Cell death is involved in sexual dimorphism during preimplantation development

Detalhes bibliográficos
Autor(a) principal: Oliveira, C. S.
Data de Publicação: 2016
Outros Autores: Saraiva, N. Z., Lima, M. R. de [UNESP], Oliveira, L. Z., Serapiao, R. V., Garcia, J. M. [UNESP], Borges, C. A. V., Camargo, L. S. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mod.2015.12.001
http://hdl.handle.net/11449/158739
Resumo: In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120 h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144 h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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spelling Cell death is involved in sexual dimorphism during preimplantation developmentSexual dimorphismPreimplantation developmentFemale embryoApoptosisCaspase 3In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120 h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144 h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process. (C) 2016 Elsevier Ireland Ltd. All rights reserved.Foundation for Research Support of the State of Rio de JaneiroEmbrapa Dairy Cattle, Anim Reprod Lab, Valenca, BrazilEmbrapa Amazonia Oriental, Belem, Para, BrazilSao Paulo State Univ, Jaboticabal, BrazilUniv Sao Paulo, Pirassununga, BrazilCtr Univ Rio Preto UNIRP, Sao Jose Do Rio Preto, BrazilPESAGRO Rio, Anim Reprod Lab, Valenca, BrazilSao Paulo State Univ, Jaboticabal, BrazilFoundation for Research Support of the State of Rio de Janeiro: FAPERJ 111.361Elsevier B.V.Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Ctr Univ Rio Preto UNIRPPESAGRO RioOliveira, C. S.Saraiva, N. Z.Lima, M. R. de [UNESP]Oliveira, L. Z.Serapiao, R. V.Garcia, J. M. [UNESP]Borges, C. A. V.Camargo, L. S. A.2018-11-26T15:28:50Z2018-11-26T15:28:50Z2016-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article42-50application/pdfhttp://dx.doi.org/10.1016/j.mod.2015.12.001Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 139, p. 42-50, 2016.0925-4773http://hdl.handle.net/11449/15873910.1016/j.mod.2015.12.001WOS:000371800400005WOS000371800400005.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMechanisms Of Development1,049info:eu-repo/semantics/openAccess2023-12-24T06:20:42Zoai:repositorio.unesp.br:11449/158739Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:11:22.889774Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cell death is involved in sexual dimorphism during preimplantation development
title Cell death is involved in sexual dimorphism during preimplantation development
spellingShingle Cell death is involved in sexual dimorphism during preimplantation development
Oliveira, C. S.
Sexual dimorphism
Preimplantation development
Female embryo
Apoptosis
Caspase 3
title_short Cell death is involved in sexual dimorphism during preimplantation development
title_full Cell death is involved in sexual dimorphism during preimplantation development
title_fullStr Cell death is involved in sexual dimorphism during preimplantation development
title_full_unstemmed Cell death is involved in sexual dimorphism during preimplantation development
title_sort Cell death is involved in sexual dimorphism during preimplantation development
author Oliveira, C. S.
author_facet Oliveira, C. S.
Saraiva, N. Z.
Lima, M. R. de [UNESP]
Oliveira, L. Z.
Serapiao, R. V.
Garcia, J. M. [UNESP]
Borges, C. A. V.
Camargo, L. S. A.
author_role author
author2 Saraiva, N. Z.
Lima, M. R. de [UNESP]
Oliveira, L. Z.
Serapiao, R. V.
Garcia, J. M. [UNESP]
Borges, C. A. V.
Camargo, L. S. A.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Ctr Univ Rio Preto UNIRP
PESAGRO Rio
dc.contributor.author.fl_str_mv Oliveira, C. S.
Saraiva, N. Z.
Lima, M. R. de [UNESP]
Oliveira, L. Z.
Serapiao, R. V.
Garcia, J. M. [UNESP]
Borges, C. A. V.
Camargo, L. S. A.
dc.subject.por.fl_str_mv Sexual dimorphism
Preimplantation development
Female embryo
Apoptosis
Caspase 3
topic Sexual dimorphism
Preimplantation development
Female embryo
Apoptosis
Caspase 3
description In bovine preimplantation development, female embryos progress at lower rates and originate smaller blastocysts than male counterparts. Although sex-specific gene expression patterns are reported, when and how sex dimorphism is established is not clear. Differences among female and male early development can be useful for human assisted reproductive medicine, when X-linked disorders risk is detected, and for genetic breeding programs, especially in dairy cattle, which requires female animals for milk production. The aim of this study was to characterize the development of female and male embryos, attempting to identify sex effects during preimplantation development and the role of cell death in this process. Using sex-sorted semen from three different bulls for fertilization, we compared kinetics of bovine sex-specific embryos in six time points, and cell death was assessed in viable embryos. For kinetics analysis, we detected an increased population of female embryos arrested at 48 and 120 h.p.i., suggesting this time points as delicate stages of development for female embryos that should be considered for testing improvement strategies for assisted reproductive technologies. Assessing viable embryos quality, we found 144 h.p.i. is the first time point when viable embryos are phenotypically distinct: cell number is decreased, and apoptosis and cell fragmentation are increased in female embryos at this stage. These new results lead us to propose that sex dimorphism in viable embryos is established during morula-blastocyst transition, and cell death is involved in this process. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-01
2018-11-26T15:28:50Z
2018-11-26T15:28:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mod.2015.12.001
Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 139, p. 42-50, 2016.
0925-4773
http://hdl.handle.net/11449/158739
10.1016/j.mod.2015.12.001
WOS:000371800400005
WOS000371800400005.pdf
url http://dx.doi.org/10.1016/j.mod.2015.12.001
http://hdl.handle.net/11449/158739
identifier_str_mv Mechanisms Of Development. Amsterdam: Elsevier Science Bv, v. 139, p. 42-50, 2016.
0925-4773
10.1016/j.mod.2015.12.001
WOS:000371800400005
WOS000371800400005.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mechanisms Of Development
1,049
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 42-50
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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