MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS
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Publication Date: | 2014 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.3732/apps.1300073 http://hdl.handle.net/11449/117524 |
Summary: | Premise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species.Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species.Conclusions: Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats. |
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MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPSBromeliaceaecross-amplificationgene flowgenetic diversityOrthophytum ophiuroidespopulation geneticsPremise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species.Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species.Conclusions: Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Inst Bot, BR-04301902 Sao Paulo, BrazilUniv Fed Pernambuco, Dept Bot, BR-50670901 Recife, PE, BrazilUniv Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, BrazilUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, BrazilUniv Estadual Paulista, Dept Ecol, BR-13506900 Rio Claro, SP, BrazilFAPESP: 11/06438-2FAPESP: 09/52725-3FAPESP: 09/17411-8Botanical Soc Amer IncInst BotUniversidade Federal de Pernambuco (UFPE)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Aoki-Goncalves, FelipeLouzada, Rafael B.De Souza, Livia MouraPalma-Silva, Clarisse [UNESP]2015-03-18T15:56:21Z2015-03-18T15:56:21Z2014-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4application/pdfhttp://dx.doi.org/10.3732/apps.1300073Applications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 4 p., 2014.2168-0450http://hdl.handle.net/11449/11752410.3732/apps.1300073WOS:000342559000002WOS000342559000002.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplications In Plant Sciences1.1870,746info:eu-repo/semantics/openAccess2023-10-27T06:10:08Zoai:repositorio.unesp.br:11449/117524Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:10:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
title |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
spellingShingle |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS Aoki-Goncalves, Felipe Bromeliaceae cross-amplification gene flow genetic diversity Orthophytum ophiuroides population genetics |
title_short |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
title_full |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
title_fullStr |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
title_full_unstemmed |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
title_sort |
MICROSATELLITE LOCI FOR ORTHOPHYTUM OPHIUROIDES (BROMELIOIDEAE, BROMELIACEAE) SPECIES ADAPTED TO NEOTROPICAL ROCK OUTCROPS |
author |
Aoki-Goncalves, Felipe |
author_facet |
Aoki-Goncalves, Felipe Louzada, Rafael B. De Souza, Livia Moura Palma-Silva, Clarisse [UNESP] |
author_role |
author |
author2 |
Louzada, Rafael B. De Souza, Livia Moura Palma-Silva, Clarisse [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Inst Bot Universidade Federal de Pernambuco (UFPE) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Aoki-Goncalves, Felipe Louzada, Rafael B. De Souza, Livia Moura Palma-Silva, Clarisse [UNESP] |
dc.subject.por.fl_str_mv |
Bromeliaceae cross-amplification gene flow genetic diversity Orthophytum ophiuroides population genetics |
topic |
Bromeliaceae cross-amplification gene flow genetic diversity Orthophytum ophiuroides population genetics |
description |
Premise of the study: Microsatellite primers were developed for Orthophytum ophiuroides, a rupicolous bromeliad species endemic to neotropical rocky fields. These microsatellite loci will be used to investigate population differentiation and species cohesion in such fragmented environments. The loci were tested for cross-amplification in related bromeliad species.Methods and Results: Eleven polymorphic microsatellite markers were isolated and characterized from an enriched library of O. ophiuroides. The loci were tested on 42 individuals from two populations of this species. The number of alleles per locus ranged from three to nine and the expected and observed heterozygosities ranged from 0.167 to 0.870 and from 0.369 to 0.958, respectively. Seven loci successfully amplified in other related bromeliad species.Conclusions: Our results suggest that the microsatellite loci developed here will be useful to assess genetic diversity and gene flow in O. ophiuroides for the investigation of population differentiation and species cohesion in neotropical mountainous habitats. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-03-01 2015-03-18T15:56:21Z 2015-03-18T15:56:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3732/apps.1300073 Applications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 4 p., 2014. 2168-0450 http://hdl.handle.net/11449/117524 10.3732/apps.1300073 WOS:000342559000002 WOS000342559000002.pdf |
url |
http://dx.doi.org/10.3732/apps.1300073 http://hdl.handle.net/11449/117524 |
identifier_str_mv |
Applications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 4 p., 2014. 2168-0450 10.3732/apps.1300073 WOS:000342559000002 WOS000342559000002.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applications In Plant Sciences 1.187 0,746 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4 application/pdf |
dc.publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
publisher.none.fl_str_mv |
Botanical Soc Amer Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789529598328832 |