Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612017041 http://hdl.handle.net/11449/179255 |
Resumo: | Hepatozoon species are the most common intracellular hemoparasite found in reptiles. Hepatozoon caimani, whose vectors are Culex mosquitoes, has been detected in a high prevalence among caimans in Brazil by blood smears examinations. The present work aimed to detect and characterize the Hepatozoon spp. found in 33 caimans (24 free-ranging and 9 captive; 28 males and 5 females) (Caiman crocodilus yacare) sampled at Poconé, North Pantanal, state of Mato Grosso, Brazil, using blood smears examinations and molecular techniques. Hepatozoon spp.-gametocytes were found in 70.8% (17/24) and 88.8% (8/9) of blood smears from free-ranging and captive caimans, respectively. Hepatozoon spp. 18S rRNA DNA was found in 79.2% (19/24) and 88.8% (8/9) of free-ranging and captive caimans, respectively. Comparative analysis of parasitized and non-parasitized erythrocytes showed that all analyzed features were significantly different (P<0.05) for both linear and area dimensions. Phylogenetic analysis based on 18S rRNA sequences grouped the Hepatozoon spp. sequences detected in the present study together with H. caimani, recently detected in caimans in southern Pantanal. |
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Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, BrazilHepatozoon caimani em caiman crocodilus yacare (crocodylia, alligatoridae) do norte do pantanal, BrasilBrazilCaimansHepatozoon caimaniMolecular characterisationMorphologyMorphometryHepatozoon species are the most common intracellular hemoparasite found in reptiles. Hepatozoon caimani, whose vectors are Culex mosquitoes, has been detected in a high prevalence among caimans in Brazil by blood smears examinations. The present work aimed to detect and characterize the Hepatozoon spp. found in 33 caimans (24 free-ranging and 9 captive; 28 males and 5 females) (Caiman crocodilus yacare) sampled at Poconé, North Pantanal, state of Mato Grosso, Brazil, using blood smears examinations and molecular techniques. Hepatozoon spp.-gametocytes were found in 70.8% (17/24) and 88.8% (8/9) of blood smears from free-ranging and captive caimans, respectively. Hepatozoon spp. 18S rRNA DNA was found in 79.2% (19/24) and 88.8% (8/9) of free-ranging and captive caimans, respectively. Comparative analysis of parasitized and non-parasitized erythrocytes showed that all analyzed features were significantly different (P<0.05) for both linear and area dimensions. Phylogenetic analysis based on 18S rRNA sequences grouped the Hepatozoon spp. sequences detected in the present study together with H. caimani, recently detected in caimans in southern Pantanal.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPFaculdade de Ciências Agrárias e Veterinárias-FCAV Universidade Estadual Paulista-UNESPFAPESP: 2015/14896-1Universidade Estadual Paulista (Unesp)Bouer, Andréa [UNESP]André, Marcos Rogério [UNESP]Gonçalves, Luiz Ricardo [UNESP]Luzzi, Mayara de Cássia [UNESP]De Oliveira, Juliana Paula [UNESP]Rodrigues, Adriana Carlos [UNESP]Varani, Alessandro De Melo [UNESP]De Miranda, Vitor Fernandes Oliveira [UNESP]Perles, Lívia [UNESP]Werther, Karin [UNESP]Machado, Rosangela Zacarias [UNESP]2018-12-11T17:34:24Z2018-12-11T17:34:24Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article352-358application/pdfhttp://dx.doi.org/10.1590/S1984-29612017041Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 352-358, 2017.0103-846Xhttp://hdl.handle.net/11449/17925510.1590/S1984-29612017041S1984-296120170003003522-s2.0-85030662456S1984-29612017000300352.pdf884680349956281991398998955805130000-0002-8822-488XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-11-21T06:14:23Zoai:repositorio.unesp.br:11449/179255Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-21T06:14:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil Hepatozoon caimani em caiman crocodilus yacare (crocodylia, alligatoridae) do norte do pantanal, Brasil |
