Analysis of cancer stem cells in dog's mammary neoplasias
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/227578 |
Resumo: | Mammary tumors are the most frequent cancers in dogs, representing about 50% of tumors, and have a higher incidence in females of middle aged and elderly. These tumors have been used as a model for breast cancer in women due to several common characteristics such as histological and immunohistochemical similarities. In the last decade, studies based on molecular profiles of breast cancer, made possible the identification of some neoplastic cells with characteristics of stem cells - cancer stem cells (CSC). One of the putative molecules of CSCs is CD44. Recent studies have established a crucial link between the epithelial-mesenchymal transition (EMT) and the acquisition of molecular and functional properties of stem cells. For that reason we analyzed the expression of proteins CD44, Cytokeratins AE1/AE3 and Vimentin, in dogs mammary tumors, to investigate the potencial for CSC markers, and its relation with the EMT using immunohistochemistry in paraffin embedded tissues making use of techniques such as Tissue MicroArrays (TMA). Immunostaining of cytokeratin had no significant difference between benign and malignant tumors (p ≥ 0,05), being more intense in malignant tumors. However vimentina showed higher staining intensity in benign tumors, but with no significant difference (p ≤ 0,05). The expression of CD44 was higher in malignant tumors that have greater proliferative and metastatic potencial, however its relation with EMT was not detected in the analyzed tumors. The techniques applied for the TMAs were efficient and can be used in routine and later researches. |
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Analysis of cancer stem cells in dog's mammary neoplasiasAnálise das células tronco tumorais em neoplasias mamárias de cãesCancer stem cellsCD44DogImmunohistochemistryMammary tumors are the most frequent cancers in dogs, representing about 50% of tumors, and have a higher incidence in females of middle aged and elderly. These tumors have been used as a model for breast cancer in women due to several common characteristics such as histological and immunohistochemical similarities. In the last decade, studies based on molecular profiles of breast cancer, made possible the identification of some neoplastic cells with characteristics of stem cells - cancer stem cells (CSC). One of the putative molecules of CSCs is CD44. Recent studies have established a crucial link between the epithelial-mesenchymal transition (EMT) and the acquisition of molecular and functional properties of stem cells. For that reason we analyzed the expression of proteins CD44, Cytokeratins AE1/AE3 and Vimentin, in dogs mammary tumors, to investigate the potencial for CSC markers, and its relation with the EMT using immunohistochemistry in paraffin embedded tissues making use of techniques such as Tissue MicroArrays (TMA). Immunostaining of cytokeratin had no significant difference between benign and malignant tumors (p ≥ 0,05), being more intense in malignant tumors. However vimentina showed higher staining intensity in benign tumors, but with no significant difference (p ≤ 0,05). The expression of CD44 was higher in malignant tumors that have greater proliferative and metastatic potencial, however its relation with EMT was not detected in the analyzed tumors. The techniques applied for the TMAs were efficient and can be used in routine and later researches.Programa de Pós-Graduação em Ciência Animal Universidade Paulista 'Júlio de Mesquita Filho' (Unesp-Araçatuba), Rua Clovis Pestana, 793, Jardim Dona Amelia, Araçatuba, SP 16050-680Departamento de Apoio Produção e Saúde Animal UNESP-Araçatuba, Rua Clovis Pestana, 793, Jardim Dona Amelia, Araçatuba, SP 16050-680Departamento de Clínica Cirurgia e Reprodução Animal UNESP-Araçatuba, Rua Clovis Pestana, 793, Jardim Dona Amelia, Araçatuba, SP 16050-680Programa de Pós-Graduação em Ciência Animal Universidade Paulista 'Júlio de Mesquita Filho' (Unesp-Araçatuba), Rua Clovis Pestana, 793, Jardim Dona Amelia, Araçatuba, SP 16050-680Departamento de Apoio Produção e Saúde Animal UNESP-Araçatuba, Rua Clovis Pestana, 793, Jardim Dona Amelia, Araçatuba, SP 16050-680Departamento de Clínica Cirurgia e Reprodução Animal UNESP-Araçatuba, Rua Clovis Pestana, 