Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1515/ap-2018-0060 http://hdl.handle.net/11449/164401 |
Resumo: | The mechanisms responsible for the imbalance between oxidants and antioxidants in sheep infected with Haemonchus contortus are not well established. This study aimed to prove the hypothesis that oxidative stress occurring during infection by H. contortus varies according to breed, and that the parasite burden correlates with hypoalbuminaemia and anaemia. Thus, after deworming and confirming the absence of infection, two different sheep breeds, Suffolk (n = 15) and Santa Ines (n = 22), were orally inoculated with a single dose of 5,000 L3 of H. contortus. The egg counts per gram of faeces (EPG), packed cell volume (PCV) and concentrations of several plasma markers of oxidative stress (lipid peroxidation, albumin, uric acid, total bilirubin, total antioxidant capacity [TAC], total oxidant concentration [TOC] and the oxidative stress index [OSI]) were quantified before (control group) and during the experimental infection (28, 34 and 42 days post-inoculation). In both breeds, TOC increased at 28 days and TAC increased at 42 days. In Suffolk sheep, there was a positive correlation of EPG with oxidant components (28 days) and a negative correlation of EPG with PCV (42 days). In Santa Ines sheep, there was a positive correlation of EPG with bilirubin (r = 0.492; p = 0.020). H. contortus infection caused oxidative stress, which varied according to the breed. Parasite burden was not associated with hypoalbuminaemia, whereas there was a negative correlation with PCV. This research provides the first evidence that the antioxidant status contributes more to the resilience to H. contortus in Santa Ines sheep compared to Suffolk sheep. |
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Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortusAntioxidant statusgastrointestinal parasiteHaemonchosislamblipid peroxidationoxidative stress indexThe mechanisms responsible for the imbalance between oxidants and antioxidants in sheep infected with Haemonchus contortus are not well established. This study aimed to prove the hypothesis that oxidative stress occurring during infection by H. contortus varies according to breed, and that the parasite burden correlates with hypoalbuminaemia and anaemia. Thus, after deworming and confirming the absence of infection, two different sheep breeds, Suffolk (n = 15) and Santa Ines (n = 22), were orally inoculated with a single dose of 5,000 L3 of H. contortus. The egg counts per gram of faeces (EPG), packed cell volume (PCV) and concentrations of several plasma markers of oxidative stress (lipid peroxidation, albumin, uric acid, total bilirubin, total antioxidant capacity [TAC], total oxidant concentration [TOC] and the oxidative stress index [OSI]) were quantified before (control group) and during the experimental infection (28, 34 and 42 days post-inoculation). In both breeds, TOC increased at 28 days and TAC increased at 42 days. In Suffolk sheep, there was a positive correlation of EPG with oxidant components (28 days) and a negative correlation of EPG with PCV (42 days). In Santa Ines sheep, there was a positive correlation of EPG with bilirubin (r = 0.492; p = 0.020). H. contortus infection caused oxidative stress, which varied according to the breed. Parasite burden was not associated with hypoalbuminaemia, whereas there was a negative correlation with PCV. This research provides the first evidence that the antioxidant status contributes more to the resilience to H. contortus in Santa Ines sheep compared to Suffolk sheep.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ, Dept Vet Med Surg & Anim Reprod, Clovis Pestana 793, BR-16050680 Aracatuba, SP, BrazilSao Paulo State Univ, Dept Support Prod & Anim Hlth, Clovis Pestana 793, BR-16050680 Aracatuba, SP, BrazilSao Paulo State Univ, Dept Vet Med Surg & Anim Reprod, Clovis Pestana 793, BR-16050680 Aracatuba, SP, BrazilSao Paulo State Univ, Dept Support Prod & Anim Hlth, Clovis Pestana 793, BR-16050680 Aracatuba, SP, BrazilWalter De Gruyter GmbhUniversidade Estadual Paulista (Unesp)Baptistiolli, Lillian [UNESP]Narciso, Luis Gustavo [UNESP]Martins de Almeida, Breno Fernando [UNESP]Bosco, Anelise Maria [UNESP]Souza, Jucilene Conceicao de [UNESP]Pintor Torrecilha, Rafaela Beatriz [UNESP]Pereira, Priscila Preve [UNESP]Figueiredo, Renata Nogueira [UNESP]Garcia, Jose Fernando [UNESP]Kaneto, Carlos Noriyuki [UNESP]Ciarlini, Paulo Cesar [UNESP]2018-11-26T17:54:24Z2018-11-26T17:54:24Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article504-514http://dx.doi.org/10.1515/ap-2018-0060Acta Parasitologica. Berlin: Walter De Gruyter Gmbh, v. 63, n. 3, p. 504-514, 2018.1230-2821http://hdl.handle.net/11449/16440110.1515/ap-2018-0060WOS:00043794610000836139400182995000000-0003-1480-5208Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Parasitologica0,641info:eu-repo/semantics/openAccess2021-10-23T19:50:08Zoai:repositorio.unesp.br:11449/164401Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:50:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
