007 S0100-736X2016001201181 2-s2.0-85014769011 S0100-736X2016001201181.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1181-1185 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789554601623552 |