Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vetpar.2012.09.029 http://hdl.handle.net/11449/74602 |
Resumo: | In canine visceral leishmaniasis (CVL), the abnormalities most commonly observed in clinical examination on the animals are lymphadenomegaly and skin lesions. Dogs are the main domestic reservoir for the protozoon Leishmania (L.) chagasi and the skin is the main site of contamination by the vector insect. Some protozoa use apoptosis as an immunological escape mechanism. The aim of this study was to correlate the presence of apoptosis with the parasite load and with the inflammatory response in the skin and lymph nodes of dogs naturally infected with Leishmania (L.) chagasi. Thirty-three dogs from the municipality of Araçatuba (São Paulo, Brazil) were used, an endemic area for CVL. Muzzle, ear and abdominal skin and the popliteal, subscapular, iliac and mesenteric lymph nodes of symptomatic (S), oligosymptomatic (O) and asymptomatic (A) dogs were analyzed histologically. The parasite load and percentage apoptosis were evaluated using an immunohistochemical technique. Microscopically, the lymph nodes presented chronic lymphadenitis and the skin presented plasmacytic infiltrate and granulomatous foci in the superficial dermis, especially in the ear and muzzle regions. The inflammation was most severe in group S. The parasite load and apoptotic cell density were also greatest in this group. The cause of the lymphoid atrophy in these dogs was correlated with T lymphocyte apoptosis, thus leaving the dogs more susceptible to CVL. The peripheral lymph nodes presented the greatest inflammatory response. Independent of the clinical picture, the predominant inflammatory response was granulomatous and plasmacytic, both in the skin and in the peripheral lymph nodes. The ear skin presented the greatest intensity of inflammation and parasite load, followed by the muzzle skin, in group S. The ear skin area presented a non-significant difference in cell profile, with predominance of macrophages, and a significant difference from group A to groups O and S. It was seen that in these areas, there were high densities of parasites and cells undergoing apoptosis, in group S. The association between apoptosis and parasite load was not significant in the lymph nodes, but in the muzzle regions and at the ear tips, a positive correlation was seen between the parasite load and the density of cells undergoing apoptosis. The dogs in group S had the highest parasite load and the greatest number of apoptotic cells, thus suggesting that the parasite had an immune evasion mechanism, which could be proven statistically in the skin. © 2012 Elsevier B.V. |
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Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasisApoptosisDogsImmune evasionLeishmania (Leishmania) chagasiLymphadenopathySkin lesionsanimal tissueapoptosisasymptomatic infectionBrazilcontrolled studydermisfemalegranulomaimmune evasionimmunohistochemistryinflammationinflammatory infiltrateLeishmania chagasilymph nodelymphadenitismacrophagemalenonhumanparasite loadplasma cellskinT lymphocytevisceral leishmaniasisAnimaliaCanis familiarisHexapodaLeishmania donovani chagasiProtozoaIn canine visceral leishmaniasis (CVL), the abnormalities most commonly observed in clinical examination on the animals are lymphadenomegaly and skin lesions. Dogs are the main domestic reservoir for the protozoon Leishmania (L.) chagasi and the skin is the main site of contamination by the vector insect. Some protozoa use apoptosis as an immunological escape mechanism. The aim of this study was to correlate the presence of apoptosis with the parasite load and with the inflammatory response in the skin and lymph nodes of dogs naturally infected with Leishmania (L.) chagasi. Thirty-three dogs from the municipality of Araçatuba (São Paulo, Brazil) were used, an endemic area for CVL. Muzzle, ear and abdominal skin and the popliteal, subscapular, iliac and mesenteric lymph nodes of symptomatic (S), oligosymptomatic (O) and asymptomatic (A) dogs were analyzed histologically. The parasite load and percentage apoptosis were evaluated using an immunohistochemical technique. Microscopically, the lymph nodes presented chronic lymphadenitis and the skin presented plasmacytic infiltrate and granulomatous foci in the superficial dermis, especially in the ear and muzzle regions. The inflammation was most severe in group S. The parasite load and apoptotic cell density were also greatest in this group. The cause of the lymphoid atrophy in these dogs was correlated with T