Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10856-019-6348-1 http://hdl.handle.net/11449/199853 |
Resumo: | Calcium aluminate cement (CAC) as a biomaterial has been evaluated for its physical, mechanical and biocompatibility properties. Furthermore, the application of CAC for bone repair is due to its composition and coefficient of thermal expansion, which is similar to that of human bone. Thus, the aim of this study was to evaluate compositions of CAC-based blends as substitutes for bone defects. Five compositions of blends (alumina, zirconia, hydroxyapatite, tricalcium phosphate, chitosan), in addition to the base cement consisting of homogeneous CAC were evaluated as a substitute for bone repair. Additionally, the monotypic biofilm formation was assessed. Creation of a monocortical bone defect was performed on the femurs of rats, which were randomly filled with the different materials. The polymethylmethacrylate (PMMA) group was used as a control. All the animals were euthanized 04 weeks after the surgery procedure. Subsequently, computerized microtomography, histological and histomorphometric analyses were performed to verify the bone repair. To evaluate the formation of biofilms, reference strains of Staphylococcus aureus, Streptococcus mutans and Pseudomonas aeruginosa were cultured on the samples, and the biofilm formed was quantified by the MTT method. In the microtomography and histomorphometry results, it was observed that the blends exhibited better results than the control group, with statistically significant differences (p < 0.05) for alumina and zirconia blends. In the biofilm formation, a statistical difference (p < 0.05) in general was observed between the alumina blends and the control group (p < 0.05). It was concluded that CAC-based blends with alumina and zirconia are promising for use in fillings for bone repair. [Figure not available: see fulltext.]. |
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Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defectsCalcium aluminate cement (CAC) as a biomaterial has been evaluated for its physical, mechanical and biocompatibility properties. Furthermore, the application of CAC for bone repair is due to its composition and coefficient of thermal expansion, which is similar to that of human bone. Thus, the aim of this study was to evaluate compositions of CAC-based blends as substitutes for bone defects. Five compositions of blends (alumina, zirconia, hydroxyapatite, tricalcium phosphate, chitosan), in addition to the base cement consisting of homogeneous CAC were evaluated as a substitute for bone repair. Additionally, the monotypic biofilm formation was assessed. Creation of a monocortical bone defect was performed on the femurs of rats, which were randomly filled with the different materials. The polymethylmethacrylate (PMMA) group was used as a control. All the animals were euthanized 04 weeks after the surgery procedure. Subsequently, computerized microtomography, histological and histomorphometric analyses were performed to verify the bone repair. To evaluate the formation of biofilms, reference strains of Staphylococcus aureus, Streptococcus mutans and Pseudomonas aeruginosa were cultured on the samples, and the biofilm formed was quantified by the MTT method. In the microtomography and histomorphometry results, it was observed that the blends exhibited better results than the control group, with statistically significant differences (p < 0.05) for alumina and zirconia blends. In the biofilm formation, a statistical difference (p < 0.05) in general was observed between the alumina blends and the control group (p < 0.05). It was concluded that CAC-based blends with alumina and zirconia are promising for use in fillings for bone repair. [Figure not available: see fulltext.].Department of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos University Estadual Paulista-UNESPInstitute for Research and Development University of Vale do ParaíbaGraduated student of Institute of Science and Technology of São José dos Campos University Estadual Paulista-UNESPDepartment of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos University Estadual Paulista-UNESPGraduated student of Institute of Science and Technology of São José dos Campos University Estadual Paulista-UNESPUniversidade Estadual Paulista (Unesp)University of Vale do Paraíbade Vasconcellos, Luana Marotta Reis [UNESP]Camporês, Kaíke Lessa [UNESP]de Alcântara Abdala, Julia MarinzeckVieira, Marilia Nanni [UNESP]de Oliveira, Ivone Regina2020-12-12T01:51:04Z2020-12-12T01:51:04Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10856-019-6348-1Journal of Materials Science: Materials in Medicine, v. 31, n. 1, 2020.1573-48380957-4530http://hdl.handle.net/11449/19985310.1007/s10856-019-6348-12-s2.0-85076933578Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Science: Materials in Medicineinfo:eu-repo/semantics/openAccess2021-10-23T10:11:05Zoai:repositorio.unesp.br:11449/199853Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:11:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
title |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
spellingShingle |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects de Vasconcellos, Luana Marotta Reis [UNESP] |
title_short |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
title_full |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
title_fullStr |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
title_full_unstemmed |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
title_sort |
Biological and microbiological behavior of calcium aluminate cement-based blend for filling of bone defects |
author |
de Vasconcellos, Luana Marotta Reis [UNESP] |
author_facet |
de Vasconcellos, Luana Marotta Reis [UNESP] Camporês, Kaíke Lessa [UNESP] de Alcântara Abdala, Julia Marinzeck Vieira, Marilia Nanni [UNESP] de Oliveira, Ivone Regina |
author_role |
author |
author2 |
Camporês, Kaíke Lessa [UNESP] de Alcântara Abdala, Julia Marinzeck Vieira, Marilia Nanni [UNESP] de Oliveira, Ivone Regina |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Vale do Paraíba |
dc.contributor.author.fl_str_mv |
de Vasconcellos, Luana Marotta Reis [UNESP] Camporês, Kaíke Lessa [UNESP] de Alcântara Abdala, Julia Marinzeck Vieira, Marilia Nanni [UNESP] de Oliveira, Ivone Regina |
description |
Calcium aluminate cement (CAC) as a biomaterial has been evaluated for its physical, mechanical and biocompatibility properties. Furthermore, the application of CAC for bone repair is due to its composition and coefficient of thermal expansion, which is similar to that of human bone. Thus, the aim of this study was to evaluate compositions of CAC-based blends as substitutes for bone defects. Five compositions of blends (alumina, zirconia, hydroxyapatite, tricalcium phosphate, chitosan), in addition to the base cement consisting of homogeneous CAC were evaluated as a substitute for bone repair. Additionally, the monotypic biofilm formation was assessed. Creation of a monocortical bone defect was performed on the femurs of rats, which were randomly filled with the different materials. The polymethylmethacrylate (PMMA) group was used as a control. All the animals were euthanized 04 weeks after the surgery procedure. Subsequently, computerized microtomography, histological and histomorphometric analyses were performed to verify the bone repair. To evaluate the formation of biofilms, reference strains of Staphylococcus aureus, Streptococcus mutans and Pseudomonas aeruginosa were cultured on the samples, and the biofilm formed was quantified by the MTT method. In the microtomography and histomorphometry results, it was observed that the blends exhibited better results than the control group, with statistically significant differences (p < 0.05) for alumina and zirconia blends. In the biofilm formation, a statistical difference (p < 0.05) in general was observed between the alumina blends and the control group (p < 0.05). It was concluded that CAC-based blends with alumina and zirconia are promising for use in fillings for bone repair. [Figure not available: see fulltext.]. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:51:04Z 2020-12-12T01:51:04Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10856-019-6348-1 Journal of Materials Science: Materials in Medicine, v. 31, n. 1, 2020. 1573-4838 0957-4530 http://hdl.handle.net/11449/199853 10.1007/s10856-019-6348-1 2-s2.0-85076933578 |
url |
http://dx.doi.org/10.1007/s10856-019-6348-1 http://hdl.handle.net/11449/199853 |
identifier_str_mv |
Journal of Materials Science: Materials in Medicine, v. 31, n. 1, 2020. 1573-4838 0957-4530 10.1007/s10856-019-6348-1 2-s2.0-85076933578 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Materials Science: Materials in Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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