title |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil |
spellingShingle |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil Bouer, Andréa [UNESP] Brazil Caimans Hepatozoon caimani Molecular characterisation Morphology Morphometry |
title_short |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil |
title_full |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil |
title_fullStr |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil |
title_full_unstemmed |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil |
title_sort |
Hepatozoon caimani in caiman crocodilus yacare (crocodylia, alligatoridae) from north pantanal, Brazil |
author |
Bouer, Andréa [UNESP] |
author_facet |
Bouer, Andréa [UNESP] André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Luzzi, Mayara de Cássia [UNESP] De Oliveira, Juliana Paula [UNESP] Rodrigues, Adriana Carlos [UNESP] Varani, Alessandro De Melo [UNESP] De Miranda, Vitor Fernandes Oliveira [UNESP] Perles, Lívia [UNESP] Werther, Karin [UNESP] Machado, Rosangela Zacarias [UNESP] |
author_role |
author |
author2 |
André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Luzzi, Mayara de Cássia [UNESP] De Oliveira, Juliana Paula [UNESP] Rodrigues, Adriana Carlos [UNESP] Varani, Alessandro De Melo [UNESP] De Miranda, Vitor Fernandes Oliveira [UNESP] Perles, Lívia [UNESP] Werther, Karin [UNESP] Machado, Rosangela Zacarias [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bouer, Andréa [UNESP] André, Marcos Rogério [UNESP] Gonçalves, Luiz Ricardo [UNESP] Luzzi, Mayara de Cássia [UNESP] De Oliveira, Juliana Paula [UNESP] Rodrigues, Adriana Carlos [UNESP] Varani, Alessandro De Melo [UNESP] De Miranda, Vitor Fernandes Oliveira [UNESP] Perles, Lívia [UNESP] Werther, Karin [UNESP] Machado, Rosangela Zacarias [UNESP] |
dc.subject.por.fl_str_mv |
Brazil Caimans Hepatozoon caimani Molecular characterisation Morphology Morphometry |
topic |
Brazil Caimans Hepatozoon caimani Molecular characterisation Morphology Morphometry |
description |
Hepatozoon species are the most common intracellular hemoparasite found in reptiles. Hepatozoon caimani, whose vectors are Culex mosquitoes, has been detected in a high prevalence among caimans in Brazil by blood smears examinations. The present work aimed to detect and characterize the Hepatozoon spp. found in 33 caimans (24 free-ranging and 9 captive; 28 males and 5 females) (Caiman crocodilus yacare) sampled at Poconé, North Pantanal, state of Mato Grosso, Brazil, using blood smears examinations and molecular techniques. Hepatozoon spp.-gametocytes were found in 70.8% (17/24) and 88.8% (8/9) of blood smears from free-ranging and captive caimans, respectively. Hepatozoon spp. 18S rRNA DNA was found in 79.2% (19/24) and 88.8% (8/9) of free-ranging and captive caimans, respectively. Comparative analysis of parasitized and non-parasitized erythrocytes showed that all analyzed features were significantly different (P<0.05) for both linear and area dimensions. Phylogenetic analysis based on 18S rRNA sequences grouped the Hepatozoon spp. sequences detected in the present study together with H. caimani, recently detected in caimans in southern Pantanal. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T17:34:24Z 2018-12-11T17:34:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612017041 Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 352-358, 2017. 0103-846X http://hdl.handle.net/11449/179255 10.1590/S1984-29612017041 S1984-29612017000300352 2-s2.0-85030662456 S1984-29612017000300352.pdf 8846803499562819 9139899895580513 0000-0002-8822-488X |
url |
http://dx.doi.org/10.1590/S1984-29612017041 http://hdl.handle.net/11449/179255 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 26, n. 3, p. 352-358, 2017. 0103-846X 10.1590/S1984-29612017041 S1984-29612017000300352 2-s2.0-85030662456 S1984-29612017000300352.pdf 8846803499562819 9139899895580513 0000-0002-8822-488X |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
352-358 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965021218799616 |