793, Jardim Dona Amelia, Araçatuba, SP 16050-680Universidade Estadual Paulista (UNESP)Gouveia, Gabriela Mayumi [UNESP]Paiva, Milla Bezerra [UNESP]Perri, Silvia Helena Venturoli [UNESP]Luvizotto, Maria Cecília Rui [UNESP]2022-04-29T07:13:57Z2022-04-29T07:13:57Z2013-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article229-235Revista Brasileira de Medicina Veterinaria, v. 35, n. 3, p. 229-235, 2013.2527-21790100-2430http://hdl.handle.net/11449/2275782-s2.0-84894341867Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Medicina Veterinariainfo:eu-repo/semantics/openAccess2022-04-29T07:13:57Zoai:repositorio.unesp.br:11449/227578Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:13:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of cancer stem cells in dog's mammary neoplasias Análise das células tronco tumorais em neoplasias mamárias de cães |
title |
Analysis of cancer stem cells in dog's mammary neoplasias |
spellingShingle |
Analysis of cancer stem cells in dog's mammary neoplasias Gouveia, Gabriela Mayumi [UNESP] Cancer stem cells CD44 Dog Immunohistochemistry |
title_short |
Analysis of cancer stem cells in dog's mammary neoplasias |
title_full |
Analysis of cancer stem cells in dog's mammary neoplasias |
title_fullStr |
Analysis of cancer stem cells in dog's mammary neoplasias |
title_full_unstemmed |
Analysis of cancer stem cells in dog's mammary neoplasias |
title_sort |
Analysis of cancer stem cells in dog's mammary neoplasias |
author |
Gouveia, Gabriela Mayumi [UNESP] |
author_facet |
Gouveia, Gabriela Mayumi [UNESP] Paiva, Milla Bezerra [UNESP] Perri, Silvia Helena Venturoli [UNESP] Luvizotto, Maria Cecília Rui [UNESP] |
author_role |
author |
author2 |
Paiva, Milla Bezerra [UNESP] Perri, Silvia Helena Venturoli [UNESP] Luvizotto, Maria Cecília Rui [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Gouveia, Gabriela Mayumi [UNESP] Paiva, Milla Bezerra [UNESP] Perri, Silvia Helena Venturoli [UNESP] Luvizotto, Maria Cecília Rui [UNESP] |
dc.subject.por.fl_str_mv |
Cancer stem cells CD44 Dog Immunohistochemistry |
topic |
Cancer stem cells CD44 Dog Immunohistochemistry |
description |
Mammary tumors are the most frequent cancers in dogs, representing about 50% of tumors, and have a higher incidence in females of middle aged and elderly. These tumors have been used as a model for breast cancer in women due to several common characteristics such as histological and immunohistochemical similarities. In the last decade, studies based on molecular profiles of breast cancer, made possible the identification of some neoplastic cells with characteristics of stem cells - cancer stem cells (CSC). One of the putative molecules of CSCs is CD44. Recent studies have established a crucial link between the epithelial-mesenchymal transition (EMT) and the acquisition of molecular and functional properties of stem cells. For that reason we analyzed the expression of proteins CD44, Cytokeratins AE1/AE3 and Vimentin, in dogs mammary tumors, to investigate the potencial for CSC markers, and its relation with the EMT using immunohistochemistry in paraffin embedded tissues making use of techniques such as Tissue MicroArrays (TMA). Immunostaining of cytokeratin had no significant difference between benign and malignant tumors (p ≥ 0,05), being more intense in malignant tumors. However vimentina showed higher staining intensity in benign tumors, but with no significant difference (p ≤ 0,05). The expression of CD44 was higher in malignant tumors that have greater proliferative and metastatic potencial, however its relation with EMT was not detected in the analyzed tumors. The techniques applied for the TMAs were efficient and can be used in routine and later researches. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 2022-04-29T07:13:57Z 2022-04-29T07:13:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Brasileira de Medicina Veterinaria, v. 35, n. 3, p. 229-235, 2013. 2527-2179 0100-2430 http://hdl.handle.net/11449/227578 2-s2.0-84894341867 |
identifier_str_mv |
Revista Brasileira de Medicina Veterinaria, v. 35, n. 3, p. 229-235, 2013. 2527-2179 0100-2430 2-s2.0-84894341867 |
url |
http://hdl.handle.net/11449/227578 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Medicina Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
229-235 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965400287412224 |