Análise da regeneração óssea alveolar de ratas ovariectomizadas tratadas com diferentes concentrações de estradiol

• Autor(a) principal: Caliente, Eliana Aparecida [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149486; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000856880.pdf
• topic: Estradiol; Ovariectomia; Regeneração óssea

In this study it was verified the estradiol action at concentrations of 100, 200 and 300 μg/mm³/month. It was also evaluated the breast and uterine tumor incidence and the possible correlation with the concentration named above, with the aim to define the level to be used in the hormone replacement therapy of ovariectomized rats. Therefore Wistar rats (6 months) with regular estrous cycle were selected and ovariectomized OVX or sham-ovariectomized, kept at room temperature (22 ± 2°C), with controlled light cycle (12/12h) and free access to water and food. After 10 days, the animals were distributed in groups for the hormone replacement therapy and received subcutaneous implant containing corn oil with 100, 200 or 300μg/mm³ of 17β-estradiol, during 90 days, changing every 30 days. After 62 days it was performed a maxillary right central incisor extraction for a further analysis of alveolar bone repair. Twenty-eight days after the extraction blood was collected to obtain serum and plasma and then performed euthanasia on the animals to collect the maxillaries. The maxillary bone was prepared to make the slides and further histological analysis (HE and immunohistochemistry). Serum and plasma were stored to determine the bone markers. It was not detected tumors in the estrogenized animals. It was verified that in the shams and OVX animals which received 200 e 300 μg/mm³ of E2 the markers for osteocalcin and OPG were more intense. However in the group animals of OVX rats which received pellets containing corn oil those markers were subtle being more intense the markers for TRAP. In the analysis of the stained slides with the hematoxylin and eosin, it was possible to verify regular bone formation in the groups treated with 200 e 300 μg/mm³ of estradiol. The results obtained in this study suggest the use of 200 μg/mm³ estrogen concentration for bone metabolism studies