Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: A proteomic analyses

• Autor(a) principal: Magalhães, Lizandra G.
• Data de Publicação: 2016
• Outros Autores: Lima, Thais C., de Paula, Renato G., Morais, Enyara R., Aguiar, Daniela P., Gardinassi, Luiz G., Garcia, Gustavo R., Laurentiz, Rosangela S., Rodrigues, Vanderlei, Bastos, Jairo K., Filho, Ademar A. S., Yatsuda, Ana P., Cunha, Wilson R., Silva, Márcio L. A.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.bjp.2016.02.001; http://hdl.handle.net/11449/220643
• topic: (−)-6;6’-dinitrohinokinin; Lignan; Mass spectrometry; Proteome; Schistosoma mansoni; Two-dimensional gel electrophoresis

Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematodeflukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monother-apy with praziquantel reinforces the need for search and development of new therapeutic drugs.Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosomamansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansonisoluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry.Enrichment for biological processes and predictive analyses of protein-protein interactions suggest thatdibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes,especially carbohydrate metabolism. In summary, this study provides an interesting approach to under-stand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound fromlignan and for the development of new therapy strategies.