Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.transproceed.2018.07.021 http://hdl.handle.net/11449/185280 |
Resumo: | Background. Ischemia-reperfusion injury is an unavoidable aspect of transplantation, as well as an important cause of acute kidney injury in clinical practice. Pre- and post-ischemic conditioning are strategies that may provide organs with resistance to major ischemic events. This study evaluates the effects of ischemic preconditioning and ischemic postconditioning, either separately or in combination, after an acute ischemia-reperfusion kidney injury. Methods. Forty Wistar rats received isoflurane anesthesia and were randomized into 5 groups: 1. the sham group underwent laparotomy; 2. the control group underwent laparotomy and 30 minutes of renal ischemia followed by reperfusion; 3. the preconditioning group underwent laparotomy, ischemic preconditioning, and 30 minutes of renal ischemia followed by reperfusion; 4. the preconditioning and postconditioning group underwent laparotomy, ischemic preconditioning, 30 minutes of renal ischemia, and ischemic postconditioning followed by reperfusion; and 5. the postconditioning group underwent laparotomy, 30 minutes of renal ischemia, and ischemic postconditioning followed by reperfusion. Serum analyses of creatinine and neutrophil gelatinase-associated lipocalin (NGAL) were performed, and renal histology was examined 24 hours later. Results. Severe tubular injury and increases in creatinine were observed in all groups except the sham group. The control group and all ischemic conditioning groups were no different in the degree of renal injury and values of NGAL and creatinine after the injury. Conclusions. Ischemic preconditioning and ischemic postconditioning, together or separately, are unable to preserve kidney function or exert a protective effect against tubular cell injury after an acute ischemia-reperfusion kidney injury. |
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Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in RatsBackground. Ischemia-reperfusion injury is an unavoidable aspect of transplantation, as well as an important cause of acute kidney injury in clinical practice. Pre- and post-ischemic conditioning are strategies that may provide organs with resistance to major ischemic events. This study evaluates the effects of ischemic preconditioning and ischemic postconditioning, either separately or in combination, after an acute ischemia-reperfusion kidney injury. Methods. Forty Wistar rats received isoflurane anesthesia and were randomized into 5 groups: 1. the sham group underwent laparotomy; 2. the control group underwent laparotomy and 30 minutes of renal ischemia followed by reperfusion; 3. the preconditioning group underwent laparotomy, ischemic preconditioning, and 30 minutes of renal ischemia followed by reperfusion; 4. the preconditioning and postconditioning group underwent laparotomy, ischemic preconditioning, 30 minutes of renal ischemia, and ischemic postconditioning followed by reperfusion; and 5. the postconditioning group underwent laparotomy, 30 minutes of renal ischemia, and ischemic postconditioning followed by reperfusion. Serum analyses of creatinine and neutrophil gelatinase-associated lipocalin (NGAL) were performed, and renal histology was examined 24 hours later. Results. Severe tubular injury and increases in creatinine were observed in all groups except the sham group. The control group and all ischemic conditioning groups were no different in the degree of renal injury and values of NGAL and creatinine after the injury. Conclusions. Ischemic preconditioning and ischemic postconditioning, together or separately, are unable to preserve kidney function or exert a protective effect against tubular cell injury after an acute ischemia-reperfusion kidney injury.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Botucatu Med Sch, Dept Anesthesiol, Sao Paulo, BrazilABC Med Sch, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Pathol, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu Med Sch, Biosci Inst, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Anesthesiol, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu Med Sch, Dept Pathol, Sao Paulo, BrazilUniv Estadual Paulista, Botucatu Med Sch, Biosci Inst, Sao Paulo, BrazilFAPESP: 2012/13603-2FAPESP: 2013/0987-3Elsevier B.V.Universidade Estadual Paulista (Unesp)ABC Med SchArantes, V. M. [UNESP]Bueno, R. T. [UNESP]Modolo, R. P.Domingues, M. A. C. [UNESP]Carvalho, L. R. de [UNESP]Nascimento Junior, P. do [UNESP]Modolo, N. S. P. [UNESP]2019-10-04T12:34:14Z2019-10-04T12:34:14Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3811-3815http://dx.doi.org/10.1016/j.transproceed.2018.07.021Transplantation Proceedings. New York: Elsevier Science Inc, v. 50, n. 10, p. 3811-3815, 2018.0041-1345http://hdl.handle.net/11449/18528010.1016/j.transproceed.2018.07.021WOS:00045497200014587453589896806000000-0002-2323-9159Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransplantation Proceedingsinfo:eu-repo/semantics/openAccess2021-10-23T20:11:43Zoai:repositorio.unesp.br:11449/185280Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T20:11:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
