Crotoxin-induced behavioral effects in rats
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/224032 |
Resumo: | Crotoxin is the major component of Crotalus durissus terrificus venom. In view of the presence of high-affinity specific binding sites for crotoxin in the brain, the objective of this work was to investigate whether crotoxin induces behavioral effects in the open-field and hole-board tests. Adult male Wistar rats (180-220 g) treated with crotoxin, 100, 250 and 500 μg/kg, ip, administered 2 h before the test, presented statistically significant behavioral alterations (ANOVA for one-way classification complemented with Dunnet test, P < 0.05). In the open-field test, 250 and 500 μg/kg of crotoxin increased freezing (from 3.22 sec to 10.75 sec and 11.2 sec) and grooming (from 13.44 sec to 22.75 sec and 21.22 sec) and decreased ambulation (from 64.8 to 39.38 and 45.8). The dose of 500 μg/kg also decreased rearing (from 24.9 to 17.5). In the hole-board test, 500 μg/kg of crotoxin decreased head-dip count (from 6.33 to 4.00). All the crotoxin-induced behavioral effects were antagonized by an anxiolytic dose of diazepam (1.5 mg/kg, ip, 30 min before the tests). These results show that crotoxin reduced open-field activity and exploratory behavior as well. We suggest that these effects express an increased emotional state induced by this toxin. |
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Crotoxin-induced behavioral effects in ratsCrotalus durissus terrificus venomcrotoxinemotionalityhole-boardopen-fieldCrotoxin is the major component of Crotalus durissus terrificus venom. In view of the presence of high-affinity specific binding sites for crotoxin in the brain, the objective of this work was to investigate whether crotoxin induces behavioral effects in the open-field and hole-board tests. Adult male Wistar rats (180-220 g) treated with crotoxin, 100, 250 and 500 μg/kg, ip, administered 2 h before the test, presented statistically significant behavioral alterations (ANOVA for one-way classification complemented with Dunnet test, P < 0.05). In the open-field test, 250 and 500 μg/kg of crotoxin increased freezing (from 3.22 sec to 10.75 sec and 11.2 sec) and grooming (from 13.44 sec to 22.75 sec and 21.22 sec) and decreased ambulation (from 64.8 to 39.38 and 45.8). The dose of 500 μg/kg also decreased rearing (from 24.9 to 17.5). In the hole-board test, 500 μg/kg of crotoxin decreased head-dip count (from 6.33 to 4.00). All the crotoxin-induced behavioral effects were antagonized by an anxiolytic dose of diazepam (1.5 mg/kg, ip, 30 min before the tests). These results show that crotoxin reduced open-field activity and exploratory behavior as well. We suggest that these effects express an increased emotional state induced by this toxin.Departamento de Farmacologia Universidade Estadual Paulista, 18618-000 Botucatu, SPDepto. de Bioestatística Universidade Estadual Paulista, 18618-000 Botucatu, SPDivisão de Radiobiologia Inst. de Pesq. Energeticas e Nucl., 05508-000 São Paulo, SPDepartamento de Farmacologia Universidade Estadual Paulista, Caixa Postal 520, 18618-000 Botucatu, SPDepartamento de Farmacologia Universidade Estadual Paulista, 18618-000 Botucatu, SPDepto. de Bioestatística Universidade Estadual Paulista, 18618-000 Botucatu, SPDepartamento de Farmacologia Universidade Estadual Paulista, Caixa Postal 520, 18618-000 Botucatu, SPUniversidade Estadual Paulista (UNESP)Inst. de Pesq. Energeticas e Nucl.Moreira, E. G. [UNESP]Nascimento, N.Rosa, G. J.M. [UNESP]Rogero, J. R.Vassilieff, V. S. [UNESP]2022-04-28T19:54:23Z2022-04-28T19:54:23Z1996-06-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article629-632Brazilian Journal of Medical and Biological Research, v. 29, n. 5, p. 629-632, 1996.0100-879Xhttp://hdl.handle.net/11449/2240322-s2.0-0030003217Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/openAccess2022-04-28T19:54:23Zoai:repositorio.unesp.br:11449/224032Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T19:54:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Crotoxin-induced behavioral effects in rats |
title |
Crotoxin-induced behavioral effects in rats |
spellingShingle |
Crotoxin-induced behavioral effects in rats Moreira, E. G. [UNESP] Crotalus durissus terrificus venom crotoxin emotionality hole-board open-field |
title_short |
Crotoxin-induced behavioral effects in rats |
title_full |
Crotoxin-induced behavioral effects in rats |
title_fullStr |
Crotoxin-induced behavioral effects in rats |
title_full_unstemmed |
Crotoxin-induced behavioral effects in rats |
title_sort |
Crotoxin-induced behavioral effects in rats |
author |
Moreira, E. G. [UNESP] |
author_facet |
Moreira, E. G. [UNESP] Nascimento, N. Rosa, G. J.M. [UNESP] Rogero, J. R. Vassilieff, V. S. [UNESP] |
author_role |
author |
author2 |
Nascimento, N. Rosa, G. J.M. [UNESP] Rogero, J. R. Vassilieff, V. S. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Inst. de Pesq. Energeticas e Nucl. |
dc.contributor.author.fl_str_mv |
Moreira, E. G. [UNESP] Nascimento, N. Rosa, G. J.M. [UNESP] Rogero, J. R. Vassilieff, V. S. [UNESP] |
dc.subject.por.fl_str_mv |
Crotalus durissus terrificus venom crotoxin emotionality hole-board open-field |
topic |
Crotalus durissus terrificus venom crotoxin emotionality hole-board open-field |
description |
Crotoxin is the major component of Crotalus durissus terrificus venom. In view of the presence of high-affinity specific binding sites for crotoxin in the brain, the objective of this work was to investigate whether crotoxin induces behavioral effects in the open-field and hole-board tests. Adult male Wistar rats (180-220 g) treated with crotoxin, 100, 250 and 500 μg/kg, ip, administered 2 h before the test, presented statistically significant behavioral alterations (ANOVA for one-way classification complemented with Dunnet test, P < 0.05). In the open-field test, 250 and 500 μg/kg of crotoxin increased freezing (from 3.22 sec to 10.75 sec and 11.2 sec) and grooming (from 13.44 sec to 22.75 sec and 21.22 sec) and decreased ambulation (from 64.8 to 39.38 and 45.8). The dose of 500 μg/kg also decreased rearing (from 24.9 to 17.5). In the hole-board test, 500 μg/kg of crotoxin decreased head-dip count (from 6.33 to 4.00). All the crotoxin-induced behavioral effects were antagonized by an anxiolytic dose of diazepam (1.5 mg/kg, ip, 30 min before the tests). These results show that crotoxin reduced open-field activity and exploratory behavior as well. We suggest that these effects express an increased emotional state induced by this toxin. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-06-04 2022-04-28T19:54:23Z 2022-04-28T19:54:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Brazilian Journal of Medical and Biological Research, v. 29, n. 5, p. 629-632, 1996. 0100-879X http://hdl.handle.net/11449/224032 2-s2.0-0030003217 |
identifier_str_mv |
Brazilian Journal of Medical and Biological Research, v. 29, n. 5, p. 629-632, 1996. 0100-879X 2-s2.0-0030003217 |
url |
http://hdl.handle.net/11449/224032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
629-632 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964640871972864 |