lymphocyte apoptosis, thus leaving the dogs more susceptible to CVL. The peripheral lymph nodes presented the greatest inflammatory response. Independent of the clinical picture, the predominant inflammatory response was granulomatous and plasmacytic, both in the skin and in the peripheral lymph nodes. The ear skin presented the greatest intensity of inflammation and parasite load, followed by the muzzle skin, in group S. The ear skin area presented a non-significant difference in cell profile, with predominance of macrophages, and a significant difference from group A to groups O and S. It was seen that in these areas, there were high densities of parasites and cells undergoing apoptosis, in group S. The association between apoptosis and parasite load was not significant in the lymph nodes, but in the muzzle regions and at the ear tips, a positive correlation was seen between the parasite load and the density of cells undergoing apoptosis. The dogs in group S had the highest parasite load and the greatest number of apoptotic cells, thus suggesting that the parasite had an immune evasion mechanism, which could be proven statistically in the skin. © 2012 Elsevier B.V.Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) UNESP, Jaboticabal, São Paulo, 14884-900Departamento de Ciências Exatas FCAV UNESP, Jaboticabal, São PauloDepartamento de Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária de Araçatuba (FMVA) UNESP, Araçatuba, São PauloDepartamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias (FCAV) UNESP, Jaboticabal, São Paulo, 14884-900Departamento de Ciências Exatas FCAV UNESP, Jaboticabal, São PauloDepartamento de Clínica Cirurgia e Reprodução Animal Faculdade de Medicina Veterinária de Araçatuba (FMVA) UNESP, Araçatuba, São PauloUniversidade Estadual Paulista (Unesp)Moreira, Pamela Rodrigues Reina [UNESP]De Barros Bandarra, Marcio [UNESP]Magalhães, Geórgia Modé [UNESP]Munari, Danísio Prado [UNESP]Machado, Gisele Fabrino [UNESP]Prandini, Marcelo Martinasso [UNESP]Alessi, Antonio Carlos [UNESP]De Oliveira Vasconcelos, Rosemeri [UNESP]2014-05-27T11:28:27Z2014-05-27T11:28:27Z2013-02-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article149-157application/pdfhttp://dx.doi.org/10.1016/j.vetpar.2012.09.029Veterinary Parasitology, v. 192, n. 1-3, p. 149-157, 2013.0304-40171873-2550http://hdl.handle.net/11449/7460210.1016/j.vetpar.2012.09.029WOS:0003140789000182-s2.0-848716984242-s2.0-84871698424.pdf0310405558125634Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Parasitology2.4221,275info:eu-repo/semantics/openAccess2023-10-05T06:03:56Zoai:repositorio.unesp.br:11449/74602Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-05T06:03:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
title |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
spellingShingle |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis Moreira, Pamela Rodrigues Reina [UNESP] Apoptosis Dogs Immune evasion Leishmania (Leishmania) chagasi Lymphadenopathy Skin lesions animal tissue apoptosis asymptomatic infection Brazil controlled study dermis female granuloma immune evasion immunohistochemistry inflammation inflammatory infiltrate Leishmania chagasi lymph node lymphadenitis macrophage male nonhuman parasite load plasma cell skin T lymphocyte visceral leishmaniasis Animalia Canis familiaris Hexapoda Leishmania donovani chagasi Protozoa |
title_short |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
title_full |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
title_fullStr |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
title_full_unstemmed |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
title_sort |
Influence of apoptosis on the cutaneous and peripheral lymph node inflammatory response in dogs with visceral leishmaniasis |
author |
Moreira, Pamela Rodrigues Reina [UNESP] |
author_facet |
Moreira, Pamela Rodrigues Reina [UNESP] De Barros Bandarra, Marcio [UNESP] Magalhães, Geórgia Modé [UNESP] Munari, Danísio Prado [UNESP] Machado, Gisele Fabrino [UNESP] Prandini, Marcelo Martinasso [UNESP] Alessi, Antonio Carlos [UNESP] De Oliveira Vasconcelos, Rosemeri [UNESP] |
author_role |
author |
author2 |