title |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
spellingShingle |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus Baptistiolli, Lillian [UNESP] Antioxidant status gastrointestinal parasite Haemonchosis lamb lipid peroxidation oxidative stress index |
title_short |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
title_full |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
title_fullStr |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
title_full_unstemmed |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
title_sort |
Systemic oxidative stress in Suffolk and Santa Ines sheep experimentally infected with Haemonchus contortus |
author |
Baptistiolli, Lillian [UNESP] |
author_facet |
Baptistiolli, Lillian [UNESP] Narciso, Luis Gustavo [UNESP] Martins de Almeida, Breno Fernando [UNESP] Bosco, Anelise Maria [UNESP] Souza, Jucilene Conceicao de [UNESP] Pintor Torrecilha, Rafaela Beatriz [UNESP] Pereira, Priscila Preve [UNESP] Figueiredo, Renata Nogueira [UNESP] Garcia, Jose Fernando [UNESP] Kaneto, Carlos Noriyuki [UNESP] Ciarlini, Paulo Cesar [UNESP] |
author_role |
author |
author2 |
Narciso, Luis Gustavo [UNESP] Martins de Almeida, Breno Fernando [UNESP] Bosco, Anelise Maria [UNESP] Souza, Jucilene Conceicao de [UNESP] Pintor Torrecilha, Rafaela Beatriz [UNESP] Pereira, Priscila Preve [UNESP] Figueiredo, Renata Nogueira [UNESP] Garcia, Jose Fernando [UNESP] Kaneto, Carlos Noriyuki [UNESP] Ciarlini, Paulo Cesar [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Baptistiolli, Lillian [UNESP] Narciso, Luis Gustavo [UNESP] Martins de Almeida, Breno Fernando [UNESP] Bosco, Anelise Maria [UNESP] Souza, Jucilene Conceicao de [UNESP] Pintor Torrecilha, Rafaela Beatriz [UNESP] Pereira, Priscila Preve [UNESP] Figueiredo, Renata Nogueira [UNESP] Garcia, Jose Fernando [UNESP] Kaneto, Carlos Noriyuki [UNESP] Ciarlini, Paulo Cesar [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant status gastrointestinal parasite Haemonchosis lamb lipid peroxidation oxidative stress index |
topic |
Antioxidant status gastrointestinal parasite Haemonchosis lamb lipid peroxidation oxidative stress index |
description |
The mechanisms responsible for the imbalance between oxidants and antioxidants in sheep infected with Haemonchus contortus are not well established. This study aimed to prove the hypothesis that oxidative stress occurring during infection by H. contortus varies according to breed, and that the parasite burden correlates with hypoalbuminaemia and anaemia. Thus, after deworming and confirming the absence of infection, two different sheep breeds, Suffolk (n = 15) and Santa Ines (n = 22), were orally inoculated with a single dose of 5,000 L3 of H. contortus. The egg counts per gram of faeces (EPG), packed cell volume (PCV) and concentrations of several plasma markers of oxidative stress (lipid peroxidation, albumin, uric acid, total bilirubin, total antioxidant capacity [TAC], total oxidant concentration [TOC] and the oxidative stress index [OSI]) were quantified before (control group) and during the experimental infection (28, 34 and 42 days post-inoculation). In both breeds, TOC increased at 28 days and TAC increased at 42 days. In Suffolk sheep, there was a positive correlation of EPG with oxidant components (28 days) and a negative correlation of EPG with PCV (42 days). In Santa Ines sheep, there was a positive correlation of EPG with bilirubin (r = 0.492; p = 0.020). H. contortus infection caused oxidative stress, which varied according to the breed. Parasite burden was not associated with hypoalbuminaemia, whereas there was a negative correlation with PCV. This research provides the first evidence that the antioxidant status contributes more to the resilience to H. contortus in Santa Ines sheep compared to Suffolk sheep. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:54:24Z 2018-11-26T17:54:24Z 2018-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1515/ap-2018-0060 Acta Parasitologica. Berlin: Walter De Gruyter Gmbh, v. 63, n. 3, p. 504-514, 2018. 1230-2821 http://hdl.handle.net/11449/164401 10.1515/ap-2018-0060 WOS:000437946100008 3613940018299500 0000-0003-1480-5208 |
url |
http://dx.doi.org/10.1515/ap-2018-0060 http://hdl.handle.net/11449/164401 |
identifier_str_mv |
Acta Parasitologica. Berlin: Walter De Gruyter Gmbh, v. 63, n. 3, p. 504-514, 2018. 1230-2821 10.1515/ap-2018-0060 WOS:000437946100008 3613940018299500 0000-0003-1480-5208 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Parasitologica 0,641 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
504-514 |
dc.publisher.none.fl_str_mv |
Walter De Gruyter Gmbh |
publisher.none.fl_str_mv |
Walter De Gruyter Gmbh |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964580827365376 |