Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10103-018-2487-8 http://hdl.handle.net/11449/176070 |
Resumo: | The objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time. The photosensitizer methylene blue at 300 uM and erythrosine at 400 uM, sensitized with low-power laser (visible red, 660 nm) and green LED (532 nm), respectively, were used for PDI. Four groups of each sample and PDI protocol were evaluated: (a) P+L+: sensitization with the photosensitizer and irradiation with light, (b) P+L−: only treatment with the photosensitizer, (c) P−L+: only irradiation with light, and (d) P−L−: without sensitization with the dye and absence of light. The results were analyzed by t test, with a significance level of 5%. The photodynamic inactivation was able to reduce the expression of all genes for both treatments, laser and LED. The fold-decrease for the genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 were 0.73, 0.39, 0.77, 0.71, 0.67, and 0.60 for laser, respectively, and 0.66, 0.61,.050, 0.43, 0.54, and 0.66 for LED, respectively. It could be concluded that PDI showed a reduction in the expression of C. albicans genes, suggesting its virulence decrease. |
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Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilmsBiofilmsCandida albicansPhotodynamic inactivationReal-time PCRVirulence factorsThe objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time. The photosensitizer methylene blue at 300 uM and erythrosine at 400 uM, sensitized with low-power laser (visible red, 660 nm) and green LED (532 nm), respectively, were used for PDI. Four groups of each sample and PDI protocol were evaluated: (a) P+L+: sensitization with the photosensitizer and irradiation with light, (b) P+L−: only treatment with the photosensitizer, (c) P−L+: only irradiation with light, and (d) P−L−: without sensitization with the dye and absence of light. The results were analyzed by t test, with a significance level of 5%. The photodynamic inactivation was able to reduce the expression of all genes for both treatments, laser and LED. The fold-decrease for the genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 were 0.73, 0.39, 0.77, 0.71, 0.67, and 0.60 for laser, respectively, and 0.66, 0.61,.050, 0.43, 0.54, and 0.66 for LED, respectively. It could be concluded that PDI showed a reduction in the expression of C. albicans genes, suggesting its virulence decrease.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, Campus of São José dos Campos, Engenheiro Francisco José Longo Avenue, 777, Jardim São DimasDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, Campus of São José dos Campos, Engenheiro Francisco José Longo Avenue, 777, Jardim São DimasFAPESP: 2013/22897-2Universidade Estadual Paulista (Unesp)Freire, Fernanda [UNESP]de Barros, Patrícia Pimentel [UNESP]Pereira, Cristiane Aparecida [UNESP]Junqueira, Juliana Campos [UNESP]Jorge, Antonio Olavo Cardoso [UNESP]2018-12-11T17:18:47Z2018-12-11T17:18:47Z2018-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1447-1454application/pdfhttp://dx.doi.org/10.1007/s10103-018-2487-8Lasers in Medical Science, v. 33, n. 7, p. 1447-1454, 2018.1435-604X0268-8921http://hdl.handle.net/11449/17607010.1007/s10103-018-2487-82-s2.0-850444466142-s2.0-85044446614.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Medical Science0,713info:eu-repo/semantics/openAccess2023-11-18T06:10:32Zoai:repositorio.unesp.br:11449/176070Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-18T06:10:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
title |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
spellingShingle |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms Freire, Fernanda [UNESP] Biofilms Candida albicans Photodynamic inactivation Real-time PCR Virulence factors |
title_short |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
title_full |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
title_fullStr |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
title_full_unstemmed |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
title_sort |
Photodynamic inactivation in the expression of the Candida albicans genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 in biofilms |
author |
Freire, Fernanda [UNESP] |
author_facet |
Freire, Fernanda [UNESP] de Barros, Patrícia Pimentel [UNESP] Pereira, Cristiane Aparecida [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author_role |
author |
author2 |
de Barros, Patrícia Pimentel [UNESP] Pereira, Cristiane Aparecida [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Freire, Fernanda [UNESP] de Barros, Patrícia Pimentel [UNESP] Pereira, Cristiane Aparecida [UNESP] Junqueira, Juliana Campos [UNESP] Jorge, Antonio Olavo Cardoso [UNESP] |
dc.subject.por.fl_str_mv |
Biofilms Candida albicans Photodynamic inactivation Real-time PCR Virulence factors |
topic |
Biofilms Candida albicans Photodynamic inactivation Real-time PCR Virulence factors |
description |
The objective of this study was to evaluate the effects of photodynamic inactivation (PDI) on Candida albicans biofilms, evaluating its effects on gene expression of ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 by yeast. Three samples of C. albicans were used in this study: a clinical sample from a patient with HIV (39S), a clinical sample from a patient with denture stomatitis lesion (Ca30), and a standard strain ATCC 18804. The quantification of gene expression was related to the production of those genes in the samples referred above using quantitative polymerase chain reaction (qPCR) assay in real time. The photosensitizer methylene blue at 300 uM and erythrosine at 400 uM, sensitized with low-power laser (visible red, 660 nm) and green LED (532 nm), respectively, were used for PDI. Four groups of each sample and PDI protocol were evaluated: (a) P+L+: sensitization with the photosensitizer and irradiation with light, (b) P+L−: only treatment with the photosensitizer, (c) P−L+: only irradiation with light, and (d) P−L−: without sensitization with the dye and absence of light. The results were analyzed by t test, with a significance level of 5%. The photodynamic inactivation was able to reduce the expression of all genes for both treatments, laser and LED. The fold-decrease for the genes ALS3, HWP1, BCR1, TEC1, CPH1, and EFG1 were 0.73, 0.39, 0.77, 0.71, 0.67, and 0.60 for laser, respectively, and 0.66, 0.61,.050, 0.43, 0.54, and 0.66 for LED, respectively. It could be concluded that PDI showed a reduction in the expression of C. albicans genes, suggesting its virulence decrease. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:47Z 2018-12-11T17:18:47Z 2018-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10103-018-2487-8 Lasers in Medical Science, v. 33, n. 7, p. 1447-1454, 2018. 1435-604X 0268-8921 http://hdl.handle.net/11449/176070 10.1007/s10103-018-2487-8 2-s2.0-85044446614 2-s2.0-85044446614.pdf |
url |
http://dx.doi.org/10.1007/s10103-018-2487-8 http://hdl.handle.net/11449/176070 |
identifier_str_mv |
Lasers in Medical Science, v. 33, n. 7, p. 1447-1454, 2018. 1435-604X 0268-8921 10.1007/s10103-018-2487-8 2-s2.0-85044446614 2-s2.0-85044446614.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Medical Science 0,713 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1447-1454 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964976480256000 |