Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0223277 http://hdl.handle.net/11449/196875 |
Resumo: | Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol' was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macro geographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale. |
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Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation SequencingLutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol' was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macro geographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Fed Mato Grosso do Sul, Programa Posgrad Doencas Infecciosas & Parasitari, Campo Grande, MS, BrazilUniv Fed Mato Grosso do Sul, Lab Parasitol Humana, Campo Grande, MS, BrazilUniv Estadual Paulista, Dept Parasitol, Lab Pesquisas & Anal Genet, Botucatu, SP, BrazilUniv Estadual Ceara, Lab Doencas Parasitarias, Fortaleza, Ceara, BrazilUniv Estadual Paulista, Dept Parasitol, Lab Pesquisas & Anal Genet, Botucatu, SP, BrazilCAPES: 001Public Library ScienceUniversidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)Univ Estadual CearaCasaril, Aline EtelvinaAlonso, Diego Peres [UNESP]Franco, Karina GarciaNiz Alvarez, Marcus Vinicius [UNESP]Gomes Barrios, Suellem PetilimFernandes, Wagner de SouzaMoura Infran, Jucelei de OliveiraMoura Rodrigues, Ana CarolineMartins Ribolla, Paulo Eduardo [UNESP]Oliveira, Alessandra Gutierrez de2020-12-10T19:58:54Z2020-12-10T19:58:54Z2019-10-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15http://dx.doi.org/10.1371/journal.pone.0223277Plos One. San Francisco: Public Library Science, v. 14, n. 10, 15 p., 2019.1932-6203http://hdl.handle.net/11449/19687510.1371/journal.pone.0223277WOS:000532398600046Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos Oneinfo:eu-repo/semantics/openAccess2021-10-23T09:41:33Zoai:repositorio.unesp.br:11449/196875Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T09:41:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
spellingShingle |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing Casaril, Aline Etelvina |
title_short |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_full |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_fullStr |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_full_unstemmed |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
title_sort |
Macrogeographic genetic structure of Lutzomyia longipalpis complex populations using Next Generation Sequencing |
author |
Casaril, Aline Etelvina |
author_facet |
Casaril, Aline Etelvina Alonso, Diego Peres [UNESP] Franco, Karina Garcia Niz Alvarez, Marcus Vinicius [UNESP] Gomes Barrios, Suellem Petilim Fernandes, Wagner de Souza Moura Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Martins Ribolla, Paulo Eduardo [UNESP] Oliveira, Alessandra Gutierrez de |
author_role |
author |
author2 |
Alonso, Diego Peres [UNESP] Franco, Karina Garcia Niz Alvarez, Marcus Vinicius [UNESP] Gomes Barrios, Suellem Petilim Fernandes, Wagner de Souza Moura Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Martins Ribolla, Paulo Eduardo [UNESP] Oliveira, Alessandra Gutierrez de |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (Unesp) Univ Estadual Ceara |
dc.contributor.author.fl_str_mv |
Casaril, Aline Etelvina Alonso, Diego Peres [UNESP] Franco, Karina Garcia Niz Alvarez, Marcus Vinicius [UNESP] Gomes Barrios, Suellem Petilim Fernandes, Wagner de Souza Moura Infran, Jucelei de Oliveira Moura Rodrigues, Ana Caroline Martins Ribolla, Paulo Eduardo [UNESP] Oliveira, Alessandra Gutierrez de |
description |
Lutzomyia longipalpis is the main vector of Leishmania infantum, the causative agent of visceral leishmaniasis in the Neotropical realm. Its taxonomic status has been widely discussed once it encompasses a complex of species. The knowledge about the genetic structure of insect vector populations helps the elucidation of components and interactions of the disease ecoepidemiology. Thus, the objective of this study was to genotypically analyze populations of the Lu. longipalpis complex from a macrogeographic perspective using Next Generation Sequencing. Polymorphism analysis of three molecular markers was used to access the levels of population genetic structure among nine different populations of sand flies. Illumina Amplicon Sequencing Protocol' was used to identify possible polymorphic sites. The library was sequenced on paired-end Illumina MiSeq platform. Significant macro geographical population differentiation was observed among Lu. longipalpis populations via PCA and DAPC analyses. Our results revealed that populations of Lu. longipalpis from the nine municipalities were grouped into three clusters. In addition, it was observed that the levels of Lu. longipalpis population structure could be associated with distance isolation. This new sequencing method allowed us to study different molecular markers after a single sequencing run, and to evaluate population and inter-species differences on a macrogeographic scale. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-03 2020-12-10T19:58:54Z 2020-12-10T19:58:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0223277 Plos One. San Francisco: Public Library Science, v. 14, n. 10, 15 p., 2019. 1932-6203 http://hdl.handle.net/11449/196875 10.1371/journal.pone.0223277 WOS:000532398600046 |
url |
http://dx.doi.org/10.1371/journal.pone.0223277 http://hdl.handle.net/11449/196875 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 14, n. 10, 15 p., 2019. 1932-6203 10.1371/journal.pone.0223277 WOS:000532398600046 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15 |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964503155146752 |