Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2018.01.144 http://hdl.handle.net/11449/175814 |
Resumo: | Among the bioremediation processes, biostimulation is an effective methodology for the decontamination of organic waste by the addition of agents that stimulate the indigenous microbiota development. Rice hull is a biostimulating agent that promotes the aeration of edaphic systems and stimulates the aerobiotic activity of soil microorganisms. The present study aimed to evaluate the efficacy of the bioremediation and biostimulation processes in reducing the toxicity of sewage sludge (SS) and to evaluate its possible application in agriculture using cytotoxic and genotoxic assays in human hepatoma cells (HepG2). SS of domestic origin was tested as both the pure product (PSS) and mixed with soil (S) and with a stimulating agent, such as rice hull (RH), in different proportions (SS + S and SS + S + RH); we also examined different remediation periods (3 months - T1 and 6 months - T2). For the PSS sample, a significant induction of micronucleus (MN) in T2 was observed with nuclear buds in all of the periods assessed, and we observed the presence of more than one alteration per cell (MN and nuclear bud) in T1 and T2. The PSS sample caused genotoxic effects in the HepG2 cells even after being bioremediated. For the samples containing soil and/or rice hull, no toxic effects were observed in the test system used. Therefore, the addition of SS to agricultural soils should be conducted with caution, and it is important that the SS undergoes a remediation process, such as bioremediation and biostimulation treatments. |
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Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assaysBioremediationGenotoxicityHepG2MicronucleusRice hullAmong the bioremediation processes, biostimulation is an effective methodology for the decontamination of organic waste by the addition of agents that stimulate the indigenous microbiota development. Rice hull is a biostimulating agent that promotes the aeration of edaphic systems and stimulates the aerobiotic activity of soil microorganisms. The present study aimed to evaluate the efficacy of the bioremediation and biostimulation processes in reducing the toxicity of sewage sludge (SS) and to evaluate its possible application in agriculture using cytotoxic and genotoxic assays in human hepatoma cells (HepG2). SS of domestic origin was tested as both the pure product (PSS) and mixed with soil (S) and with a stimulating agent, such as rice hull (RH), in different proportions (SS + S and SS + S + RH); we also examined different remediation periods (3 months - T1 and 6 months - T2). For the PSS sample, a significant induction of micronucleus (MN) in T2 was observed with nuclear buds in all of the periods assessed, and we observed the presence of more than one alteration per cell (MN and nuclear bud) in T1 and T2. The PSS sample caused genotoxic effects in the HepG2 cells even after being bioremediated. For the samples containing soil and/or rice hull, no toxic effects were observed in the test system used. Therefore, the addition of SS to agricultural soils should be conducted with caution, and it is important that the SS undergoes a remediation process, such as bioremediation and biostimulation treatments.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Biology Institute of Biosciences São Paulo State University (Unesp), Av. 24-A, 1515Department of Analytical Chemistry Institute of Chemistry São Paulo State University (Unesp), Rua Professor Francisco Degni, 55Department of Biology Institute of Biosciences São Paulo State University (Unesp), Av. 24-A, 1515Department of Analytical Chemistry Institute of Chemistry São Paulo State University (Unesp), Rua Professor Francisco Degni, 55Universidade Estadual Paulista (Unesp)Sommaggio, Lais Roberta Deroldo [UNESP]Mazzeo, Dânia Elisa Christofoletti [UNESP]Pamplona-Silva, Maria Tereza [UNESP]Marin-Morales, Maria Aparecida [UNESP]2018-12-11T17:17:41Z2018-12-11T17:17:41Z2018-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10-15application/pdfhttp://dx.doi.org/10.1016/j.chemosphere.2018.01.144Chemosphere, v. 199, p. 10-15.1879-12980045-6535http://hdl.handle.net/11449/17581410.1016/j.chemosphere.2018.01.1442-s2.0-850413838632-s2.0-85041383863.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphere1,435info:eu-repo/semantics/openAccess2023-10-01T06:08:58Zoai:repositorio.unesp.br:11449/175814Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-01T06:08:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
title |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
spellingShingle |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays Sommaggio, Lais Roberta Deroldo [UNESP] Bioremediation Genotoxicity HepG2 Micronucleus Rice hull |
title_short |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
title_full |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
title_fullStr |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
title_full_unstemmed |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
title_sort |
Evaluation of the potential agricultural use of biostimulated sewage sludge using mammalian cell culture assays |
author |
Sommaggio, Lais Roberta Deroldo [UNESP] |
author_facet |
Sommaggio, Lais Roberta Deroldo [UNESP] Mazzeo, Dânia Elisa Christofoletti [UNESP] Pamplona-Silva, Maria Tereza [UNESP] Marin-Morales, Maria Aparecida [UNESP] |
author_role |
author |
author2 |
Mazzeo, Dânia Elisa Christofoletti [UNESP] Pamplona-Silva, Maria Tereza [UNESP] Marin-Morales, Maria Aparecida [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sommaggio, Lais Roberta Deroldo [UNESP] Mazzeo, Dânia Elisa Christofoletti [UNESP] Pamplona-Silva, Maria Tereza [UNESP] Marin-Morales, Maria Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Bioremediation Genotoxicity HepG2 Micronucleus Rice hull |
topic |
Bioremediation Genotoxicity HepG2 Micronucleus Rice hull |
description |
Among the bioremediation processes, biostimulation is an effective methodology for the decontamination of organic waste by the addition of agents that stimulate the indigenous microbiota development. Rice hull is a biostimulating agent that promotes the aeration of edaphic systems and stimulates the aerobiotic activity of soil microorganisms. The present study aimed to evaluate the efficacy of the bioremediation and biostimulation processes in reducing the toxicity of sewage sludge (SS) and to evaluate its possible application in agriculture using cytotoxic and genotoxic assays in human hepatoma cells (HepG2). SS of domestic origin was tested as both the pure product (PSS) and mixed with soil (S) and with a stimulating agent, such as rice hull (RH), in different proportions (SS + S and SS + S + RH); we also examined different remediation periods (3 months - T1 and 6 months - T2). For the PSS sample, a significant induction of micronucleus (MN) in T2 was observed with nuclear buds in all of the periods assessed, and we observed the presence of more than one alteration per cell (MN and nuclear bud) in T1 and T2. The PSS sample caused genotoxic effects in the HepG2 cells even after being bioremediated. For the samples containing soil and/or rice hull, no toxic effects were observed in the test system used. Therefore, the addition of SS to agricultural soils should be conducted with caution, and it is important that the SS undergoes a remediation process, such as bioremediation and biostimulation treatments. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:41Z 2018-12-11T17:17:41Z 2018-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2018.01.144 Chemosphere, v. 199, p. 10-15. 1879-1298 0045-6535 http://hdl.handle.net/11449/175814 10.1016/j.chemosphere.2018.01.144 2-s2.0-85041383863 2-s2.0-85041383863.pdf |
url |
http://dx.doi.org/10.1016/j.chemosphere.2018.01.144 http://hdl.handle.net/11449/175814 |
identifier_str_mv |
Chemosphere, v. 199, p. 10-15. 1879-1298 0045-6535 10.1016/j.chemosphere.2018.01.144 2-s2.0-85041383863 2-s2.0-85041383863.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere 1,435 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10-15 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961434980712448 |