Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.21577/0100-4042.20170885 http://hdl.handle.net/11449/237710 |
Resumo: | The genus Chamaecrista comprises more than 330 species, with only a few studies on their chemical composition and biologic activities. In this study, the phytochemical profile of leaf, stems, and fruits extracts of the C. duckeana were examined by UPLC-ESI-HRMS analysis to determine possibly bioactive constituents. The antioxidant activity was carried out through in vitro assay, by the sequestration of the free radical DPPH. To evaluate the cytotoxic activity of the extracts, an MTT assay was used and the IC50 was determined against HL60 and RAJI cell lines. The metabolic profiles of the botanical parts are dominated by flavonoid class, highlighting isoflavonoids such as daidzin and ononin. All these compounds are reported for the first time in C. duckeana. The extracts presented antioxidant potential, and the activity of the stems extract was higher than the standard butylated hydroxytoluene. In the cytotoxic assay, only HL60 line (leukemia) had growth inhibition over 80%. The stems presented more expressive cytotoxicity with IC50 of 137.3 (104.6-180.1) and 106.8 (96.52-118.3) mu mol. L-1 for HL60 and RAJI, respectively. In conclusion, the present work provides an in-depth knowledge about the chemical profile of C. duckeana, a species rich in bioactive secondary metabolites with cytotoxic activity. |
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Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE)Chamaecrista duckeanaUPLC-ESI-HRMSCytotoxicityIsoflavonoidsThe genus Chamaecrista comprises more than 330 species, with only a few studies on their chemical composition and biologic activities. In this study, the phytochemical profile of leaf, stems, and fruits extracts of the C. duckeana were examined by UPLC-ESI-HRMS analysis to determine possibly bioactive constituents. The antioxidant activity was carried out through in vitro assay, by the sequestration of the free radical DPPH. To evaluate the cytotoxic activity of the extracts, an MTT assay was used and the IC50 was determined against HL60 and RAJI cell lines. The metabolic profiles of the botanical parts are dominated by flavonoid class, highlighting isoflavonoids such as daidzin and ononin. All these compounds are reported for the first time in C. duckeana. The extracts presented antioxidant potential, and the activity of the stems extract was higher than the standard butylated hydroxytoluene. In the cytotoxic assay, only HL60 line (leukemia) had growth inhibition over 80%. The stems presented more expressive cytotoxicity with IC50 of 137.3 (104.6-180.1) and 106.8 (96.52-118.3) mu mol. L-1 for HL60 and RAJI, respectively. In conclusion, the present work provides an in-depth knowledge about the chemical profile of C. duckeana, a species rich in bioactive secondary metabolites with cytotoxic activity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Fed Ceara, Dept Quim Anal & Fisicoquim, BR-60455970 Fortaleza, Ceara, BrazilUniv Fed Ceara, Dept Fisiol & Farmacol, BR-60431970 Fortaleza, Ceara, BrazilUniv Estadual Paulista, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilUniv Estadual Paulista, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilCNPq: 465637/2014-0/FAPESP 2014/50926-0FAPESP: 2013/07600-3Soc Brasileira QuimicaUniv Fed CearaUniversidade Estadual Paulista (UNESP)Lima, Daniele Rodrigues deFranca, Maria Gleiziane de AraujoOliveira, Fatima de Cassia Evangelista dePessoa, Claudia do O.Cavalheiro, Alberto Jose [UNESP]Silva, Maria Goretti de Vasconcelos2022-11-30T13:42:37Z2022-11-30T13:42:37Z2022-04-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4http://dx.doi.org/10.21577/0100-4042.20170885Quimica Nova. Sao Paulo: Soc Brasileira Quimica, 4 p., 2022.0100-4042http://hdl.handle.net/11449/23771010.21577/0100-4042.20170885WOS:000783148100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengQuimica Novainfo:eu-repo/semantics/openAccess2022-11-30T13:42:37Zoai:repositorio.unesp.br:11449/237710Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-11-30T13:42:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
title |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
spellingShingle |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) Lima, Daniele Rodrigues de Chamaecrista duckeana UPLC-ESI-HRMS Cytotoxicity Isoflavonoids |
title_short |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
title_full |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
title_fullStr |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
title_full_unstemmed |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
title_sort |
Metabolic profiling and cytotoxic activity of methanol extracts from Chamaecrista duckeana (P. BEZERRA & A. FERN.) H. S. IRWIN & BARNEBY (LEGUMINOSAE, CAESALPINIOIDEAE) |
author |
Lima, Daniele Rodrigues de |
author_facet |
Lima, Daniele Rodrigues de Franca, Maria Gleiziane de Araujo Oliveira, Fatima de Cassia Evangelista de Pessoa, Claudia do O. Cavalheiro, Alberto Jose [UNESP] Silva, Maria Goretti de Vasconcelos |
author_role |
author |
author2 |
Franca, Maria Gleiziane de Araujo Oliveira, Fatima de Cassia Evangelista de Pessoa, Claudia do O. Cavalheiro, Alberto Jose [UNESP] Silva, Maria Goretti de Vasconcelos |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Univ Fed Ceara Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Lima, Daniele Rodrigues de Franca, Maria Gleiziane de Araujo Oliveira, Fatima de Cassia Evangelista de Pessoa, Claudia do O. Cavalheiro, Alberto Jose [UNESP] Silva, Maria Goretti de Vasconcelos |
dc.subject.por.fl_str_mv |
Chamaecrista duckeana UPLC-ESI-HRMS Cytotoxicity Isoflavonoids |
topic |
Chamaecrista duckeana UPLC-ESI-HRMS Cytotoxicity Isoflavonoids |
description |
The genus Chamaecrista comprises more than 330 species, with only a few studies on their chemical composition and biologic activities. In this study, the phytochemical profile of leaf, stems, and fruits extracts of the C. duckeana were examined by UPLC-ESI-HRMS analysis to determine possibly bioactive constituents. The antioxidant activity was carried out through in vitro assay, by the sequestration of the free radical DPPH. To evaluate the cytotoxic activity of the extracts, an MTT assay was used and the IC50 was determined against HL60 and RAJI cell lines. The metabolic profiles of the botanical parts are dominated by flavonoid class, highlighting isoflavonoids such as daidzin and ononin. All these compounds are reported for the first time in C. duckeana. The extracts presented antioxidant potential, and the activity of the stems extract was higher than the standard butylated hydroxytoluene. In the cytotoxic assay, only HL60 line (leukemia) had growth inhibition over 80%. The stems presented more expressive cytotoxicity with IC50 of 137.3 (104.6-180.1) and 106.8 (96.52-118.3) mu mol. L-1 for HL60 and RAJI, respectively. In conclusion, the present work provides an in-depth knowledge about the chemical profile of C. duckeana, a species rich in bioactive secondary metabolites with cytotoxic activity. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:42:37Z 2022-11-30T13:42:37Z 2022-04-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.21577/0100-4042.20170885 Quimica Nova. Sao Paulo: Soc Brasileira Quimica, 4 p., 2022. 0100-4042 http://hdl.handle.net/11449/237710 10.21577/0100-4042.20170885 WOS:000783148100001 |
url |
http://dx.doi.org/10.21577/0100-4042.20170885 http://hdl.handle.net/11449/237710 |
identifier_str_mv |
Quimica Nova. Sao Paulo: Soc Brasileira Quimica, 4 p., 2022. 0100-4042 10.21577/0100-4042.20170885 WOS:000783148100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Quimica Nova |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4 |
dc.publisher.none.fl_str_mv |
Soc Brasileira Quimica |
publisher.none.fl_str_mv |
Soc Brasileira Quimica |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964790705094656 |