Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1984-29612016009 http://hdl.handle.net/11449/172740 |
Resumo: | The aim of this work was a correlation study and histopathological description of alterations associated with the presence of Leishmania infantum amastigote in the intestinal wall of dogs infected with canine visceral leishmaniasis (CVL). Three groups were used: G1 (n = 8), comprising naturally infected dogs with CVL with amastigotes of L. infantum in the small and large intestines; G2 (n = 9), infected dogs with CVL, without intestinal amastigotes; and G3 (n = 3), uninfected dogs. Histochemistry and immunohistochemistry methods were used for histopathology and amastigotes identification. 47.1% (8/17) of dogs from G1 group had amastigotes in the mucosa, submucosa and muscle layers of the small and large intestines and it was observed a prominent inflammatory reaction characterized by chronic infiltration of mononuclear cells: macrophages, lymphocytes and plasma cells. Comparison between the groups showed only a significant difference in relation to mucosal microscopic structural alterations in dogs from G1 in relation to G2 and G3. Parasite burden showed significant correlations with the microscopic alterations and clinical status of dogs in G1. By the conclusion, the inflammatory reactions caused by the parasites in the intestines might have contributed towards alterations in digestive processes, worsening the dogs’ clinical status of CVL. |
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Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantumCorrelation study and histopathological description of intestinal alterations in dogs infected with leishmania infantumAmastigotesDogHistologyImmunohistochemistryIntestineLeishmaniasisThe aim of this work was a correlation study and histopathological description of alterations associated with the presence of Leishmania infantum amastigote in the intestinal wall of dogs infected with canine visceral leishmaniasis (CVL). Three groups were used: G1 (n = 8), comprising naturally infected dogs with CVL with amastigotes of L. infantum in the small and large intestines; G2 (n = 9), infected dogs with CVL, without intestinal amastigotes; and G3 (n = 3), uninfected dogs. Histochemistry and immunohistochemistry methods were used for histopathology and amastigotes identification. 47.1% (8/17) of dogs from G1 group had amastigotes in the mucosa, submucosa and muscle layers of the small and large intestines and it was observed a prominent inflammatory reaction characterized by chronic infiltration of mononuclear cells: macrophages, lymphocytes and plasma cells. Comparison between the groups showed only a significant difference in relation to mucosal microscopic structural alterations in dogs from G1 in relation to G2 and G3. Parasite burden showed significant correlations with the microscopic alterations and clinical status of dogs in G1. By the conclusion, the inflammatory reactions caused by the parasites in the intestines might have contributed towards alterations in digestive processes, worsening the dogs’ clinical status of CVL.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia e Zootecnia Universidade Estadual Paulista – UNESP, Campus de Ilha SolteiraFundação Educacional de Andradina – FEADepartamento de Matemática Universidade Estadual Paulista – UNESP, Campus de Ilha SolteiraCentro de Controle de ZoonosesDepartamento de Biologia e Zootecnia Universidade Estadual Paulista – UNESP, Campus de Ilha SolteiraDepartamento de Matemática Universidade Estadual Paulista – UNESP, Campus de Ilha SolteiraFAPESP: 2013/13875-5Universidade Estadual Paulista (Unesp)Fundação Educacional de Andradina – FEACentro de Controle de ZoonosesSilva, Diogo Tiago [UNESP]Neves, Maria Francisca [UNESP]de Queiroz, Nina Mari Gual Pimenta [UNESP]Spada, Julio Cesar Pereira [UNESP]Alves, Maria Luana [UNESP]Flóro e Silva, Marina [UNESP]Coelho, Willian Marinho DouradoPanosso, Alan Rodrigo [UNESP]Noronha Junior, Antonio Carlos FacontiStarke-Buzetti, Wilma Aparecida [UNESP]2018-12-11T17:01:59Z2018-12-11T17:01:59Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24-36application/pdfhttp://dx.doi.org/10.1590/S1984-29612016009Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 1, p. 24-36, 2016.0103-846Xhttp://hdl.handle.net/11449/17274010.1590/S1984-29612016009S1984-296120160001000242-s2.0-84961942953S1984-29612016000100024.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-10-18T06:04:20Zoai:repositorio.unesp.br:11449/172740Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-18T06:04:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum Correlation study and histopathological description of intestinal alterations in dogs infected with leishmania infantum |