title |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
spellingShingle |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats Arantes, V. M. [UNESP] |
title_short |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
title_full |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
title_fullStr |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
title_full_unstemmed |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
title_sort |
Effects of Ischemic Preconditioning and Postconditioning in a Renal Ischemia-Reperfusion Injury Model: A Comparative Experimental Study in Rats |
author |
Arantes, V. M. [UNESP] |
author_facet |
Arantes, V. M. [UNESP] Bueno, R. T. [UNESP] Modolo, R. P. Domingues, M. A. C. [UNESP] Carvalho, L. R. de [UNESP] Nascimento Junior, P. do [UNESP] Modolo, N. S. P. [UNESP] |
author_role |
author |
author2 |
Bueno, R. T. [UNESP] Modolo, R. P. Domingues, M. A. C. [UNESP] Carvalho, L. R. de [UNESP] Nascimento Junior, P. do [UNESP] Modolo, N. S. P. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) ABC Med Sch |
dc.contributor.author.fl_str_mv |
Arantes, V. M. [UNESP] Bueno, R. T. [UNESP] Modolo, R. P. Domingues, M. A. C. [UNESP] Carvalho, L. R. de [UNESP] Nascimento Junior, P. do [UNESP] Modolo, N. S. P. [UNESP] |
description |
Background. Ischemia-reperfusion injury is an unavoidable aspect of transplantation, as well as an important cause of acute kidney injury in clinical practice. Pre- and post-ischemic conditioning are strategies that may provide organs with resistance to major ischemic events. This study evaluates the effects of ischemic preconditioning and ischemic postconditioning, either separately or in combination, after an acute ischemia-reperfusion kidney injury. Methods. Forty Wistar rats received isoflurane anesthesia and were randomized into 5 groups: 1. the sham group underwent laparotomy; 2. the control group underwent laparotomy and 30 minutes of renal ischemia followed by reperfusion; 3. the preconditioning group underwent laparotomy, ischemic preconditioning, and 30 minutes of renal ischemia followed by reperfusion; 4. the preconditioning and postconditioning group underwent laparotomy, ischemic preconditioning, 30 minutes of renal ischemia, and ischemic postconditioning followed by reperfusion; and 5. the postconditioning group underwent laparotomy, 30 minutes of renal ischemia, and ischemic postconditioning followed by reperfusion. Serum analyses of creatinine and neutrophil gelatinase-associated lipocalin (NGAL) were performed, and renal histology was examined 24 hours later. Results. Severe tubular injury and increases in creatinine were observed in all groups except the sham group. The control group and all ischemic conditioning groups were no different in the degree of renal injury and values of NGAL and creatinine after the injury. Conclusions. Ischemic preconditioning and ischemic postconditioning, together or separately, are unable to preserve kidney function or exert a protective effect against tubular cell injury after an acute ischemia-reperfusion kidney injury. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2019-10-04T12:34:14Z 2019-10-04T12:34:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.transproceed.2018.07.021 Transplantation Proceedings. New York: Elsevier Science Inc, v. 50, n. 10, p. 3811-3815, 2018. 0041-1345 http://hdl.handle.net/11449/185280 10.1016/j.transproceed.2018.07.021 WOS:000454972000145 8745358989680600 0000-0002-2323-9159 |
url |
http://dx.doi.org/10.1016/j.transproceed.2018.07.021 http://hdl.handle.net/11449/185280 |
identifier_str_mv |
Transplantation Proceedings. New York: Elsevier Science Inc, v. 50, n. 10, p. 3811-3815, 2018. 0041-1345 10.1016/j.transproceed.2018.07.021 WOS:000454972000145 8745358989680600 0000-0002-2323-9159 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Transplantation Proceedings |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3811-3815 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964962817310720 |