De Barros Bandarra, Marcio [UNESP] Magalhães, Geórgia Modé [UNESP] Munari, Danísio Prado [UNESP] Machado, Gisele Fabrino [UNESP] Prandini, Marcelo Martinasso [UNESP] Alessi, Antonio Carlos [UNESP] De Oliveira Vasconcelos, Rosemeri [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moreira, Pamela Rodrigues Reina [UNESP] De Barros Bandarra, Marcio [UNESP] Magalhães, Geórgia Modé [UNESP] Munari, Danísio Prado [UNESP] Machado, Gisele Fabrino [UNESP] Prandini, Marcelo Martinasso [UNESP] Alessi, Antonio Carlos [UNESP] De Oliveira Vasconcelos, Rosemeri [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Dogs Immune evasion Leishmania (Leishmania) chagasi Lymphadenopathy Skin lesions animal tissue apoptosis asymptomatic infection Brazil controlled study dermis female granuloma immune evasion immunohistochemistry inflammation inflammatory infiltrate Leishmania chagasi lymph node lymphadenitis macrophage male nonhuman parasite load plasma cell skin T lymphocyte visceral leishmaniasis Animalia Canis familiaris Hexapoda Leishmania donovani chagasi Protozoa |
topic |
Apoptosis Dogs Immune evasion Leishmania (Leishmania) chagasi Lymphadenopathy Skin lesions animal tissue apoptosis asymptomatic infection Brazil controlled study dermis female granuloma immune evasion immunohistochemistry inflammation inflammatory infiltrate Leishmania chagasi lymph node lymphadenitis macrophage male nonhuman parasite load plasma cell skin T lymphocyte visceral leishmaniasis Animalia Canis familiaris Hexapoda Leishmania donovani chagasi Protozoa |
description |
In canine visceral leishmaniasis (CVL), the abnormalities most commonly observed in clinical examination on the animals are lymphadenomegaly and skin lesions. Dogs are the main domestic reservoir for the protozoon Leishmania (L.) chagasi and the skin is the main site of contamination by the vector insect. Some protozoa use apoptosis as an immunological escape mechanism. The aim of this study was to correlate the presence of apoptosis with the parasite load and with the inflammatory response in the skin and lymph nodes of dogs naturally infected with Leishmania (L.) chagasi. Thirty-three dogs from the municipality of Araçatuba (São Paulo, Brazil) were used, an endemic area for CVL. Muzzle, ear and abdominal skin and the popliteal, subscapular, iliac and mesenteric lymph nodes of symptomatic (S), oligosymptomatic (O) and asymptomatic (A) dogs were analyzed histologically. The parasite load and percentage apoptosis were evaluated using an immunohistochemical technique. Microscopically, the lymph nodes presented chronic lymphadenitis and the skin presented plasmacytic infiltrate and granulomatous foci in the superficial dermis, especially in the ear and muzzle regions. The inflammation was most severe in group S. The parasite load and apoptotic cell density were also greatest in this group. The cause of the lymphoid atrophy in these dogs was correlated with T lymphocyte apoptosis, thus leaving the dogs more susceptible to CVL. The peripheral lymph nodes presented the greatest inflammatory response. Independent of the clinical picture, the predominant inflammatory response was granulomatous and plasmacytic, both in the skin and in the peripheral lymph nodes. The ear skin presented the greatest intensity of inflammation and parasite load, followed by the muzzle skin, in group S. The ear skin area presented a non-significant difference in cell profile, with predominance of macrophages, and a significant difference from group A to groups O and S. It was seen that in these areas, there were high densities of parasites and cells undergoing apoptosis, in group S. The association between apoptosis and parasite load was not significant in the lymph nodes, but in the muzzle regions and at the ear tips, a positive correlation was seen between the parasite load and the density of cells undergoing apoptosis. The dogs in group S had the highest parasite load and the greatest number of apoptotic cells, thus suggesting that the parasite had an immune evasion mechanism, which could be proven statistically in the skin. © 2012 Elsevier B.V. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-18 2014-05-27T11:28:27Z 2014-05-27T11:28:27Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vetpar.2012.09.029 Veterinary Parasitology, v. 192, n. 1-3, p. 149-157, 2013. 0304-4017 1873-2550 http://hdl.handle.net/11449/74602 10.1016/j.vetpar.2012.09.029 WOS:000314078900018 2-s2.0-84871698424 2-s2.0-84871698424.pdf 0310405558125634 |
url |
http://dx.doi.org/10.1016/j.vetpar.2012.09.029 http://hdl.handle.net/11449/74602 |
identifier_str_mv |
Veterinary Parasitology, v. 192, n. 1-3, p. 149-157, 2013. 0304-4017 1873-2550 10.1016/j.vetpar.2012.09.029 WOS:000314078900018 2-s2.0-84871698424 2-s2.0-84871698424.pdf 0310405558125634 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Parasitology 2.422 1,275 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
149-157 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961466427506688 |