Cell death is involved in sexual dimorphism during preimplantation development
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mod.2015.12.001 http://hdl.handle.net/11449/158739 |
Resumo: | In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120 h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144 h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process. (C) 2016 Elsevier Ireland Ltd. All rights reserved. |
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Cell death is involved in sexual dimorphism during preimplantation developmentSexual dimorphismPreimplantation developmentFemale embryoApoptosisCaspase 3In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120 h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144 h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process. (C) 2016 Elsevier Ireland Ltd. All rights reserved.Foundation for Research Support of the State of Rio de JaneiroEmbrapa Dairy Cattle, Anim Reprod Lab, Valenca, BrazilEmbrapa Amazonia Oriental, Belem, Para, BrazilSao Paulo State Univ, Jaboticabal, BrazilUniv Sao Paulo, Pirassununga, BrazilCtr Univ Rio Preto UNIRP, Sao Jose Do Rio Preto, BrazilPESAGRO Rio, Anim Reprod Lab, Valenca, BrazilSao Paulo State Univ, Jaboticabal, BrazilFoundation for Research Support of the State of Rio de Janeiro: FAPERJ 111.361Elsevier B.V.Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Ctr Univ Rio Preto UNIRPPESAGRO RioOliveira, C. S.Saraiva, N. Z.Lima, M. R. de [UNESP]Oliveira, L. Z.Serapiao, R. V.Garcia, J. M. [UNESP]Borges, C. A. V.Camargo, L. S. A.2018-11-26T15:28:50Z2018-11-26T15:28:50Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article42-50application/pdfhttp://dx.doi.org/10.1016/j.mod.2015.12.001Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 139, p. 42-50, 2016.0925-4773http://hdl.handle.net/11449/15873910.1016/j.mod.2015.12.001WOS:000371800400005WOS000371800400005.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMechanisms Of Development1,049info:eu-repo/semantics/openAccess2023-12-24T06:20:42Zoai:repositorio.unesp.br:11449/158739Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:22.889774Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cell death is involved in sexual dimorphism during preimplantation development |
title |
Cell death is involved in sexual dimorphism during preimplantation development |
spellingShingle |
Cell death is involved in sexual dimorphism during preimplantation development Oliveira, C. S. Sexual dimorphism Preimplantation development Female embryo Apoptosis Caspase 3 |
title_short |
Cell death is involved in sexual dimorphism during preimplantation development |
title_full |
Cell death is involved in sexual dimorphism during preimplantation development |
title_fullStr |
Cell death is involved in sexual dimorphism during preimplantation development |
title_full_unstemmed |
Cell death is involved in sexual dimorphism during preimplantation development |
title_sort |
Cell death is involved in sexual dimorphism during preimplantation development |
author |
Oliveira, C. S. |
author_facet |
Oliveira, C. S. Saraiva, N. Z. Lima, M. R. de [UNESP] Oliveira, L. Z. Serapiao, R. V. Garcia, J. M. [UNESP] Borges, C. A. V. Camargo, L. S. A. |
author_role |
author |
author2 |
Saraiva, N. Z. Lima, M. R. de [UNESP] Oliveira, L. Z. Serapiao, R. V. Garcia, J. M. [UNESP] Borges, C. A. V. Camargo, L. S. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Ctr Univ Rio Preto UNIRP PESAGRO Rio |
dc.contributor.author.fl_str_mv |
Oliveira, C. S. Saraiva, N. Z. Lima, M. R. de [UNESP] Oliveira, L. Z. Serapiao, R. V. Garcia, J. M. [UNESP] Borges, C. A. V. Camargo, L. S. A. |
dc.subject.por.fl_str_mv |
Sexual dimorphism Preimplantation development Female embryo Apoptosis Caspase 3 |
topic |
Sexual dimorphism Preimplantation development Female embryo Apoptosis Caspase 3 |
description |
In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120 h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144 h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process. (C) 2016 Elsevier Ireland Ltd. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-02-01 2018-11-26T15:28:50Z 2018-11-26T15:28:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mod.2015.12.001 Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 139, p. 42-50, 2016. 0925-4773 http://hdl.handle.net/11449/158739 10.1016/j.mod.2015.12.001 WOS:000371800400005 WOS000371800400005.pdf |
url |
http://dx.doi.org/10.1016/j.mod.2015.12.001 http://hdl.handle.net/11449/158739 |
identifier_str_mv |
Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 139, p. 42-50, 2016. 0925-4773 10.1016/j.mod.2015.12.001 WOS:000371800400005 WOS000371800400005.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mechanisms Of Development 1,049 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
42-50 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129296250699776 |