Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri

Detalhes bibliográficos
Autor(a) principal: Saldanha, Luiz Leonardo
Data de Publicação: 2022
Outros Autores: Allard, Pierre-Marie, Dilarri, Guilherme, Codesido, Santiago, González-Ruiz, Víctor, Queiroz, Emerson Ferreira, Ferreira, Henrique, Wolfender, Jean-Luc
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1021/acs.jafc.2c05156
http://hdl.handle.net/11449/249001
Resumo: Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (X. citri), is a plant disease affecting Citrus crops worldwide. However, little is known about defense compounds in Citrus. Here, we conducted a mass spectrometry-based metabolomic approach to obtain an overview of the chemical responses of Citrus leaves to X. citri infection. To facilitate result interpretation, the multivariate analyses were combined with molecular networking to identify biomarkers. Metabolite variations among untreated and X. citri-inoculated Citrus samples under greenhouse conditions highlighted induced defense biomarkers. Notably, the plant tryptophan metabolism pathway was activated, leading to the accumulation of N-methylated tryptamine derivatives. This finding was subsequently confirmed in symptomatic leaves in the field. Several tryptamine derivatives showed inhibitory effects in vitro against X. citri. This approach has enabled the identification of new chemically related biomarker groups and their dynamics in the response of Citrus leaves to Xanthomonas infection.
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spelling Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citribiotic stresscitrus cankerpathogen-induced metabolitessweet orangetryptaminesCitrus canker, caused by the bacterium Xanthomonas citri subsp. citri (X. citri), is a plant disease affecting Citrus crops worldwide. However, little is known about defense compounds in Citrus. Here, we conducted a mass spectrometry-based metabolomic approach to obtain an overview of the chemical responses of Citrus leaves to X. citri infection. To facilitate result interpretation, the multivariate analyses were combined with molecular networking to identify biomarkers. Metabolite variations among untreated and X. citri-inoculated Citrus samples under greenhouse conditions highlighted induced defense biomarkers. Notably, the plant tryptophan metabolism pathway was activated, leading to the accumulation of N-methylated tryptamine derivatives. This finding was subsequently confirmed in symptomatic leaves in the field. Several tryptamine derivatives showed inhibitory effects in vitro against X. citri. This approach has enabled the identification of new chemically related biomarker groups and their dynamics in the response of Citrus leaves to Xanthomonas infection.Biochemistry Building Department of General and Applied Biology Institute of Biosciences State University of São Paulo, Rio ClaroInstitute of Pharmaceutical Sciences of Western Switzerland University of GenevaSchool of Pharmaceutical Sciences University of GenevaDepartement of Biology University of FribourgUniversidade de São Paulo (USP)University of GenevaUniversity of FribourgSaldanha, Luiz LeonardoAllard, Pierre-MarieDilarri, GuilhermeCodesido, SantiagoGonzález-Ruiz, VíctorQueiroz, Emerson FerreiraFerreira, HenriqueWolfender, Jean-Luc2023-07-29T13:59:40Z2023-07-29T13:59:40Z2022-11-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14693-14705http://dx.doi.org/10.1021/acs.jafc.2c05156Journal of Agricultural and Food Chemistry, v. 70, n. 46, p. 14693-14705, 2022.1520-51180021-8561http://hdl.handle.net/11449/24900110.1021/acs.jafc.2c051562-s2.0-85141941548Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Agricultural and Food Chemistryinfo:eu-repo/semantics/openAccess2023-07-29T13:59:40Zoai:repositorio.unesp.br:11449/249001Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:59:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
title Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
spellingShingle Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
Saldanha, Luiz Leonardo
biotic stress
citrus canker
pathogen-induced metabolites
sweet orange
tryptamines
title_short Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
title_full Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
title_fullStr Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
title_full_unstemmed Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
title_sort Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri
author Saldanha, Luiz Leonardo
author_facet Saldanha, Luiz Leonardo
Allard, Pierre-Marie
Dilarri, Guilherme
Codesido, Santiago
González-Ruiz, Víctor
Queiroz, Emerson Ferreira
Ferreira, Henrique
Wolfender, Jean-Luc
author_role author
author2 Allard, Pierre-Marie
Dilarri, Guilherme
Codesido, Santiago
González-Ruiz, Víctor
Queiroz, Emerson Ferreira
Ferreira, Henrique
Wolfender, Jean-Luc
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
University of Geneva
University of Fribourg
dc.contributor.author.fl_str_mv Saldanha, Luiz Leonardo
Allard, Pierre-Marie
Dilarri, Guilherme
Codesido, Santiago
González-Ruiz, Víctor
Queiroz, Emerson Ferreira
Ferreira, Henrique
Wolfender, Jean-Luc
dc.subject.por.fl_str_mv biotic stress
citrus canker
pathogen-induced metabolites
sweet orange
tryptamines
topic biotic stress
citrus canker
pathogen-induced metabolites
sweet orange
tryptamines
description Citrus canker, caused by the bacterium Xanthomonas citri subsp. citri (X. citri), is a plant disease affecting Citrus crops worldwide. However, little is known about defense compounds in Citrus. Here, we conducted a mass spectrometry-based metabolomic approach to obtain an overview of the chemical responses of Citrus leaves to X. citri infection. To facilitate result interpretation, the multivariate analyses were combined with molecular networking to identify biomarkers. Metabolite variations among untreated and X. citri-inoculated Citrus samples under greenhouse conditions highlighted induced defense biomarkers. Notably, the plant tryptophan metabolism pathway was activated, leading to the accumulation of N-methylated tryptamine derivatives. This finding was subsequently confirmed in symptomatic leaves in the field. Several tryptamine derivatives showed inhibitory effects in vitro against X. citri. This approach has enabled the identification of new chemically related biomarker groups and their dynamics in the response of Citrus leaves to Xanthomonas infection.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-23
2023-07-29T13:59:40Z
2023-07-29T13:59:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1021/acs.jafc.2c05156
Journal of Agricultural and Food Chemistry, v. 70, n. 46, p. 14693-14705, 2022.
1520-5118
0021-8561
http://hdl.handle.net/11449/249001
10.1021/acs.jafc.2c05156
2-s2.0-85141941548
url http://dx.doi.org/10.1021/acs.jafc.2c05156
http://hdl.handle.net/11449/249001
identifier_str_mv Journal of Agricultural and Food Chemistry, v. 70, n. 46, p. 14693-14705, 2022.
1520-5118
0021-8561
10.1021/acs.jafc.2c05156
2-s2.0-85141941548
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Agricultural and Food Chemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14693-14705
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1797790019445850112

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