title |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum |
spellingShingle |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum Silva, Diogo Tiago [UNESP] Amastigotes Dog Histology Immunohistochemistry Intestine Leishmaniasis |
title_short |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum |
title_full |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum |
title_fullStr |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum |
title_full_unstemmed |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum |
title_sort |
Estudo de correlação e descrição histopatológica das lesões intestinais de cães infectados com Leishmania infantum |
author |
Silva, Diogo Tiago [UNESP] |
author_facet |
Silva, Diogo Tiago [UNESP] Neves, Maria Francisca [UNESP] de Queiroz, Nina Mari Gual Pimenta [UNESP] Spada, Julio Cesar Pereira [UNESP] Alves, Maria Luana [UNESP] Flóro e Silva, Marina [UNESP] Coelho, Willian Marinho Dourado Panosso, Alan Rodrigo [UNESP] Noronha Junior, Antonio Carlos Faconti Starke-Buzetti, Wilma Aparecida [UNESP] |
author_role |
author |
author2 |
Neves, Maria Francisca [UNESP] de Queiroz, Nina Mari Gual Pimenta [UNESP] Spada, Julio Cesar Pereira [UNESP] Alves, Maria Luana [UNESP] Flóro e Silva, Marina [UNESP] Coelho, Willian Marinho Dourado Panosso, Alan Rodrigo [UNESP] Noronha Junior, Antonio Carlos Faconti Starke-Buzetti, Wilma Aparecida [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Fundação Educacional de Andradina – FEA Centro de Controle de Zoonoses |
dc.contributor.author.fl_str_mv |
Silva, Diogo Tiago [UNESP] Neves, Maria Francisca [UNESP] de Queiroz, Nina Mari Gual Pimenta [UNESP] Spada, Julio Cesar Pereira [UNESP] Alves, Maria Luana [UNESP] Flóro e Silva, Marina [UNESP] Coelho, Willian Marinho Dourado Panosso, Alan Rodrigo [UNESP] Noronha Junior, Antonio Carlos Faconti Starke-Buzetti, Wilma Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Amastigotes Dog Histology Immunohistochemistry Intestine Leishmaniasis |
topic |
Amastigotes Dog Histology Immunohistochemistry Intestine Leishmaniasis |
description |
The aim of this work was a correlation study and histopathological description of alterations associated with the presence of Leishmania infantum amastigote in the intestinal wall of dogs infected with canine visceral leishmaniasis (CVL). Three groups were used: G1 (n = 8), comprising naturally infected dogs with CVL with amastigotes of L. infantum in the small and large intestines; G2 (n = 9), infected dogs with CVL, without intestinal amastigotes; and G3 (n = 3), uninfected dogs. Histochemistry and immunohistochemistry methods were used for histopathology and amastigotes identification. 47.1% (8/17) of dogs from G1 group had amastigotes in the mucosa, submucosa and muscle layers of the small and large intestines and it was observed a prominent inflammatory reaction characterized by chronic infiltration of mononuclear cells: macrophages, lymphocytes and plasma cells. Comparison between the groups showed only a significant difference in relation to mucosal microscopic structural alterations in dogs from G1 in relation to G2 and G3. Parasite burden showed significant correlations with the microscopic alterations and clinical status of dogs in G1. By the conclusion, the inflammatory reactions caused by the parasites in the intestines might have contributed towards alterations in digestive processes, worsening the dogs’ clinical status of CVL. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:01:59Z 2018-12-11T17:01:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612016009 Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 1, p. 24-36, 2016. 0103-846X http://hdl.handle.net/11449/172740 10.1590/S1984-29612016009 S1984-29612016000100024 2-s2.0-84961942953 S1984-29612016000100024.pdf |
url |
http://dx.doi.org/10.1590/S1984-29612016009 http://hdl.handle.net/11449/172740 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 25, n. 1, p. 24-36, 2016. 0103-846X 10.1590/S1984-29612016009 S1984-29612016000100024 2-s2.0-84961942953 S1984-29612016000100024.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
24-36 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964603322466304 |