Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2019.02.004 http://hdl.handle.net/11449/186348 |
Resumo: | The effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Ines ewes (Days 6-7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 mu g of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n = 13) or without (PGF-OT; n = 11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estrous-synchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n = 12) or estradiol cypionate (EC-PGF-OT; n = 12) i.m. along with 37.5 mu g of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P > 0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 +/- 1.1 embryos/ewe collected with mean duration of the flushing procedure being (similar to)36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P > 0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being (similar to)33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P < 0.05). The interval from EB administration to the beginning of transcervical penetration can affect the efficacy of embryo recovery procedures utilizing a combined EB/d-cloprostenol/oxytocin pre-treatment. |
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Repositório Institucional da UNESP |
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Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocinSheepCervical dilationEstradiol estersCloprostenolOxytocinTranscervical embryo recoveryThe effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Ines ewes (Days 6-7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 mu g of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n = 13) or without (PGF-OT; n = 11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estrous-synchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n = 12) or estradiol cypionate (EC-PGF-OT; n = 12) i.m. along with 37.5 mu g of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P > 0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 +/- 1.1 embryos/ewe collected with mean duration of the flushing procedure being (similar to)36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P > 0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being (similar to)33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P < 0.05). The interval from EB administration to the beginning of transcervical penetration can affect the efficacy of embryo recovery procedures utilizing a combined EB/d-cloprostenol/oxytocin pre-treatment.Embrapa Goats and SheepFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Embrapa Caprinos & Ovinos, Estr Sobral Groafras,Km 4,CP D 10, BR-62011000 Sobral, Ceara, BrazilUniv Fed Vicosa, Av PH Rolfs S-N, BR-36570900 Vicosa, MG, BrazilEmbrapa Gado Leite, Rua Eugenio Nascimento 610, BR-36038330 Juiz De Fora, MG, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilUniv Guelph, 50 Stone Rd, Guelph, ON N1G 2W1, CanadaUniv Fed Fluminense, Rua Vital Brazil Filho 64, BR-24230340 Niteroi, RJ, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilEmbrapa Goats and Sheep: 03.12.01.031.00.04FAPEMIG: PPM 00201-17CNPq: 310,166 / 2012-8Elsevier B.V.Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Federal de Viçosa (UFV)Universidade Estadual Paulista (Unesp)Univ GuelphUniversidade Federal Fluminense (UFF)Fonseca, J. F.Zambrini, F. N.Guimaraes, J. D.Silva, M. R.Oliveira, M. E. F. [UNESP]Bartlewski, P. M.Souza-Fabjan, J. M. G.2019-10-04T19:11:40Z2019-10-04T19:11:40Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25-32http://dx.doi.org/10.1016/j.anireprosci.2019.02.004Animal Reproduction Science. Amsterdam: Elsevier, v. 203, p. 25-32, 2019.0378-4320http://hdl.handle.net/11449/18634810.1016/j.anireprosci.2019.02.004WOS:000462810100003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2021-10-22T21:54:12Zoai:repositorio.unesp.br:11449/186348Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T21:54:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
title |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
spellingShingle |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin Fonseca, J. F. Sheep Cervical dilation Estradiol esters Cloprostenol Oxytocin Transcervical embryo recovery |
title_short |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
title_full |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
title_fullStr |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
title_full_unstemmed |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
title_sort |
Cervical penetration rates and efficiency of non-surgical embryo recovery in estrous-synchronized Santa Ines ewes after administration of estradiol ester (benzoate or cypionate) in combination with d-cloprostenol and oxytocin |
author |
Fonseca, J. F. |
author_facet |
Fonseca, J. F. Zambrini, F. N. Guimaraes, J. D. Silva, M. R. Oliveira, M. E. F. [UNESP] Bartlewski, P. M. Souza-Fabjan, J. M. G. |
author_role |
author |
author2 |
Zambrini, F. N. Guimaraes, J. D. Silva, M. R. Oliveira, M. E. F. [UNESP] Bartlewski, P. M. Souza-Fabjan, J. M. G. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Federal de Viçosa (UFV) Universidade Estadual Paulista (Unesp) Univ Guelph Universidade Federal Fluminense (UFF) |
dc.contributor.author.fl_str_mv |
Fonseca, J. F. Zambrini, F. N. Guimaraes, J. D. Silva, M. R. Oliveira, M. E. F. [UNESP] Bartlewski, P. M. Souza-Fabjan, J. M. G. |
dc.subject.por.fl_str_mv |
Sheep Cervical dilation Estradiol esters Cloprostenol Oxytocin Transcervical embryo recovery |
topic |
Sheep Cervical dilation Estradiol esters Cloprostenol Oxytocin Transcervical embryo recovery |
description |
The effects of estradiol esters, D-cloprostenol and oxytocin on induction of cervical dilation prior to non-surgical embryo recovery in Santa Ines ewes (Days 6-7 estrous cycle) were assessed in this study. In Trial 1, transcervical embryo flushing was performed in estrous-induced ewes administered 37.5 mu g of D-cloprostenol i.m. 10 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing with (EB-PGF-OT; n = 13) or without (PGF-OT; n = 11) 1 mg of estradiol benzoate i.m. administered concurrently with D-cloprostenol injection. In Trial 2, the estrous-synchronized animals were treated with 1 mg of estradiol benzoate (EB-PGF-OT; n = 12) or estradiol cypionate (EC-PGF-OT; n = 12) i.m. along with 37.5 mu g of D-cloprostenol i.m. 16 h before and 50 IU of oxytocin i.v. 20 min before uterine flushing. In Trial 1, uterine flushing could be accomplished in 38% of ewes in the EB-PGF-OT and 27% those in the PGF-OT (P > 0.05) group. Flushing fluid recovery averaged 90% and there were 1.0 +/- 1.1 embryos/ewe collected with mean duration of the flushing procedure being (similar to)36 min. In Trial 2, uterine flushing was accomplished in 78% of ewes in the EB-PGF-OT and 44% of those in the EC-PGF-OT group (P > 0.05) with mean flushing fluid recovery rate being 88% and time elapsing to complete flushing being (similar to)33 min. Within the subsets of animals treated with EB, the percentages of successful transcervical penetrations were 38% compared with 78% in Trials 1 and 2, respectively (i.e., with EB administered 10 h compared with 16 h before uterine flushing: P < 0.05). The interval from EB administration to the beginning of transcervical penetration can affect the efficacy of embryo recovery procedures utilizing a combined EB/d-cloprostenol/oxytocin pre-treatment. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T19:11:40Z 2019-10-04T19:11:40Z 2019-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2019.02.004 Animal Reproduction Science. Amsterdam: Elsevier, v. 203, p. 25-32, 2019. 0378-4320 http://hdl.handle.net/11449/186348 10.1016/j.anireprosci.2019.02.004 WOS:000462810100003 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2019.02.004 http://hdl.handle.net/11449/186348 |
identifier_str_mv |
Animal Reproduction Science. Amsterdam: Elsevier, v. 203, p. 25-32, 2019. 0378-4320 10.1016/j.anireprosci.2019.02.004 WOS:000462810100003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25-32 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961856377192448 |