First successful frozen semen of the maned wolf (Chrysocyon brachyurus)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1111/rda.13999 http://hdl.handle.net/11449/229375 |
Resumo: | This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris–yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris–yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs. |
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First successful frozen semen of the maned wolf (Chrysocyon brachyurus)captive-wolfcryopreservationspermatozoaThis study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris–yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris–yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs.Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)University of Franca (UNIFRAN)Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)University of Franca (UNIFRAN)Teodoro, Laura O. [UNESP]Camargo, Laiza S. [UNESP]Scheeren, Verônica F. C. [UNESP]Freitas-Dell'Aqua, Camila P. [UNESP]Papa, Frederico O. [UNESP]Honsho, Cristiane S.Souza, Fabiana F. [UNESP]2022-04-29T08:32:10Z2022-04-29T08:32:10Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/rda.13999Reproduction in Domestic Animals.1439-05310936-6768http://hdl.handle.net/11449/22937510.1111/rda.139992-s2.0-85113169581Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccess2022-04-29T08:32:10Zoai:repositorio.unesp.br:11449/229375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:32:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| title |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| spellingShingle |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) Teodoro, Laura O. [UNESP] captive-wolf cryopreservation spermatozoa |
| title_short |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| title_full |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| title_fullStr |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| title_full_unstemmed |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| title_sort |
First successful frozen semen of the maned wolf (Chrysocyon brachyurus) |
| author |
Teodoro, Laura O. [UNESP] |
| author_facet |
Teodoro, Laura O. [UNESP] Camargo, Laiza S. [UNESP] Scheeren, Verônica F. C. [UNESP] Freitas-Dell'Aqua, Camila P. [UNESP] Papa, Frederico O. [UNESP] Honsho, Cristiane S. Souza, Fabiana F. [UNESP] |
| author_role |
author |
| author2 |
Camargo, Laiza S. [UNESP] Scheeren, Verônica F. C. [UNESP] Freitas-Dell'Aqua, Camila P. [UNESP] Papa, Frederico O. [UNESP] Honsho, Cristiane S. Souza, Fabiana F. [UNESP] |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Franca (UNIFRAN) |
| dc.contributor.author.fl_str_mv |
Teodoro, Laura O. [UNESP] Camargo, Laiza S. [UNESP] Scheeren, Verônica F. C. [UNESP] Freitas-Dell'Aqua, Camila P. [UNESP] Papa, Frederico O. [UNESP] Honsho, Cristiane S. Souza, Fabiana F. [UNESP] |
| dc.subject.por.fl_str_mv |
captive-wolf cryopreservation spermatozoa |
| topic |
captive-wolf cryopreservation spermatozoa |
| description |
This study aimed to describe successful cryopreservation of sperm from maned wolves (Chrysocyon brachyurus). Three ejaculates from 2 maned wolves were collected by digital manipulation of the penis and evaluated subjectively, centrifuged and frozen in BotuCrio® (Botupharma, Botucatu, Brazil) or Tris–yolk egg extender. Spermatozoa were thawed at 37ºC/30s or 70ºC/4s and evaluated for kinetics, morphology, plasma and acrosome membrane integrity, mitochondrial potential, hydrogen peroxide, superoxide anion and lipid peroxidation. From 5 thawed samples, two had sperm total motility >55% (56.0% and 64.0%) and progressive motility ~35% (35% and 40%), both frozen with Tris–yolk egg. Plasma and acrosome membrane integrity decreased and percentage of sperm defects increased post-thawing. We concluded that is possible to freeze spermatozoa from maned wolves using semen collection and processing methods applied for domestic dogs. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:32:10Z 2022-04-29T08:32:10Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/rda.13999 Reproduction in Domestic Animals. 1439-0531 0936-6768 http://hdl.handle.net/11449/229375 10.1111/rda.13999 2-s2.0-85113169581 |
| url |
http://dx.doi.org/10.1111/rda.13999 http://hdl.handle.net/11449/229375 |
| identifier_str_mv |
Reproduction in Domestic Animals. 1439-0531 0936-6768 10.1111/rda.13999 2-s2.0-85113169581 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Reproduction in Domestic Animals |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
| reponame_str |
Repositório Institucional da UNESP |
| collection |
Repositório Institucional da UNESP |
| repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
| repository.mail.fl_str_mv |
|
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1797789987127689216 |